Subcellular Localization and Functional Analysis of the Arabidopsis GTPase RabE

Speth, Elena Bray; Imboden, Lori; Hauck, Paula M.; He, Sheng Yang

doi:10.1104/pp.108.132092

Cited by 111 publications

(124 citation statements)

References 77 publications

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“…Therefore, a unique identity of the SM could allow the membrane to intercept specific secretory traffic to the plasma membrane and specific endocytic/biosynthetic traffic toward the vacuole (83). The presence of Rab8 and Rab2 small GTPases, that are thought to be involved in trafficking of vesicles from the Golgi and the ER, respectively, to the plasma membrane (85)(86)(87), further supports the idea of the SM as a chimeric membrane.…”

Section: Soybean Symbiosome Proteomementioning

confidence: 64%

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

Clarke

Loughlin

Gavrin

et al. 2015

Molecular & Cellular Proteomics

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Legumes form a symbiosis with rhizobia in which the plant provides an energy source to the rhizobia bacteria that it uses to fix atmospheric nitrogen. This nitrogen is provided to the legume plant, allowing it to grow without the addition of nitrogen fertilizer. As part of the symbiosis, the bacteria in the infected cells of a new root organ, the nodule, are surrounded by a plant-derived membrane, the symbiosome membrane, which becomes the interface between the symbionts. Fractions containing the symbiosome membrane (SM) and material from the lumen of the symbiosome (peribacteroid space or PBS) were isolated from soybean root nodules and analyzed using nongel proteomic techniques. Bicarbonate stripping and chloroform-methanol extraction of isolated SM were used to reduce complexity of the samples and enrich for hydrophobic integral membrane proteins. One hundred and ninety-seven proteins were identified as components of the SM, with an additional fifteen proteins identified from peripheral membrane and PBS protein fractions. Proteins involved in a range of cellular processes such as metabolism, protein folding and degradation, membrane trafficking, and solute transport were identified. These included a number of proteins previously localized to the SM, such as aquaglyceroporin nodulin 26, sulfate transporters, remorin, and Rab7 homologs. Among the proteome were a number of putative transporters for compounds such as sulfate, calcium, hydrogen ions, peptide/ dicarboxylate, and nitrate, as well as transporters for which the substrate is not easy to predict. Analysis of the promoter activity for six genes encoding putative SM proteins showed nodule specific expression, with five showing expression only in infected cells. Localization of two proteins was confirmed using GFP-fusion experiments. The data have been deposited to the ProteomeXchange with identifier PXD001132. This proteome will provide a rich resource for the study of the legumerhizobium symbiosis. Molecular & Cellular Proteomics

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Section: Soybean Symbiosome Proteomementioning

confidence: 64%

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

Clarke

Loughlin

Gavrin

et al. 2015

Molecular & Cellular Proteomics

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“…A cursory database search identified over 200 articles in which the gl1 background has been used to study molecular, genetic, and biochemical aspects of diverse aspects of plant physiology (Liu and Zhu, 1997;Aarts et al, 1998;Bender and Fink, 1998;Cecchini et al, 1998;Xie et al, 1998;Yi and Jack, 1998;Bieza and Lois, 2001;Ellis and Turner, 2001;Laby et al, 2001;Devoto et al, 2002;He et al, 2002;Collins et al, 2003;Quiel and Bender, 2003;Barkan et al, 2006;Zhu et al, 2007;Speth et al, 2009;Jung and Niyogi, 2010;Rowe et al, 2010). Notably, many of the mutants that were isolated or crossed into the gl1 background have been studied for the defense physiologies.…”

Section: Discussionmentioning

confidence: 99%

The glabra1 Mutation Affects Cuticle Formation and Plant Responses to Microbes

Xia

Navarre

et al. 2010

Plant Physiology

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Systemic acquired resistance (SAR) is a form of defense that provides resistance against a broad spectrum of pathogens in plants. Previous work indicates a role for plastidial glycerolipid biosynthesis in SAR. Specifically, mutations in FATTY ACID DESATURASE7 (FAD7), which lead to reduced trienoic fatty acid levels and compromised plastidial lipid biosynthesis, have been associated with defective SAR. We show that the defective SAR in Arabidopsis (Arabidopsis thaliana) fad7-1 plants is not associated with a mutation in FAD7 but rather with a second-site mutation in GLABRA1 (GL1), a gene well known for its role in trichome formation. The compromised SAR in gl1 plants is associated with impairment in their cuticles. Furthermore, mutations in two other components of trichome development, GL3 and TRANSPARENT TESTA GLABRA1, also impaired cuticle development and SAR. This suggests an overlap in the biochemical pathways leading to cuticle and trichome development. Interestingly, exogenous application of gibberellic acid (GA) not only enhanced SAR in wild-type plants but also restored SAR in gl1 plants. In contrast to GA, the defense phytohoromes salicylic acid and jasmonic acid were unable to restore SAR in gl1 plants. GA application increased levels of cuticular components but not trichome formation on gl1 plants, thus implicating cuticle, but not trichomes, as an important component of SAR. Our findings question the prudence of using mutant backgrounds for genetic screens and underscore a need to reevaluate phenotypes previously studied in the gl1 background.

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“…Thus it is reasonable to speculate that PIP5K2 is the main source of PtdIns(4,5)P 2 production in roots. In addition, PIP5K2 shows a preferential subcellular localization at the plasma membrane, forms aggregates in the BFA compartment after BFA treatment, and interacts with RabE GTPases which are involved in anterograde membrane trafficking [69,70], indicating a possible role of PIP5K2 in vesicle trafficking. Our observations confirm that the internalization of the membrane marker FM4-64 is suppressed in pip5k2; this functionality can be recovered by both exogenous PtdIns(4,5)P 2 treatment and complementation by PIP5K2 expression ( Figure 5A).…”

Section: Pip5k2/ptdins(45)p 2 Regulates Vesicle Trafficking and Is Cmentioning

confidence: 99%

Arabidopsis phosphatidylinositol monophosphate 5-kinase 2 is involved in root gravitropism through regulation of polar auxin transport by affecting the cycling of PIN proteins

et al. 2011

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Phosphatidylinositol monophosphate 5-kinase (PIP5K) catalyzes the synthesis of PI-4,5-bisphosphate (PtdIns(4,5) P 2 ) by phosphorylation of PI-4-phosphate at the 5 position of the inositol ring, and is involved in regulating multiple developmental processes and stress responses. We here report on the functional characterization of Arabidopsis PIP5K2, which is expressed during lateral root initiation and elongation, and whose expression is enhanced by exogenous auxin. The knockout mutant pip5k2 shows reduced lateral root formation, which could be recovered with exogenous auxin, and interestingly, delayed root gravity response that could not be recovered with exogenous auxin. Crossing with the DR5-GUS marker line and measurement of free IAA content confirmed the reduced auxin accumulation in pip5k2. In addition, analysis using the membrane-selective dye FM4-64 revealed the decelerated vesicle trafficking caused by PtdIns(4,5)P 2 reduction, which hence results in suppressed cycling of PIN proteins (PIN2 and 3), and delayed redistribution of PIN2 and auxin under gravistimulation in pip5k2 roots. On the contrary, PtdIns(4,5) P 2 significantly enhanced the vesicle trafficking and cycling of PIN proteins. These results demonstrate that PIP5K2 is involved in regulating lateral root formation and root gravity response, and reveal a critical role of PIP5K2/PtdIns(4,5)P 2 in root development through regulation of PIN proteins, providing direct evidence of crosstalk between the phosphatidylinositol signaling pathway and auxin response, and new insights into the control of polar auxin transport.

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Subcellular Localization and Functional Analysis of the Arabidopsis GTPase RabE

Cited by 111 publications

References 77 publications

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

The glabra1 Mutation Affects Cuticle Formation and Plant Responses to Microbes

Arabidopsis phosphatidylinositol monophosphate 5-kinase 2 is involved in root gravitropism through regulation of polar auxin transport by affecting the cycling of PIN proteins

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