Subcellular localization and trafficking of kainate receptors

Jaskolski, Frédéric; Coussen, Françoise; Mulle, Christophe

doi:10.1016/j.tips.2004.11.008

Cited by 94 publications

(81 citation statements)

References 60 publications

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“…Although kainate receptors along axons are possible, these are usually located close to active zones [78]. Kainic acid-induced neuronal death in the rat hippocampus is accompanied by an increase in the proliferative rates of astrocytes and microglia [79], but rather not by a complete destruction of by-passing axons.…”

Section: Discussionmentioning

confidence: 99%

Reduction in 50-kHz call-numbers and suppression of tickling-associated positive affective behaviour after lesioning of the lateral hypothalamic parvafox nucleus in rats

Roccaro-Waldmeyer

Babalian

Müller

et al. 2016

Behavioural Brain Research

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h i g h l i g h t s• Wistar rats were tickled before and after excitotoxic lesioning of the lateral hypothalamic parvafox nucleus.• Ultrasonic vocalisations (USVs) that were emitted during the tickling of rats and their tendency to approach and follow the experimenter's hand were analysed.• Lesioning was considered successful if the number of parvalbuminimmunoreactive (PV-ir) cells in the area of the parvafox nucleus was reduced beyond a threshold level.• Rats with bilaterally successful lesions manifested the most profound surgery-associated reduction in the number of 50-kHz USVs and in the tendency to approach and follow the experimenter's hand.• Positive correlations were found between each of the four investigated parameters. g r a p h i c a l a b s t r a c tThe parvafox nucleus is located ventrolaterally in the lateral hypothalamic area (LHA). Its core and shell are composed of neurons expressing the calcium-binding protein parvalbumin (PV) and the transcription factor Foxb1, respectively. Given the known functions of the LHA and that the parvafox nucleus receives afferents from the lateral orbitofrontal cortex and projects to the periaqueductal gray matter, a functional role of this entity in the expression of positive emotions has been postulated.The purpose of the present study was to ascertain whether the deletion of neurons in the parvafox nucleus influenced the tickling-induced 50-kHz calls, which are thought to reflect positive affective states, in rats. To this end, tickling of the animals (heterospecific play) was combined with intracerebral injections of the excitotoxin kainic acid into the parvafox nucleus.The most pronounced surgery-associated reduction in 50-kHz call-numbers was observed in the group of rats in which, on the basis of PV-immunoreactive-cell counts in the parvafox nucleus, bilateral lesions had been successfully produced. Two other parameters that were implemented to quantify positive affective behaviour, namely, an approach towards and a following of the hand of the tickling * Corresponding author at: Institute of Anatomy, University of Fribourg, Rte A. Gockel 1, CH-1700 Fribourg, Switzerland.E-mail address: marco.celio@unifr.ch (M.R. Celio). 1Published in which should be cited to refer to this work.http://doc.rero.ch experimenter, were likewise most markedly suppressed in the group of rats with bilaterally successful lesions. Furthermore, positive correlations were found between each of the investigated parameters. Our data afford evidence that the parvafox nucleus plays a role in the production of 50-kHz calls in rats, and, more generally, in the expression of positive emotions.

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Section: Discussionmentioning

confidence: 99%

Reduction in 50-kHz call-numbers and suppression of tickling-associated positive affective behaviour after lesioning of the lateral hypothalamic parvafox nucleus in rats

Roccaro-Waldmeyer

Babalian

Müller

et al. 2016

Behavioural Brain Research

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“…The KA2 ER retention/retrieval signal consists primarily of an arginine-rich domain similar to that characterized on several other ionotropic glutamate receptor subunits, including NR1 (5) and GluR5-2c (6), as well as other signaling proteins such as ATP-sensitive potassium channels (K ATP ) (7) and GABA B receptors (8). Although a number of trafficking motifs in KAR subunits have been identified (9), little is known about how these trafficking signals control localization of receptors and which chaperone proteins are responsible for the modulation of receptor trafficking.…”

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confidence: 99%

Intracellular Trafficking of KA2 Kainate Receptors Mediated by Interactions with Coatomer Protein Complex I (COPI) and 14-3-3 Chaperone Systems

Vivithanaporn

Yan

Swanson

2006

Journal of Biological Chemistry

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Assembly and trafficking of neurotransmitter receptors are processes contingent upon interactions between intracellular chaperone systems and discrete determinants in the receptor proteins. Kainate receptor subunits, which form ionotropic glutamate receptors with diverse roles in the central nervous system, contain a variety of trafficking determinants that promote either membrane expression or intracellular sequestration. In this report, we identify the coatomer protein complex I (COPI) vesicle coat as a critical mechanism for retention of the kainate receptor subunit KA2 in the endoplasmic reticulum. COPI subunits immunoprecipitated with KA2 subunits from both cerebellum and COS-7 cells, and ␤-COP protein interacted directly with immobilized KA2 peptides containing the arginine-rich retention/retrieval determinant. Association between COPI proteins and KA2 subunits was significantly reduced upon alanine substitution of this signal in the cytoplasmic tail of KA2. Temperature-sensitive degradation of COPI complex proteins was correlated with an increase in plasma membrane localization of the homologous KA2 receptor. Assembly of heteromeric GluR6a/KA2 receptors markedly reduced association of KA2 and COPI. Finally, the reduction in COPI binding was correlated with an increased association with 14-3-3 proteins, which mediate forward trafficking of other integral signaling proteins. These interactions therefore represent a critical early checkpoint for biosynthesis of functional KARs. Kainate receptors (KARs)2 play a variety of roles in the mammalian central nervous system that include contributions to postsynaptic neurotransmission at a subset of excitatory synapses and presynaptic modulation of both excitatory and inhibitory transmission (1, 2). These diverse physiological roles of KARs require selective assembly, subcellular trafficking, and targeting of these proteins to their functional sites. Elucidating the cellular mechanisms that control these processes is important for understanding the full spectrum of KAR-mediated signaling in the brain.Oligomerization and intracellular trafficking of KARs are controlled in part through interactions with chaperone proteins that bind to discrete cytoplasmic domains on the receptor proteins themselves. For example, GluR6a KAR subunits contain a forward trafficking determinant in their cytoplasmic tail and therefore are highly expressed as homomeric receptors on the plasma membrane of heterologous cell lines (3), whereas the KA2 subunit has an endoplasmic reticulum (ER) retention/retrieval signal and consequently does not reach the plasma membrane in the absence of other KAR subunits (4). The KA2 ER retention/retrieval signal consists primarily of an arginine-rich domain similar to that characterized on several other ionotropic glutamate receptor subunits, including NR1 (5) and GluR5-2c (6), as well as other signaling proteins such as ATP-sensitive potassium channels (K ATP ) (7) and GABA B receptors (8). Although a number of trafficking motifs in KAR subunits have been id...

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“…[14][15][16] Reduced Q/R editing of GluK2 results in increased calcium permeability and altered I/V relations. 17 Thus, KARs comprise a diverse combination of subunits and subunit isoforms that are subject to different sets of protein interactions leading to differential trafficking and surface expression, thereby influencing the fate and function of the receptors in which they assemble [3][4][5]7,8 ( Figure 2). …”

Section: Advanced Reviewmentioning

confidence: 99%

“…To date, most localization studies have used electrophysiological approaches and it seems that, in general, presynaptic KARs modulate neurotransmitter release while postsynaptic KARs mediate excitatory neurotransmission. 4,5,22 Hippocampal CA3 pyramidal and CA1 interneurons are among the most extensively studied neurons. Both cell types contain pre-and postsynaptic KARs and illustrate the diverse polarized trafficking of KARs even within the same neuron.…”

Section: Roles and Distribution Of Kars Localizationmentioning

confidence: 99%

Kainate receptor trafficking

González‐González

Konopacki

Rocca

et al. 2011

WIREs Membr Transp Signal

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Kainate receptors (KARs) are ligand-gated ion channels that can regulate neuronal network activity and are involved in processes ranging from neuronal development and differentiation to neurodegeneration and neuronal cell death. They are tetrameric assemblies which can comprise different subunit combinations and are differentially targeted to specific cell surface compartments including preand postsynaptic membranes where they perform distinct functional roles. The processes regulating the constitutive and activity-dependent trafficking of KARs are subtle and complex. Intricate combinations of protein-protein interactions and post-translational modifications orchestrate their surface membrane delivery, residence time, internalization, and recycling or degradation. Furthermore, in addition to regulating surface expression, interacting proteins connect receptors to anchoring and signaling pathways. Although our knowledge of the processes that regulate KAR trafficking is far from complete, there has been significant progress toward defining the roles of many of these protein partners and post-translational modifications. 

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Subcellular localization and trafficking of kainate receptors

Cited by 94 publications

References 60 publications

Reduction in 50-kHz call-numbers and suppression of tickling-associated positive affective behaviour after lesioning of the lateral hypothalamic parvafox nucleus in rats

Reduction in 50-kHz call-numbers and suppression of tickling-associated positive affective behaviour after lesioning of the lateral hypothalamic parvafox nucleus in rats

Intracellular Trafficking of KA2 Kainate Receptors Mediated by Interactions with Coatomer Protein Complex I (COPI) and 14-3-3 Chaperone Systems

Kainate receptor trafficking

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