Subcellular mechanism of microbial inactivation during water disinfection by cold atmospheric-pressure plasma

Xu, Huanjun; Zhu, Yupan; Du, Mengru; Wang, Yuqi; Ju, Siyao; Ma, Ruonan; Zhang, Jiao

doi:10.1016/j.watres.2020.116513

Cited by 108 publications

(49 citation statements)

References 103 publications

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“…Trion selectively interacts with and is oxidized by superoxide, allowing its effective removal from the solution (26,27). Our finding is supported by previous research showing that superoxide anions and downstream reactive species such as singlet molecular oxygen are significant contributors to the antimicrobial activity of PAW produced by dialectic barrier discharge against yeast cells (28) and Salmonella Typhimurium (29).…”

Section: Discussionsupporting

confidence: 90%

The Antimicrobial Efficacy Of Plasma Activated Water Is Modulated By Reactor Design And Water Composition

Alam

Carter

et al. 2021

Preprint

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Plasma activated water (PAW) contains a cocktail of reactive oxidative species and free radicals and has demonstrated efficacy as a sanitizer for fresh produce, however there is a need for further optimization. The antimicrobial efficacy of PAW produced by a bubble spark discharge (BSD) reactor and a dielectric barrier discharge-diffuser (DBDD) reactor operating at atmospheric conditions with air, discharge frequencies of 500, 1000 and 1500 Hz, and MilliQ and tap water, was investigated with model organisms Listeria innocua and Escherichia coli. Optimal conditions were subsequently employed for pathogenic bacteria Listeria monocytogenes, E. coli and Salmonella enterica. PAW generated with the DBDD reactor decontaminated more than 6-log CFU of bacteria within 1 minute of treatment. The BSD-PAW, while attaining high CFU reduction was less effective, particularly for L. innocua. Analysis of physicochemical properties revealed BSD-PAW had a greater variety of reactive species than DBDD-PAW. Scavenger assays were employed to specifically sequester reactive species, including the short-lived superoxide (?O2-) radical that could not be directly measured in the PAW. This demonstrated a critical role of superoxide for the inactivation of both E. coli and L. innocua by DBDD-PAW, while in BSD-PAW it had a role in L. innocua inactivation only. Overall, this study demonstrates the potential of DBDD-PAW in fresh produce, where there is a need for sterilization while minimizing chemical inputs and residues and maintaining food quality. Highly effective PAW was generated using air as a processing gas and tap water, making this a feasible and cost-effective option.

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Section: Discussionsupporting

confidence: 90%

The Antimicrobial Efficacy Of Plasma Activated Water Is Modulated By Reactor Design And Water Composition

Alam

Carter

et al. 2021

Preprint

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“…Although it has not been specifically shown that intracellular membranes of yeast are damaged by plasma, it is highly probable. An increase in intracellular Ca 2+ observed in plasma-treated cells [47] can likely be attributed to the disruption of the barrier function of cellular membranes. Staining of yeast cells with TMRM or JC-1 further indicated that, in plasma-treated yeast cells, mitochondria may not be able to maintain transmembrane potential (∆ψ) [36,47], resulting in a dramatic decrease in cellular ATP level [47].…”

Section: Effect Of Plasma On Membranes and Energy Metabolismmentioning

confidence: 99%

“…An increase in intracellular Ca 2+ observed in plasma-treated cells [47] can likely be attributed to the disruption of the barrier function of cellular membranes. Staining of yeast cells with TMRM or JC-1 further indicated that, in plasma-treated yeast cells, mitochondria may not be able to maintain transmembrane potential (∆ψ) [36,47], resulting in a dramatic decrease in cellular ATP level [47]. This may be, indeed, caused by the damage to the inner mitochondrial membrane due to lipid peroxidation.…”

Section: Effect Of Plasma On Membranes and Energy Metabolismmentioning

confidence: 99%

“…Yeast S. cereviseae (similar to E. coli) do not produce polyunsaturated fatty acids with more than one double bond [45,46], but the generation of MDA was described in the plasma-treated yeast cells [36,47], indicating that yeast lipids get peroxidated under these conditions. In the same study, it was also shown that the same treatment leads to the leakage of proteins as well as potassium ions (K + ) from the treated cells to the surrounding medium, to the loss of the membrane potential at cytoplasmic membrane and to an increase in the intracellular pH.…”

Section: Effect Of Plasma On Membranes and Energy Metabolismmentioning

confidence: 99%

“…This may be, indeed, caused by the damage to the inner mitochondrial membrane due to lipid peroxidation. On the other hand, numerous causes other than membrane damage could explain the loss of membrane potential, including the changes in metabolism of the cell due to oxidative damage of many cellular enzymes or, as it has also been suggested [47], by opening of the mitochondrial permeability transition pore (PTP). As the latter is a highconductance nonspecific channel in mitochondrial membranes that opens in response to Ca 2+ [48], its opening and participation on the dissipation of mitochondrial ∆ψ is consistent with the increase in intracellular Ca 2+ .…”

Section: Effect Of Plasma On Membranes and Energy Metabolismmentioning

confidence: 99%

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Effects of Non-Thermal Plasma on Yeast Saccharomyces cerevisiae

Polčic

Machala

2021

IJMS

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Cold plasmas generated by various electrical discharges can affect cell physiology or induce cell damage that may often result in the loss of viability. Many cold plasma-based technologies have emerged in recent years that are aimed at manipulating the cells within various environments or tissues. These include inactivation of microorganisms for the purpose of sterilization, food processing, induction of seeds germination, but also the treatment of cells in the therapy. Mechanisms that underlie the plasma-cell interactions are, however, still poorly understood. Dissection of cellular pathways or structures affected by plasma using simple eukaryotic models is therefore desirable. Yeast Saccharomyces cerevisiae is a traditional model organism with unprecedented impact on our knowledge of processes in eukaryotic cells. As such, it had been also employed in studies of plasma-cell interactions. This review focuses on the effects of cold plasma on yeast cells.

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Photothermal and Photocatalytic Glycol Chitosan and Polydopamine‐Grafted Oxygen Vacancy Bismuth Oxyiodide (BiO_1‐xI) Nanoparticles for the Diagnosis and Targeted Therapy of Diabetic Wounds

Kong,

Ma,

Chu

et al. 2023

Advanced Materials

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Treatment of diabetic wounds is a significant clinical challenge due to the massive infections caused by bacteria. In this study, multifunctional glycol chitosan and polydopamine‐coated BiO1‐xI (GPBO) nanoparticles (NPs) with near‐infrared (NIR) photothermal and photocatalytic abilities are prepared. When infection occurs, the local microenvironment becomes acidic, and the pH‐switchable GPBO can target the bacteria of the wound site. The NIR‐assisted GPBO treatment exhibits anti‐bacterial effects with fast response, high efficiency, and long duration to Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. GPBO achieves excellent photothermal imaging and CT imaging of the mouse subcutaneous abscess model. With the assistance of NIR irradiation, the GPBO promotes the healing of the diabetic wound model with the effects of anti‐bacteria, anti‐inflammation, the M2 polarization promotion of macrophages, and angiogenesis. This is the first‐time report of nano‐sized BiO1‐xI. The synthesis and selected application for the imaging and targeted therapy of diabetic wounds are presented. This study offers an example of the NP‐assisted precise diagnosis and therapy of bacterial infection diseases.

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Subcellular mechanism of microbial inactivation during water disinfection by cold atmospheric-pressure plasma

Cited by 108 publications

References 103 publications

The Antimicrobial Efficacy Of Plasma Activated Water Is Modulated By Reactor Design And Water Composition

The Antimicrobial Efficacy Of Plasma Activated Water Is Modulated By Reactor Design And Water Composition

Effects of Non-Thermal Plasma on Yeast Saccharomyces cerevisiae

Photothermal and Photocatalytic Glycol Chitosan and Polydopamine‐Grafted Oxygen Vacancy Bismuth Oxyiodide (BiO_1‐xI) Nanoparticles for the Diagnosis and Targeted Therapy of Diabetic Wounds

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Subcellular mechanism of microbial inactivation during water disinfection by cold atmospheric-pressure plasma

Cited by 108 publications

References 103 publications

The Antimicrobial Efficacy Of Plasma Activated Water Is Modulated By Reactor Design And Water Composition

The Antimicrobial Efficacy Of Plasma Activated Water Is Modulated By Reactor Design And Water Composition

Effects of Non-Thermal Plasma on Yeast Saccharomyces cerevisiae

Photothermal and Photocatalytic Glycol Chitosan and Polydopamine‐Grafted Oxygen Vacancy Bismuth Oxyiodide (BiO1‐xI) Nanoparticles for the Diagnosis and Targeted Therapy of Diabetic Wounds

Contact Info

Product

Resources

About

Photothermal and Photocatalytic Glycol Chitosan and Polydopamine‐Grafted Oxygen Vacancy Bismuth Oxyiodide (BiO_1‐xI) Nanoparticles for the Diagnosis and Targeted Therapy of Diabetic Wounds