Subcellular mislocalization of the transcription factor NF-E2 in erythroid cells discriminates prefibrotic primary myelofibrosis from essential thrombocythemia

Abstract

Key Points The transcription factor NF-E2 is mislocalized in patients with primary myelofibrosis. Immunohistochemical staining for NF-E2 distinguishes essential thrombocythemia from primary myelofibrosis.

Nuclear factor-erythroid 2, nerve growth factor receptor, and CD34–microvessel density are differentially expressed in primary myelofibrosis, polycythemia vera, and essential thrombocythemia

“…In the ET and PMF groups, NF-E2 overexpression was not correlated with the status of JAK2 mutation or allele burden, which also appeared unrelated for PV cases. Only rare myeloid precursors and a variable proportion of megakaryocytes in the sections were highlighted as seen in one of previously published study [9]. The megakaryocytes had the similar staining patterns among all the groups.…”

“…Aumann et al [9] investigated immunohistochemical expression levels of NF-E2 in MPNs. According to their findings, the highest nuclear staining was found in PMF (33.7% ± 10.7%) followed by PV (23.9% ± 10%) and ET (16.3% ± 4.1%), respectively.…”

“…This discrepancy may be caused by the usage of different fixative solutions. Our samples were all fixed in Bouin solution, whereas in a previous study we referenced, either formalin or calcium-glutaraldehydeformaldehyde was used [9]. Also, decalcifying agents may have some effect on the tissues which may result in such discordance on immunohistochemistry.…”

“…More recently, a study from the same group described potential diagnostic usefulness of immunostaining for NF-E2 in separating subtypes of MPN using BM biopsies [9].…”

A novel role for nuclear factor-erythroid 2 in erythroid maturation by modulation of mitochondrial autophagy

“…36 Only 10% of all erythroid cells, mainly the early erythroblasts, showed nuclear NF-E2 staining. So far, no function has been attributed to the cytoplasmically located NF-E2 protein.…”