Subchronic effects of environment-like cadmium levels on the bivalve<i>Anodonta anatina</i>(Linnaeus 1758): III. Effects on carbonic anhydrase activity in relation to calcium metabolism

Ngo, Huong Thi Thuy; Gerstmann, Silke; Frank, Hartmut

doi:10.1080/02772240802503619

Cited by 16 publications

(8 citation statements)

References 25 publications

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“…This suggests that absorption of toxic Cd 2+ from the surrounding substrate via the alimentary tract may constitute a particular physiological challenge, exacerbated by the evolutionary transition of gastropods to terrestrial life [ 36 ]. Moreover, the sensitivity to Cd 2+ of important Cu-dominated metabolic pathways [ 54 - 56 ] and Zn-dependent enzymes [ 57 ] could have been the basis of the generation of a specific MT isoform devoted to Cd 2+ detoxification in these animals. Our data also demonstrate the ready formation of homometallic complexes of HpCdMT with Zn 2+ , which may be a consequence of the comparable coordination preferences of these two d 10 metal ions.…”

Section: Discussionmentioning

confidence: 99%

Shaping mechanisms of metal specificity in a family of metazoan metallothioneins: evolutionary differentiation of mollusc metallothioneins

et al. 2011

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BackgroundThe degree of metal binding specificity in metalloproteins such as metallothioneins (MTs) can be crucial for their functional accuracy. Unlike most other animal species, pulmonate molluscs possess homometallic MT isoforms loaded with Cu+ or Cd2+. They have, so far, been obtained as native metal-MT complexes from snail tissues, where they are involved in the metabolism of the metal ion species bound to the respective isoform. However, it has not as yet been discerned if their specific metal occupation is the result of a rigid control of metal availability, or isoform expression programming in the hosting tissues or of structural differences of the respective peptides determining the coordinative options for the different metal ions. In this study, the Roman snail (Helix pomatia) Cu-loaded and Cd-loaded isoforms (HpCuMT and HpCdMT) were used as model molecules in order to elucidate the biochemical and evolutionary mechanisms permitting pulmonate MTs to achieve specificity for their cognate metal ion.ResultsHpCuMT and HpCdMT were recombinantly synthesized in the presence of Cd2+, Zn2+ or Cu2+ and corresponding metal complexes analysed by electrospray mass spectrometry and circular dichroism (CD) and ultra violet-visible (UV-Vis) spectrophotometry. Both MT isoforms were only able to form unique, homometallic and stable complexes (Cd6-HpCdMT and Cu12-HpCuMT) with their cognate metal ions. Yeast complementation assays demonstrated that the two isoforms assumed metal-specific functions, in agreement with their binding preferences, in heterologous eukaryotic environments. In the snail organism, the functional metal specificity of HpCdMT and HpCuMT was contributed by metal-specific transcription programming and cell-specific expression. Sequence elucidation and phylogenetic analysis of MT isoforms from a number of snail species revealed that they possess an unspecific and two metal-specific MT isoforms, whose metal specificity was achieved exclusively by evolutionary modulation of non-cysteine amino acid positions.ConclusionThe Roman snail HpCdMT and HpCuMT isoforms can thus be regarded as prototypes of isoform families that evolved genuine metal-specificity within pulmonate molluscs. Diversification into these isoforms may have been initiated by gene duplication, followed by speciation and selection towards opposite needs for protecting copper-dominated metabolic pathways from nonessential cadmium. The mechanisms enabling these proteins to be metal-specific could also be relevant for other metalloproteins.

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Section: Discussionmentioning

confidence: 99%

Shaping mechanisms of metal specificity in a family of metazoan metallothioneins: evolutionary differentiation of mollusc metallothioneins

et al. 2011

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“…e mechanisms by which heavy metals a ect di erent organs, tissues, and systems in di erent organisms are very complex, and so far some of them are not fully explored. Exposure of the bivalve Anodonta anatina to Cd has been found to a ect carbonic anhydrase (CA) in its tissues, an enzyme playing a role in osmoregulation and Ca metabolism [60]. Cd has been considered as one of the factors likely responsible for the decline in populations of freshwater mussels due to its high toxicity, bioaccumulation potential, and transfer through food chains [61].…”

Section: Heavy Metals As Hazardous Materialsmentioning

confidence: 99%

Environmental Chemistry and Ecotoxicology of Hazardous Heavy Metals: Environmental Persistence, Toxicity, and Bioaccumulation

Ali

Khan

Ilahi

2019

Journal of Chemistry

1,836

832

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Heavy metals are well-known environmental pollutants due to their toxicity, persistence in the environment, and bioaccumulative nature. Their natural sources include weathering of metal-bearing rocks and volcanic eruptions, while anthropogenic sources include mining and various industrial and agricultural activities. Mining and industrial processing for extraction of mineral resources and their subsequent applications for industrial, agricultural, and economic development has led to an increase in the mobilization of these elements in the environment and disturbance of their biogeochemical cycles. Contamination of aquatic and terrestrial ecosystems with toxic heavy metals is an environmental problem of public health concern. Being persistent pollutants, heavy metals accumulate in the environment and consequently contaminate the food chains. Accumulation of potentially toxic heavy metals in biota causes a potential health threat to their consumers including humans. This article comprehensively reviews the different aspects of heavy metals as hazardous materials with special focus on their environmental persistence, toxicity for living organisms, and bioaccumulative potential. The bioaccumulation of these elements and its implications for human health are discussed with a special coverage on fish, rice, and tobacco. The article will serve as a valuable educational resource for both undergraduate and graduate students and for researchers in environmental sciences. Environmentally relevant most hazardous heavy metals and metalloids include Cr, Ni, Cu, Zn, Cd, Pb, Hg, and As. The trophic transfer of these elements in aquatic and terrestrial food chains/webs has important implications for wildlife and human health. It is very important to assess and monitor the concentrations of potentially toxic heavy metals and metalloids in different environmental segments and in the resident biota. A comprehensive study of the environmental chemistry and ecotoxicology of hazardous heavy metals and metalloids shows that steps should be taken to minimize the impact of these elements on human health and the environment.

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“…Divalent metal uptake was inhibited by elevated Ca 2+ in freshwater bivalves, and Ca 2+ uptake was inhibited by divalent metals in L. stagnalis ( Supplemental Data, Table S5 ). Elevated [Ca 2+ ] in the hemolymph and extrapallial fluids occurs, because Cd 2+ inhibits PMCA [ 216 ] and CA [ 217 ], which assist in movement of dissolved Ca 2+ into the shell and into concretions in the soft tissues ( Supplemental Data, Table S5 ).…”

Section: Transporters and Physiology Of Individual Ionsmentioning

confidence: 99%

Toxicological perspective on the osmoregulation and ionoregulation physiology of major ions by freshwater animals: Teleost fish, crustacea, aquatic insects, and Mollusca

Griffith

2016

Enviro Toxic and Chemistry

146

136

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Anthropogenic sources increase freshwater salinity and produce differences in constituent ions compared with natural waters. Moreover, ions differ in physiological roles and concentrations in intracellular and extracellular fluids. Four freshwater taxa groups are compared, to investigate similarities and differences in ion transport processes and what ion transport mechanisms suggest about the toxicity of these or other ions in freshwater. Although differences exist, many ion transporters are functionally similar and may belong to evolutionarily conserved protein families. For example, the Na+/H+-exchanger in teleost fish differs from the H+/2Na+ (or Ca2+)-exchanger in crustaceans. In osmoregulation, Na+ and Cl− predominate. Stenohaline freshwater animals hyperregulate until they are no longer able to maintain hypertonic extracellular Na+ and Cl− concentrations with increasing salinity and become isotonic. Toxic effects of K+ are related to ionoregulation and volume regulation. The ionic balance between intracellular and extracellular fluids is maintained by Na+/K+-adenosine triphosphatase (ATPase), but details are lacking on apical K+ transporters. Elevated H+ affects the maintenance of internal Na+ by Na+/H+ exchange; elevated HCO3− inhibits Cl− uptake. The uptake of Mg2+ occurs by the gills or intestine, but details are lacking on Mg2+ transporters. In unionid gills, SO42− is actively transported, but most epithelia are generally impermeant to SO42−. Transporters of Ca2+ maintain homeostasis of dissolved Ca2+. More integration of physiology with toxicology is needed to fully understand freshwater ion effects.

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Subchronic effects of environment-like cadmium levels on the bivalveAnodonta anatina(Linnaeus 1758): III. Effects on carbonic anhydrase activity in relation to calcium metabolism

Cited by 16 publications

References 25 publications

Shaping mechanisms of metal specificity in a family of metazoan metallothioneins: evolutionary differentiation of mollusc metallothioneins

Shaping mechanisms of metal specificity in a family of metazoan metallothioneins: evolutionary differentiation of mollusc metallothioneins

Environmental Chemistry and Ecotoxicology of Hazardous Heavy Metals: Environmental Persistence, Toxicity, and Bioaccumulation

Toxicological perspective on the osmoregulation and ionoregulation physiology of major ions by freshwater animals: Teleost fish, crustacea, aquatic insects, and Mollusca

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