Subchronic inhalation of mixtures of cigarette smoke constituents in Xpa−/−p53+/− knock-out mice: A comparison of intermittent with semi-continuous exposure to acetaldehyde, formaldehyde, and acrolein

Cassee, Flemming R.; Burbure, C.Y. de; Rambali, B; Vleeming, W.; Kuil, A. van de; Steeg, Harry van; Fokkens, Paul; Amsterdam, Jan van; Dormans, J. A. M. A.; Opperhuizen, Antoon

doi:10.1016/j.fct.2007.08.043

Cited by 8 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our study shows that the mRNA and protein expression levels of XPC and ERCC1 in FA-treated BMSCs were significantly higher than those in the control cells, indicating that XPC and ERCC1 may be involved in the repair of human BMSCs DNA damage caused by FA. This is accordance with previous studies [7,39]. The mRNA and protein expression levels of XPA, XPC, and ERCC1 in cells incubated with FA plus APS groups at different concentrations were significantly higher than those in the FA-treated cells.…”

Section: Discussionsupporting

confidence: 93%

Astragalus polysaccharide protects formaldehyde-induced toxicity by promoting NER pathway in bone marrow mesenchymal stem cells

She

Zhao

et al. 2021

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Introduction. In our previous study, it has been confirmed that formaldehyde (FA) not only inhibits the proliferative activity, but also causes DNA-protein crosslinks (DPCs) formation in bone marrow mesenchymal stem cells (BMSCs). The purpose of this study was to detect the protective effect of astragalus polysaccharide (APS) against the cytotoxicity and genotoxicity of BMSCs exposed to FA, and to explore potential molecular mechanisms of APS activity.Material and methods. Human BMSCs were cultured in vitro and randomly divided into control cells (Ctrl group), FA-treated cells (FA group, 120 μmol/L), and cells incubated with FA and increasing concentrations (40, 100, or 400 μg/mL) of APS (FA + APS groups). Cytotoxicity was measured by MTT assay. DNA strand breakage, DNA-protein crosslinks (DPCs), and micronucleus formation were respectively detected by comet assay, KCl-SDS precipitation assay, and micronucleus assay. The mRNA and protein expression level of xeroderma pigmentosum group A (XPA),xeroderma pigmentosum group C (XPC), excision repair cross-complementation group 1 (ERCC1), replication protein A1 (RPA1), and replication protein A2 (RPA2) were all detected by qRT-PCR and Western Blot.Results. Compared with the FA group, the cytotoxicity, DNA strand breakage, DPCs, and micronucleus levels were decreased significantly in FA + APS groups (P < 0.01). Meanwhile, the mRNA and protein expression of XPA, XPC, ERCC1, RPA1, and RPA2 were up-regulated significantly in the FA + APS groups (P < 0.05) with the most prominent effect of the 100 μg/mL APS. Conclusions.Our results suggest that APS can protect the cytotoxicity and genotoxicity of human BMSCs induced by FA. The mechanism may be associated with up-regulated expression of XPA, XPC, ERCC1, RPA1, and RPA2 in the nucleotide excision repair (NER) pathway which promotes DNA damage repair.

show abstract

Section: Discussionsupporting

confidence: 93%

Astragalus polysaccharide protects formaldehyde-induced toxicity by promoting NER pathway in bone marrow mesenchymal stem cells

She

Zhao

et al. 2021

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…As an initial example of the potential impact of these extended airway models, we adapted the nasal CFD/PBPK models developed by Schroeter et al (2008) to evaluated acrolein uptake along all conducting airway walls of our rat, monkey, and human models. As with other reactive, water-soluble aldehydes, acrolein is a respiratory irritant that can produce pathologies in nasal tissues of rodents as well as toxicity to conducting airways at higher exposure concentrations (Cassee et al, 1996(Cassee et al, , 2008Dorman et al, 2008;Lam et al, 1985;Leach et al, 1987). Acrolein is used as an intermediate in the production of acrylic acid and is also formed during the combustion of organic materials (Schroeter et al, 2008).…”

mentioning

confidence: 99%

Comparative Computational Modeling of Airflows and Vapor Dosimetry in the Respiratory Tracts of Rat, Monkey, and Human

Corley

Kabilan

Kuprat

et al. 2012

Toxicological Sciences

159

138

View full text Add to dashboard Cite

Computational fluid dynamics (CFD) models are useful for predicting site-specific dosimetry of airborne materials in the respiratory tract and elucidating the importance of species differences in anatomy, physiology, and breathing patterns. We improved the imaging and model development methods to the point where CFD models for the rat, monkey, and human now encompass airways from the nose or mouth to the lung. A total of 1272, 2172, and 135 pulmonary airways representing 17±7, 19±9, or 9±2 airway generations were included in the rat, monkey and human models, respectively. A CFD/physiologically based pharmacokinetic model previously developed for acrolein was adapted for these anatomically correct extended airway models. Model parameters were obtained from the literature or measured directly. Airflow and acrolein uptake patterns were determined under steady-state inhalation conditions to provide direct comparisons with prior data and nasal-only simulations. Results confirmed that regional uptake was sensitive to airway geometry, airflow rates, acrolein concentrations, air:tissue partition coefficients, tissue thickness, and the maximum rate of metabolism. Nasal extraction efficiencies were predicted to be greatest in the rat, followed by the monkey, and then the human. For both nasal and oral breathing modes in humans, higher uptake rates were predicted for lower tracheobronchial tissues than either the rat or monkey. These extended airway models provide a unique foundation for comparing material transport and site-specific tissue uptake across a significantly greater range of conducting airways in the rat, monkey, and human than prior CFD models.

show abstract

“…After 13 weeks, atrophy of the olfactory epithelium generally appeared, but disappeared after 1 year, while respiratory epithelium metaplasia of the olfactory epithelium occurred at a higher incidence at 1 year. With the exception of a higher incidence of tumors observed in knockout mice than wild-type mice in the semicontinuous aldehyde exposure and controls, no differences between the semicontinuous and intermittent exposure groups were reported (Cassee et al 2008). …”

Section: Mixtures Of Aldehydesmentioning

confidence: 99%

“…Histopathological changes in the nasal cavity and lungs of Xpa À/À p53 þ/À knockout mice were examined following subchronic inhalation of AcH, FA, and Acr (Cassee et al 2008). Mice were exposed either intermittently to aldehydes for 7 min every 45 min, 12 times day À1 , 5 day week À1 or semicontinuously to half-doses of aldehydes for 8 h day À1 , 5 days week À1 .…”

Section: Mixtures Of Aldehydesmentioning

confidence: 99%

Aldehydes

Kuykendall¹

2010

Comprehensive Toxicology

View full text Add to dashboard Cite

Subchronic inhalation of mixtures of cigarette smoke constituents in Xpa−/−p53+/− knock-out mice: A comparison of intermittent with semi-continuous exposure to acetaldehyde, formaldehyde, and acrolein

Cited by 8 publications

References 20 publications

Astragalus polysaccharide protects formaldehyde-induced toxicity by promoting NER pathway in bone marrow mesenchymal stem cells

Astragalus polysaccharide protects formaldehyde-induced toxicity by promoting NER pathway in bone marrow mesenchymal stem cells

Comparative Computational Modeling of Airflows and Vapor Dosimetry in the Respiratory Tracts of Rat, Monkey, and Human

Aldehydes

Contact Info

Product

Resources

About