Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue

Stojanović, Mirjana D.; Todorović, Dušan; Šćepanović, Lj; Mitrović, Dušan; Borozan, Sunčica; Dragutinović, Vesna; Labudović‐Borović, Milica; Krstić, Danijela; Čolović, Mirjana B.; Djuric, Dragan

doi:10.1007/s11010-018-3311-2

Cited by 16 publications

(17 citation statements)

References 45 publications

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“…Our results demonstrated increased levels of MDA concentration in the ileum tissue homogenate, after a three-week methionine treatment, compared to the control group. These results are consistent with our previous research, which included determination of the oxidative stress markers in the colon and liver tisssue homogenates, following methionine application (Stojanović et al 2018a(Stojanović et al , 2018b. These effects could be explained by the prooxidative properties of the methionine.…”

Section: Discussionsupporting

confidence: 93%

“…Our results showed lower amounts of GSH in rats that received methionine i.p, compared to the control group. Therefore, in combination with our previous investigations (Stojanović et al 2018a(Stojanović et al , 2018b, we demonstrated lowering of the reduced glutathione content in the liver and colon tissue homogenates after methionine treatment. We are free to conclude that methionine application compromised antioxidant status in these organs.…”

Section: Discussionsupporting

confidence: 88%

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Effects of subchronic methionine stimulation on oxidative status and morphological changes in the rat ileum

Todorović

Stojanović²,

Šćepanović³

et al. 2019

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This study was conducted to explore the effects of sulfur containing amino acids on redox status and morphological parameters in the rat ileum tissue. Male Wistar albino rats were randomly divided into the following groups: K group (saline, 1 ml/day, i.p.), M group (methionine, 0.8 mmol/ kg/day, i.p.), C group (methionine (0.8 mmol/kg/day) + L-cysteine (7 mg/kg/day), i.p.) and N group (methionine (0.8 mmol/kg/day) + N-acetyl-L-cysteine (50 mg/kg/day), i.p.). Activities of antioxidant enzymes in the ileum were analyzed to profile oxidative status. Morphometric analysis included measurement of villus height (μm), tunica mucosa thickness (μm), tunica muscularis thickness (μm), the total thickness of the ileal wall (μm) and the number of cells in the lamina propria (per 0.1 mm 2 of tissue). Results showed that methionine treatment reduced the activity of antioxidant enzymes (SOD, GPx, CAT) and the GSH content compared to the control group (p > 0.05). The application of methionine reduced the following parameters statistically significant compared to the control group: length of the ileal villi (p < 0.01), tunica mucosa thickness (p < 0.01), and ileal wall thickness (p < 0.01). We concluded that methionine induced the changes in the gut redox status, which implied oxidative stress occurrence. L-cysteine and N-acetyl-L-cysteine both exhibited antioxidant properties.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 88%

Effects of subchronic methionine stimulation on oxidative status and morphological changes in the rat ileum

Todorović

Stojanović²,

Šćepanović³

et al. 2019

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“…More specifically, as shown in Figure 2 , the larger and brighter the node is, the greater the degree of correlation is, which allows for the perception of differentiating the relativity of each node with oxidative hepatotoxicity through the color gradient from bright to dark (i.e., brightest-green; middle-yellow; darkest-red). Noticeably, since the serum aminotransferases of ALT, AST, and ALP are well-accepted indicators for reflecting the severity of liver diseases directly, the measurement of these three targets exists widely in the majority of liver disease studies, which results in the extremely high value of the degree of correlation [ 142 ]. Although we cannot postulate that oxidative hepatotoxicity is not associated with the alteration of ALT, AST, and ALP, these three serum aminotransferases with high correlation values will greatly interfere with the judgment of ranking other targets in relation to oxidative liver injury.…”

Section: Network Pharmacology-associated Studymentioning

confidence: 99%

Molecular Mechanisms Involved in Oxidative Stress-Associated Liver Injury Induced by Chinese Herbal Medicine: An Experimental Evidence-Based Literature Review and Network Pharmacology Study

Zhang

Wang

et al. 2018

IJMS

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Oxidative stress, defined as a disequilibrium between pro-oxidants and antioxidants, can result in histopathological lesions with a broad spectrum, ranging from asymptomatic hepatitis to hepatocellular carcinoma in an orchestrated manner. Although cells are equipped with sophisticated strategies to maintain the redox biology under normal conditions, the abundance of redox-sensitive xenobiotics, such as medicinal ingredients originated from herbs or animals, can dramatically invoke oxidative stress. Growing evidence has documented that the hepatotoxicity can be triggered by traditional Chinese medicine (TCM) during treating various diseases. Meanwhile, TCM-dependent hepatic disorder represents a strong correlation with oxidative stress, especially the persistent accumulation of intracellular reactive oxygen species. Of note, since TCM-derived compounds with their modulated targets are greatly diversified among themselves, it is complicated to elaborate the potential pathological mechanism. In this regard, data mining approaches, including network pharmacology and bioinformatics enrichment analysis have been utilized to scientifically disclose the underlying pathogenesis. Herein, top 10 principal TCM-modulated targets for oxidative hepatotoxicity including superoxide dismutases (SOD), malondialdehyde (MDA), glutathione (GSH), reactive oxygen species (ROS), glutathione peroxidase (GPx), Bax, caspase-3, Bcl-2, nuclear factor (erythroid-derived 2)-like 2 (Nrf2), and nitric oxide (NO) have been identified. Furthermore, hepatic metabolic dysregulation may be the predominant pathological mechanism involved in TCM-induced hepatotoxic impairment.

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“…DL-Met, for 3 weeks (n = 10)]. Acute and subchronic experimental protocols were chosen according to literature data 24,25 .…”

Section: Physiological Assay and Experimental Protocolmentioning

confidence: 99%

The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue

Mićović

Kostic

Mutavdzin

et al. 2020

VSP

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Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1?0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175043]

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Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue

Cited by 16 publications

References 45 publications

Effects of subchronic methionine stimulation on oxidative status and morphological changes in the rat ileum

Effects of subchronic methionine stimulation on oxidative status and morphological changes in the rat ileum

Molecular Mechanisms Involved in Oxidative Stress-Associated Liver Injury Induced by Chinese Herbal Medicine: An Experimental Evidence-Based Literature Review and Network Pharmacology Study

The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue

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