Subclasses of T cells with different sensitivities to cytotoxic antibody in the presence of anesthetics

Lee, S.‐K.; Singh, Jagat N.; Taylor, R. B.

doi:10.1002/eji.1830050408

Cited by 78 publications

(14 citation statements)

References 11 publications

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“…After centrifugation, the PBLs were resuspended with MEM containing BSA (10 mg/ml) and heparin (5 units/ml), and counted. Cell viability was determined using acridine orange ethidium bromide solution (Lee et al, 1975).…”

Section: Methodsmentioning

confidence: 99%

Induction and inhibition of bovine leukaemia virus expression in naturally infected cells

Zandomeni

Carrera-Zandomeni

Esteban

et al. 1992

Journal of General Virology

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Bovine leukaemia virus (BLV) resides in infected lymphocytes in a latent, repressed state but becomes expressed a few hours after the cells are cultured in vitro. We have identified several conditions and factors affecting the expression of BLV in short-term cultures of naturally infected lymphoid cells. The presence of foetal calf serum in the culture medium greatly stimulates virus expression. This stimulation is not due to cellular proliferation. Transcription of BLV RNA and synthesis of p25 in the cultures of peripheral blood lymphocytes are preceded by a lag period of several hours. Synthesis of BLV p25 in these cultures takes place almost immediately after viral RNA synthesis. Extending previous results, we demonstrate that the plasma and lymphatic fluid of cattle contain factors that suppress and stimulate BLV expression. As a result of systematic examination of several parameters, we have developed reproducible assays for the detection of these factors. It is very likely that their relative concentration in the host is an important determinant of susceptibility and resistance to the development of lymphosarcoma and persistent lymphocytosis in BLVinfected cattle.

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Section: Methodsmentioning

confidence: 99%

Induction and inhibition of bovine leukaemia virus expression in naturally infected cells

Zandomeni

Carrera-Zandomeni

Esteban

et al. 1992

Journal of General Virology

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“…Peripheral blood mononuclear cells (PBMCs) were removed from the interface and washed twice in RPMI complete medium supplemented with 2 mmol L-glutamine/ l, 100 mg streptomycin/ml and 100 mg penicillin/ml (Gibco, Grand Island, New York, USA). Cell viability was assessed by fluorescence microscopy using ethidium bromide/acridine orange (Lee et al, 1975), cells were counted and resuspended in complete medium at 2 Â 10 6 cells/ml for both lymphocyte proliferation and cytokine assays.…”

Section: Preparation Of Peripheral Blood Mononuclear Cellsmentioning

confidence: 99%

The effects of conjugated linoleic acid supplementation on immune function in healthy volunteers

et al. 2005

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Objective: To assess the effects of dietary supplementation using two isomeric blends of conjugated linoleic acid (CLA) on immune function in healthy human volunteers. Design: Double-blind, randomised, placebo-controlled intervention trial. Subjects and intervention: A total of 55 healthy volunteers (n ¼ 20 males, n ¼ 35 females) were randomised into one of three study groups who received 3 g/day of a fatty acid blend containing a 50:50 cis-9, trans-11: trans-10, cis-12 CLA isomer blend (2 g CLA), and 80:20 cis-9, trans-11: trans-10, cis-12 (80:20) CLA isomer blend (1.76 g CLA) or linoleic acid (control, 2 g linoleic acid) for 8 weeks.Results: Supplementation with the 80:20 CLA isomer blend significantly (Pr0.05) enhanced PHA-induced lymphocyte proliferation. CLA decreased basal interleukin (IL)-2 secretion (Pr0.01) and increased PHA-induced IL-2 and tumor necrosis factor a (TNFa) production (Pr0.01). However, these effects were not solely attributable to CLA as similar results were observed with linoleic acid. CLA supplementation had no significant effect on peripheral blood mononuclear cells IL-4 production, or on serum-soluble intercellular adhesion molecule-1 (sICAM-1) or plasma prostaglandin E 2 (PGE 2 ) or leukotreine B 4 (LTB 4 ) concentrations.Conclusions: This study shows that CLA supplementation had a minimal effect on the markers of human immune function. Furthermore, supplementation with CLA had no immunological benefit compared with linoleic acid. Sponsorship: CLA supplements were provided by Loders Croklaan.

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“…The number of viable nucleated cells in the synovial fluids of rabbits was estimated using a mixture of acridine orange and ethidium bromide [4], Cells were usually more than 95% viable. The percentage of T cells in synovial fluids was determined by rosetting with papain-treated rabbit erythrocytes as previously described [5].…”

Section: Enumeration O F Nucleated Cells and T Cells In Synovial Fluimentioning

confidence: 99%

Comparison of the Arthritogenic Properties of Dietary Cow’s Milk, Egg Albumin and Soya Milk in Experimental Animals

Welsh

Hanglow

Conn

et al. 1986

Int Arch Allergy Immunol

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The arthritogenic effect of dietary cow’s milk, egg albumin and soya milk were compared in Old English rabbits. The 12-week cow’s milk feeding regimen produced the highest incidence of significant joint lesions. Lesions were evident but mild at 5 weeks and the synovitis had resolved by 32 weeks. It is suggested that the transient nature of the synovitis may be due to the development of specific secretory IgA antibodies which were detectable in faecal pellet extracts. Sandy Lop rabbits were less susceptible to the arthritogenic effect than were Old English rabbits. Dietary ovalbumin was less arthritogenic than cow’s milk despite high titres of serum and synovial fluid antibodies and immune complexes. The rabbits were ‘tolerant’ to dietary soya due probably to pre-existing levels of soya protein in their diet. Lewis and Wistar strain rats, CBA, Balb/c and C57/BL6 mice fed on cow’s milk for 3 months did not develop serum antibodies or synovial lesions. It is suggested that this allergic synovitis is not a model for early rheumatoid joint disease because of the transience of the lesions and lack of stimulation of rheumatoid factor. It may well, however, be a model for the arthralgia seen in patients with certain food allergies.

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Subclasses of T cells with different sensitivities to cytotoxic antibody in the presence of anesthetics

Cited by 78 publications

References 11 publications

Induction and inhibition of bovine leukaemia virus expression in naturally infected cells

Induction and inhibition of bovine leukaemia virus expression in naturally infected cells

The effects of conjugated linoleic acid supplementation on immune function in healthy volunteers

Comparison of the Arthritogenic Properties of Dietary Cow’s Milk, Egg Albumin and Soya Milk in Experimental Animals

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