Subcomponents of Vitamine B Complex Regulate the Growth and Development of Human Brain Derived Cells

Abstract

The work is focused on the role of single components of Vitamin B complex: pyridoxine, riboflavin, thiamine, and nicotinamide in the processes of growth and development of the human brain derived cells. We, also, have assayed the activity of Xanthine Oxidase in the presence of above mentioned subcomponents to delineate the possible mechanism of their action. Results indicate that the all components of Vitamin B complex might be responsible for cells' growth, maturation, proliferation. During early period of the cells' growth the most important components were thiamine and Pyridoxine, initiating cells' proliferation (number of the cells in one field: 2556, 17±355, 68, 2179,0±223,55, resp) vs control (1562,94±146,45), whereas during the late stages of maturation the most important components responsible for differentiation, were riboflavin and nicotinamide (3774.77± 188.41, 3558.82±152.90 resp. vs control 2905±263.75; p<0.035). In comparison with the all other subcomponents of Vitamin B complex only in pyridoxine containing samples, XO activity was specifically inhibited by allopurinol (percentile of inhibition 160,00±60,00 vs control 31,03±6,92, p<0,05). We have concluded that Pyridoxine might interact with XO and regulate its activity. All components of Vitamin B complex are able to initiate cells development and growth; however, they are supposed to be selectively utilized in time dependent manner to guarantee the highest efficiency of the cells development, maturation and proliferation

LSD1 Mediates Neuronal Differentiation of Human Fetal Neural Stem Cells by Controlling the Expression of a Novel Target Gene, HEYL

“…Since mammals are not able to synthesize riboflavin, they must derive it from dietary sources. It has been reported that the addition of riboflavin to culture medium of human fetal brain cells including NSCs promotes cell growth and maturation . Taken together with our finding that S2101 represses hfNSC neurogenesis, this indicates that an adequate supply of riboflavin from mother to fetus is essential for LSD1 activation, and thus, for neurogenesis during brain development.…”

An integrated compositional approach to investigating the full effect of dietary intake on health

“…The few nutritional studies that have investigated the association between vitamin B6 (pyridoxine) and SUA levels have not reported any association between vitamin B6 intake and the risk of hyperuricemia [13] or between the serum levels of vitamin B6 and those of UA [14]. However, the result shown in the example is consistent with experimental evidence showing that vitamin B6 greatly inhibits the activity of xanthine oxidase, the enzyme responsible for the last steps of the purine catabolism that promotes the formation of uric acid [15,16].…”

Alternative to FBS in animal cell culture - An overview and future perspective

“…Moreover, coelomic fluid of earthworms, Eudrilus eugeniae [ 68 ] , Eisenia spp., [ 69 ] and Lumbricus spp. [ 70 ], are rich in riboflavin, which is essential for cellular proliferation [ 71 , 72 ], promotes the neural cell proliferation [ 73 ] and neural stem cell differentiation in cell culture, respectively [ 74 ]. Additionally, CF is used as a supplement in plant tissue culture [ 75 ].…”