Subconjunctival Injection of Low Doses of Mitomycin C: Effects on Fibroblast Proliferation

Abstract

We evaluated the effect of low doses of mitomycin C (MMC) in subconjunctival injection on fibroblast viability. Eighteen rabbits received subconjunctival injections: group 1 was injected with balanced salt solution (BSS), group 2 received MMC 0.025 mg, and group 3 MMC 0.005 mg. Tissue samples were taken 1 h after the injection (conjunctiva, sclera and cornea at 0 and 90 °) and cultured. The average scleral and conjunctival outgrowth in the MMC-treated groups was significantly inferior to the outgrowth of the samples treated with BSS. Subconjunctival injections of MMC (0.025 and 0.005 mg) have an inhibitory effect on conjunctiva at 0 ° and on scleral fibroblast populations.

Antifibrotic role of low-dose mitomycin-c-induced cellular senescence in trabeculectomy models

“…We designed a rabbit trabeculectomy model to examine whether low-dose MMC can induce subconjunctival cellular senescence and assess the outcome of filtering surgery by exposing Tenon's capsule to 0.2 μM MMC. Notably, the concentration used as low-dose MMC here is much lower than that of a previous study [15], which demonstrated that 150 μM MMC can inhibit subconjunctival fibroblast proliferation. Histological examination indicated that low-dose MMC could induce subconjunctival cellular senescence but not apoptosis in our rabbit model.…”

“…Although MMC exerts its antiscarring effect by activating apoptotic signaling pathways in fibroblasts [14], the antifibrosis mechanism in trabeculectomy wound healing is complicated. Some patients who have received filtrating surgery benefit from a low-dose MMC subconjunctival injection [15]. Notably, low-dose MMC induces stress-induced premature senescence (SIPS) in A549 cells [16].…”

Resposta cicatricial corneana em diferentes modalidades de cirurgia refrativa

“…A mitomicina C (MMC) é um quimioterápico de ação sistêmica, o qual foi recentemente introduzido na área oftalmológica. Sua aplicação tópica em casos selecionados têm trazido bons resultados no tratamento de glaucoma, pterígio e até neoplasias intra-epiteliais conjuntivais (59)(60)(61) . O mecanismo de ação da mitomicina C baseia-se em seu efeito citostático, bloqueando a replicação do DNA e RNA, bem como toda síntese protéica celular (60) .…”

“…O mecanismo de ação da mitomicina C baseia-se em seu efeito citostático, bloqueando a replicação do DNA e RNA, bem como toda síntese protéica celular (60) . Como conseqüência, a mitomicina C é capaz de inibir a mitose celular e a proliferação das células epiteliais, estromais e endoteliais, bem como fibroblastos conjuntivais, componentes da cápsula de Tenon (59,61) .…”

Mitomycin C: Biological Effects and Use in Refractive Surgery

“…Through inhibition of the proliferation of corneal epithelial cells, stromal cells, endothelial cells, conjunctival cells, and Tenon capsule fibroblasts, the application of MMC has improved the results of treatments of anterior segment disorders, such as glaucoma, pterygium, and conjunctival and corneal intraepithelial neoplasia. [20][21][22][23][24][25] …”