Subcritical Fluid Extraction of Stevia Sweeteners from Stevia rebaudiana

Liu, J.; Ong, Chye Peng; Li, S.F.Y.

doi:10.1093/chromsci/35.9.446

Cited by 26 publications

(16 citation statements)

References 14 publications

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“…Various methods can be adopted to extract the sweetening substances from the leaves (Hashimoto et al, 1978;Kasai et al, 1987;Ahmed and Dobberstein, 1982;Kinghorn et al, 1982;Kinghorn et al, 1984;Little and Stahel, 1984;Kitada et al, 1989;Fullas and Chiang, 1989;Fuh and Chiang, 1990;Liu et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Agronomic-productive characteristics of two genotype of stevia rebaudiana in Central Italy

Andolfi¹,

Macchia²,

Ceccarini³

2006

Ital J Agronomy

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Stevia rebaudiana Bertoni produces a variety of high-potency low calorie sweeteners in its' leaf tissue. The aim of this work was to evaluate the productive potential of two different Stevia rebaudiana genotypes and the characteristics of the production of the plant obtained through different cultivation methods, in central Italy. For several years (1992)(1993)(1994)(1995)(1996)(1997)(1998)(1999)(2000) agronomic trials on Stevia rebaudiana cultivated in the littoral area near Pisa (Italy) carried out. In 1992 two different genotypes of Stevia (B1 and B2) transplanted in silt-loam soil; in 1996 other plants from B2 genotype, produced by vegetative process (micropropagation), were transplanted in the same field. In the period from 1992-1999 the production of leaves from both genotypes increase as of the third year and the ratio leaf-aerial plant, after the peak in the third year, tends to diminish. Except the first year (1997) there were not recorded statistically significant differences between the two propagation methods employed in relation to leaf production. The quantity of leaves produced from a single harvest was less than resulting from two cuttings and the micropropagation plants produced a larger amount of leaves than those from cutting. The leaf-stem ratio was to become an interesting morphological and production characteristic parameter of the plant. Stevia rebaudiana appears particularly suited for the cultivation environment of central Italy. A particular positive aspect that must be considered in these regions is that Stevia can be grown successfully as poliannual species because crop survival over the winter is high. The results obtained show that this species is economically profitable until the 5th or 6th year of cultivation. At our latitudes is also necessary to establish a program of genetic improvement in order to develop earlier varieties that can guaranty an optimum qualitative and quantitative seed production.

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Section: Introductionmentioning

confidence: 99%

Agronomic-productive characteristics of two genotype of stevia rebaudiana in Central Italy

Andolfi¹,

Macchia²,

Ceccarini³

2006

Ital J Agronomy

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“…Water extraction Abou et al, 2010;Inamake et al, 2010;Rao et al, 2012;Afandi et al, 2013;Deshmukh and Kedari, 2014;Gonzalez et al, 2014 Methanol extraction Liu et al, 1997;Pol et al, 2007;Deshmukh andKedari, 2014 Ethanol extraction Erkucuk et al, 2009;Deshmukh and Kedari, 2014 Pressurized fluid extraction (PFE) Pol et al, 2007 Microwave-assisted extraction (MAE) Jataik et al, 2009;Teo et al, 2009;Javad et al, 2014 Ultrasonic-assisted Extraction (UAE) Zlabur et al, 2015 Pressurized hot water extraction(PHWE) Teo et al, 2009;Tiwari et al, 2014 Supercritical fluid extraction (SFE) Pasquel et al, 2000;Choi et al, 2002;Yoda et al, 2003;Pol et al, 2007;Erkucuk et al, 2009Enzymatic extraction Puri et al, 2012Rao et al, 2015 Kolb et al, 2001;Woelwer et al, 2010;Hoekstra et al, 2009;Kumari et al, 2016HPLC C18 Vanek et al, 2001Abou et al, 2010;Afandi et al, 2013;Gonzalez et al, 2015;Gonzalez et al, 2014;Kubica et al, 2015;Samah et al, 2013;Javad et al, (Woelwer, 2012).…”

Section: Extraction Methods Reference(s)mentioning

confidence: 99%

“…On the other hand, Abou et al (2010) found that stevia sweeteners were highly soluble in methanol and less soluble in water. On this basis, Liu et al (1997) extracted stevioside from S. rebaudiana leaves with hot methanol. Pol et al (2007) used pressurized fluid extraction method for the extraction of stevioside from S. rebaudiana leaves using methanol and water.…”

Section: Major Sweet Compoundsmentioning

confidence: 99%

Chemistry and analytical techniques for ent-kaurene-glycosides of Stevia rebaudiana Bertoni - A review

Kumari

Kumar

2017

JANS

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Abstract:The Stevia genus encompasses about 200 herbs and shrubs species. Stevia rebaudiana, one of the members has gained commercial importance as a natural low-calorie sweetener, due to the presence of high concentration of stevioside and rebaudioside -A (25% to 45% of stevioside content) in the leaves. The major processes involved in the production and quantification of steviol glycosides are extraction, purification and estimation. Various extraction methods have been used for extraction of steviol glycosides in the world. The extraction methods of steviol glycosides mostly differed at the stage of clarification of extracts. The present study is an attempt to summarize the scattered literature and reports on a single podium. Moreover, it also depicts upto date literature regarding numerous extraction, purification and quantitative estimation methods for steviol glycosides

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“…Samples of a stevioside solution or methanol extract of leaves of Stevia rebaudiana (Sigma, St Louis, MO, USA) prepared as described in the literature [6,7,11] were injected onto the HPLC system.…”

Section: Methodsmentioning

confidence: 99%

Determination of stevioside by high-performance liquid chromatography with pulsed amperometric detection

Ahmed

Smith²

2002

J. Sep. Science

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Subcritical Fluid Extraction of Stevia Sweeteners from Stevia rebaudiana

Cited by 26 publications

References 14 publications

Agronomic-productive characteristics of two genotype of stevia rebaudiana in Central Italy

Agronomic-productive characteristics of two genotype of stevia rebaudiana in Central Italy

Chemistry and analytical techniques for ent-kaurene-glycosides of Stevia rebaudiana Bertoni - A review

Determination of stevioside by high-performance liquid chromatography with pulsed amperometric detection

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