Subcritical n-hexane/isopropanol extraction of lipid from wet microalgal pastes of Scenedesmus obliquus

Bian, Xiaoyu; Jin, Wei; Qiong, GU; Zhou, Xu; Xi, Yuhe; Tu, Renjie; Han, Song-Fang; Xie, Guo-Jun; Gao, Shuhong; Wang, Qilin

doi:10.1007/s11274-018-2421-z

Cited by 18 publications

(9 citation statements)

References 28 publications

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“…All BCFA used in this study were extracted from anchovies (Zhougang Aquatic Products Co., Ltd, Zhoushan, China) using a method described by Bian et al 18 . Anchovies were dried and processed into powder in a crusher.…”

Section: Methodsmentioning

confidence: 99%

“…All BCFA used in this study were extracted from anchovies (Zhougang Aquatic Products Co., Ltd, Zhoushan, China) using a method described by Bian et al 18 Anchovies were dried and processed into powder in a crusher. A solution of N-hexane and isopropanol (3:2 v/v) in combination with ultrasonic (JY92-IIN, Ningbo Scientz Biotechnology Co., Ltd, Ningbo, China) assistance (500 W, 20 min) was used to extract BCFA.…”

Section: Preparation Of Bcfamentioning

confidence: 99%

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Goat milk powder supplemented with branched‐chain fatty acid: influence on quality and microstructure

et al. 2022

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BACKGROUND: Branched-chain fatty acid (BCFA) is effective in preventing and helping to treat neonatal necrotizing enterocolitis. It is essential to supplement goat-milk powder for formula-fed preterm infants with BCFA. In this study, the quality and microstructures of milk powders supplemented with different concentrations of BCFA were evaluated, using goat milk powder without BCFA as the control group (CG).RESULTS: In comparison with the CG, goat milk powder supplemented with BCFA exhibited smaller fat globules and a significant drop in overall particle size. During 16 weeks of storage, BCFA-supplemented groups showed suitable moisture content and viscosity and good solubility. The BCFA also helped reduce the number of folds on the surface of the milk powder particles. CONCLUSION:The findings of this study indicate that goat milk powders with BCFA exhibit differences in quality and microstructure in comparison with ordinary goat milk powder, which is relevant for the future development and application of BCFA in foods.

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Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Bcfamentioning

confidence: 99%

Goat milk powder supplemented with branched‐chain fatty acid: influence on quality and microstructure

et al. 2022

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“…The ammonium nitrogen content was measured by distillation in the presence of MgO and collection of the product in a solution 2% H 3 BO 3 in presence of indicator methyl red, and subsequently titration of the product with 0.1 N HCl. The total lipid content was determined with extraction using co-solvents of n-hexane/isopropanol in the microalgal biomass according with the method of Bian et al (2018). The solvent ratio of n-hexane to isopropanol was 3/2 (V/V), the ratio of co-solvents to microalgal dry biomass was 10/1 (V/W).…”

Section: Methods Of Analysesmentioning

confidence: 99%

Effect of Macronutrients and of Anaerobic Digestrate on The Heterotrophic Cultivation of Chlorella Vulgaris Grown With Biodiesel By-Products

Gougoulias

Papapolymerou

Mpesios

et al. 2021

Preprint

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The aim of this work was to investigate the kinetics of the heterotrophic growth of Chlorella vulgaris as a means of producing bio-oil for biodiesel production. Glycerol was used as the sole organic carbon substrate. Anaerobic digestrate from a local plant was used to examine its effect on the kinetics and the protein and lipid content of the biomass. The effect of the initial carbon and nitrogen concentrations on the carbon uptake rate was studied independently. In one set of five experiments the organic carbon in the form of glycerol, varied from 0.27 g L-1 to 5.36 g L-1 while the concentration of atomic nitrogen was held constant and equal to 45.4 mg L-1. The Co/No ratio varied from 6 to 118.1. In a second set, also of five experiments, the organic carbon was held constant and equal to 3.3 g L-1 and atomic nitrogen varied from 22.7 mg L-1 to 450 mg L-1. The Co/No ratio varied from 7.3 to 145.4. In a third set of experiments anaerobic digestrate was added in increasing amounts into the culture media from 4% to 16%. It was found that the carbon uptake rate as well as the lipid and protein content depended on the Co/No ratio. Increasing ratios of Co/No lead to higher carbon uptake rates, higher lipid content and lower protein content. The initial nitrogen concentration was also found to affect the growth rate of C. vulgaris. The addition of anaerobic digestrate did not affect appreciably the protein and lipid content of the biomass while, addition of anaerobic digestrate up to 16% in the culture medium increased the carbon uptake rate up to about 24%.

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“…Gigliotti et al [13] tested acetone/ethanol mixtures for the lipid extraction from Antarctic krill (Euphausia superba). Bian et al [14] maximized the lipid and fatty acid (FA) yield from the microalga Scenedesmus obliquus by using subcritical n-hexane/isopropanol extraction. Li et al [15] tested different combinations of low-toxicity solvents for obtaining antioxidants (i.e., vitamin E) and edible oils from the microalga Scenedesmus dimorphus.…”

Section: Introductionmentioning

confidence: 99%

Low-Toxicity Solvents for the Extraction of Valuable Lipid Compounds from Octopus (Octopus vulgaris) Waste

Méndez,

Rodríguez,

Aubourg

et al. 2023

Foods

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This study focused on the recovery of valuable lipid compounds from octopus (Octopus vulgaris) by-products. Extraction conditions of total lipids (TLs), phospholipids (PLs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) were optimized by employing a Simplex-Lattice design; for it, different relative concentrations of three low-toxicity solvents (ethanol, acetone, and ethyl acetate) were considered. The optimization process was also addressed in reference to fatty acid (FA) ratios (total polyunsaturated FAs/total saturated FAs and total ω3 FAs/total ω6 FAs). The variance analysis of multiple regression data demonstrated that the quadratic model was significant (p < 0.05) for TL, PL, and DHA values and the ω3/ω6 ratio. As a result, the following optimized values were obtained: 113.8 g·kg−1 dry by-products (TLs), 217.3 g·kg−1 lipids (PLs), 22.55 g·100 g−1 total FAs (DHA), and 3.70 (ω3/ω6 ratio). According to the model developed, optimized values were shown to correspond to the following relative solvent concentrations (ethanol/acetone/ethyl acetate): 0.46/0.00/0.54, 0.93/0.07/0.00, 0.83/0.17/0.00, and 0.64/0.00/0.36, respectively. Comparison to yields obtained by the conventional chloroform/methanol method was carried out. A novel strategy based on the employment of low-toxicity solvents is proposed for the extraction of valuable lipid constituents from octopus waste. A different solvent ratio would be necessary according to the lipid compound concerned.

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Subcritical n-hexane/isopropanol extraction of lipid from wet microalgal pastes of Scenedesmus obliquus

Cited by 18 publications

References 28 publications

Goat milk powder supplemented with branched‐chain fatty acid: influence on quality and microstructure

Goat milk powder supplemented with branched‐chain fatty acid: influence on quality and microstructure

Effect of Macronutrients and of Anaerobic Digestrate on The Heterotrophic Cultivation of Chlorella Vulgaris Grown With Biodiesel By-Products

Low-Toxicity Solvents for the Extraction of Valuable Lipid Compounds from Octopus (Octopus vulgaris) Waste

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