Subcritical solvent extraction of total anthocyanins from dried purple waxy corn: Influence of process conditions

Muangrat, Rattana; Williams, Paul T.; Saengcharoenrat, Phanat

doi:10.1111/jfpp.13252

Cited by 22 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They found that the mean DPPH IC 50 value of purple corn cob extract was 40.1 μg/ml. Also Muangrat et al () reported the DPPH IC 50 value of 3.50 mg/ml for a dried cob extract obtained from purple waxy corn ( genotype Fancy Muang 111 ) when the dried cob was extracted under subcritical solvent extraction using water–ethanol at ratio 1:1 as solvent and sample–solvent at ratio 1:20 at 100 °C for 15 min with N 2 purging.…”

Section: Resultsmentioning

confidence: 99%

“…The stable organic free radical DPPH can be scavenged when encounter a proton donating substance such as an antioxidant, in order to reduce its absorbance. The scavenging activity of natural extracts produced from fruit, vegetable, or agro‐industrial wastes, can be detected via DPPH assay (Muangrat, Williams, & Saengcharoenrat, ; Ramos‐Escudero, Munoz, Alvarado‐Ortız, Alvarado, & Yanez, ; Sánchez‐Moreno, ; Yang & Zhai, , ). Additionally, the DPPH assay can accommodate many extract samples in a short period and is sensitive to detect active ingredients even at low concentrations.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Ultrasound assisted extraction of anthocyanins and total phenolic compounds from dried cob of purple waxy corn using response surface methodology

Muangrat

Pongsirikul

Blanco

2017

J Food Process Preserv

Self Cite

View full text Add to dashboard Cite

Ultrasound‐assisted extraction of anthocyanins and total phenolic compounds from the dried cob of purple waxy corn was investigated using response surface methodology, where the process variables were ultrasound amplitude levels (25, 50, 100%) and different solvents (pure water, water–ethanol ratio 1:1, pure ethanol), using a sample–solvent ratio of 1:20, and extraction temperature of 65 °C for 30 min. Central composite face‐centered design was used to determine optimum process conditions. The results showed that the overall optimal condition for simultaneous extraction of anthocyanins and phenolic compounds was obtained by using 50% water in water–ethanol mixed solvent as solvent and amplitude level of 50%. The optimal yields of anthocyanins and phenolic compounds were 240.161 μg cyanidin‐3‐glucoside/g dry sample and 27.662 mg gallic acid/g dry sample, respectively. The result for the antioxidant activity of the extract obtained from this optimal extraction condition to decrease the initial DPPH• concentration by 50% was approximately 4.64 mg/ml. Practical applications This article has practical application to study extraction of total anthocyanins and total phenolic compounds from dried cob of purple waxy corn using ultrasound‐assisted extraction. The response surface method using central composite face‐centered design was successfully employed to predict the effect of two independent variables on the ultrasound assisted extraction of total anthocyanins and total phenolic compounds. Moreover, the methodology and results presented in this study could be beneficial for future efforts to increase the recovery of potentially bioactive compounds from fruits, vegetables, plants and agricultural residues.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Ultrasound assisted extraction of anthocyanins and total phenolic compounds from dried cob of purple waxy corn using response surface methodology

Muangrat

Pongsirikul

Blanco

2017

J Food Process Preserv

Self Cite

View full text Add to dashboard Cite

show abstract

“…PWC cobs and seeds were ground in an electrical herb grinder separately, while boiled seed was steamed for 30 min before grinding and then extracted using either deionized water (DI water) or 70 % acetone as solvents with the sample:solvent ration of 25 g:1000 ml under 60º for 4 h. The extracts were filtered by Whatman no.1 filter paper to remove solid residual. About 0.1 % w/w ascorbic acid was added to protect oxidation before removing water by freezedrying at -30º under pressure 130 mbar for 72 h. The dried extracts were preserved at -20º [19,20] .…”

Section: Purple Corn Extractionmentioning

confidence: 99%

“…Antioxidant activity also determined by ferric reducing/antioxidant power (FRAP) assay. The antioxidant activity evaluated by FRAP was calculated as trolox equivalent (µM trolox/mg plant extract) [19,25] .…”

Section: Determination Of Bioactive Compounds and Antioxidant Activitymentioning

confidence: 99%

Protective Effects of Purple Waxy Corn on Aflatoxin B1-induced Oxidative Stress and Micronucleus in HepG2 Cells

Singto¹,

Tassaneeyakul²,

Porasuphatana³

2020

Indian J Pharm Sci

View full text Add to dashboard Cite

The purpose of this study was to investigate the protective effects of purple waxy corn extract (Zea may L.) on aflatoxin B1-induced cytotoxicity, oxidative stress and micronuclei in HepG2 cells. Purple waxy corn extract contained phenolics (187.15-341.65 µg gallic acid/mg), flavonoids (0.55-21.35 µg quercetin/ mg) and anthocyanins (43.70-220.43 mg cyanidin-3-glucoside/l). Antioxidant activities of the extracts were measured using oxygen radical absorbance capacity (1410.70-2214.49 µM trolox/mg) and ferric reducing/ antioxidant power (292.38-612.38 µM trolox/mg) assays. There were significant correlations between phenolic, flavonoid and anthocyanin content and antioxidant activities (R=0.740-0.778) of purple waxy corn extract. Studies in HepG2 cells found that 0.5 and 1 mg/ml purple waxy corn extract increased cell viability in AFB1-treated HepG2 cells. Purple waxy corn extract reduced intracellular ROS production, increased GSH content and antioxidant enzyme GR, GPx and GST activities and micronucleus in aflatoxin B1-treated HepG2 cells. Therefore, purple waxy corn extract reduced aflatoxin B1 toxicity through induction of antioxidant activities and, thus, inhibited oxidative stress-induced DNA damage caused by aflatoxin B1s.

show abstract

“…Anthocyanins – water‐soluble polyphenol flavonoids – are often used as natural pigments in food production and processing, and they possess antioxidation, anti‐aging and anticancer properties, as well as providing liver and eyesight protection 5,6 . Moreover, PCC extract has been shown to have excellent antioxidant activity and inhibit C6 glioma cell proliferation in vitro 7,8 . It is of great significance to further develop the medicinal value of the waste purple corncobs.…”

Section: Introductionmentioning

confidence: 99%

Purified anthocyanins from Zea mays L. cob ameliorates chronic liver injury in mice via modulating of oxidative stress and apoptosis

et al. 2021

View full text Add to dashboard Cite

BACKGROUND Purple corn (Zea mays L.) is one of the main economic crops in China and has been used in the treatment of cystitis, urinary infections and obesity. However, purple corncobs, the by‐product remaining after processing and having an intense purple‐black color, are normally disposed of as waste or used as animal feed. Therefore, to further expand the medicinal value of purple corncob, its content was analyzed and, after purification, the effect and mechanism of purified purple corncob anthocyanins (PPCCA) on CCl4‐induced chronic liver injury in mice were investigated. RESULTS It was observed that the total anthocyanin content (TAC) from PPCCA (317.51 ± 9.30 mg cyanidin 3‐O‐glucoside (C‐3‐G) g−1 dry weight) was significantly higher than that from the purified purple corn seed anthocyanin (266.73 ± 3.67 mg C‐3‐G g−1 dry weight), of which C‐3‐G accounted for 90.6% and 90.4% of the TAC, respectively. Furthermore, compared with the CCl4 group, PPCCA treatment significantly reduced liver index, serum total bilirubin, alanine transaminase, aspartate transaminase and liver malondialdehyde levels, but increased liver superoxide dismutase activity. The pathological changes were also improved, such as more regular arrangement of hepatocytes, less swelling, and fewer vacuoles and apoptotic cells. Additionally, mechanistic studies showed that PPCCA downregulated the expression of Caspase‐3, Bax and cytochrome P450 2E1 proteins in the liver and upregulated the expression of Bcl‐2. CONCLUSION These results demonstrated that PPCCA could ameliorate CCl4‐induced chronic liver injury by regulating oxidative stress and hepatocyte apoptosis pathways. © 2021 Society of Chemical Industry

show abstract

Subcritical solvent extraction of total anthocyanins from dried purple waxy corn: Influence of process conditions

Cited by 22 publications

References 21 publications

Ultrasound assisted extraction of anthocyanins and total phenolic compounds from dried cob of purple waxy corn using response surface methodology

Ultrasound assisted extraction of anthocyanins and total phenolic compounds from dried cob of purple waxy corn using response surface methodology

Protective Effects of Purple Waxy Corn on Aflatoxin B1-induced Oxidative Stress and Micronucleus in HepG2 Cells

Purified anthocyanins from Zea mays L. cob ameliorates chronic liver injury in mice via modulating of oxidative stress and apoptosis

Contact Info

Product

Resources

About