Subcritical water extraction of betulinic acid from birch bark

Liu, Jing; Pan, Changtian; Yao, Wenjia; Wang, Jia; Wang, Luying; Deng, Lihong; He, Jing; Zhang, Guifeng; Lei, Jiandu

doi:10.1016/j.indcrop.2015.05.064

Cited by 29 publications

(15 citation statements)

References 32 publications

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“…The critical point of water is at 22.4 MPa and 374°C and water below these critical points has demonstrated its ability to extract different classes of compounds depending on pressure and temperature programming (Duba et al, 2015;Liu et al, 2015). At subcritical condition, more polar compounds are extracted at low temperature while the less polar compounds are extracted at higher temperatures into subcritical water (Luong et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Subcritical water extraction of bioactive compounds from dry loquat (Eriobotrya japonica) leaves and characterization of triterpenes in the extracts

Mlyuka¹,

Zhang²,

Zheng³

et al. 2016

Afr. J. Biotechnol.

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Medicinal properties of loquat leaf extracts (LLEs) are associated with their constituents of phenolic compounds and triterpenes. In this study, the efficacy of subcritical water extraction (SWE) technique was assessed by comparing with conventional solid-liquid extraction (CE) and Soxhlet extraction (SE). Results showed that the highest yields of total polyphenols were 82.7 ± 1.5 mgGAE/g leaf weight (LW), total flavonoids (54.1 ± 4.1 mgQE/g LW) and total triterpenoids (37.5 ± 3.2 mgUAE/g LW) were obtained by SWE compared to total polyphenols (61.8 ± 3.3 mgGAE/g LW), total flavonoids (43.2 ± 0.6 mgQE/g LW) and total triterpenoids (28.7 ± 2.3 mgUAE/g LW) extracted by SE and total polyphenols (50.3 ± 1.8 mgGAE/g LW), total flavonoids (40.4 ± 2.1 mgQE/g LW) and total triterpenoids (22.9 ± 3.2 mgUAE/g LW) obtained by CE. The extraction efficiency of triterpenes using SWE was about 1.7 times higher than those obtained using traditional extraction methods, and their main structural pattern of the cured extracts was comparable to the extracts obtained using traditional extraction methods.The infrared spectra obtained from the three extraction techniques appeared identical, but the variation in the intensity of the peak of absorption was visible among the three extraction techniques. The similarity of the infrared spectral pattern (peak coincided peak by peak) implies that the triterpenes in the extract obtained by the three techniques were identical by LC/MS. The findings of this study have demonstrated that SWE can be employed as an alternative green extraction technology to get important phytochemicals from plant sources.

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Section: Introductionmentioning

confidence: 99%

Subcritical water extraction of bioactive compounds from dry loquat (Eriobotrya japonica) leaves and characterization of triterpenes in the extracts

Mlyuka¹,

Zhang²,

Zheng³

et al. 2016

Afr. J. Biotechnol.

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“…This phenomenon was due to the fact that water at a higher temperature had a lower dielectric constant, reducing the energy required for division in solute-matrix interactions, and also reduced polarity due to weakened hydrogen bonds [30]. Furthermore, water in its subcritical state exhibited hydrolytic properties as its ionization constant increased, producing an increase in the RES extraction efficiency as temperature increased [31]. Beyond an extraction time of 24.89 min or extraction temperature of 152.32 • C, there was no further increase in the RES yield.…”

Section: Optimization Of Subcritical Water Extractionmentioning

confidence: 99%

Optimization of Subcritical Water Extraction of Resveratrol from Grape Seeds by Response Surface Methodology

et al. 2017

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Abstract:The subcritical water extraction (SWE) is a high-efficiency and environment-friendly extraction method. The extraction of resveratrol (RES) of grape seeds obtained from the wine production process was proposed using subcritical water extraction (SWE). The effects of different extraction process parameters on RES yield were investigated by single factors. Extraction optimization was conducted using response surface methodology (RSM). Extraction temperature was proven to be the most significant factor influencing RES yield. The optimal conditions was as follows: extraction pressure of 1.02 MPa, temperature of 152.32 • C, time of 24.89 min, and a solid/solvent ratio of 1:15 g/mL. Under these optimal conditions, the predicted extraction RES yield was 6.90 µg/g and the recoveries was up to 91.98%. Compared to other previous studies, this method required less pollution and less treatment time to extract RES from grape seeds. From these results, added economic value to this agroindustrial residue is proposed using environmentally friendly extraction techniques.

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“…Sergey et al developed a four-step processing technique to obtain a betulin yield of 51% from birch (Betula pendula) bark [10]. Recently, subcritical water extraction for betulinic acid from birch bark has been proposed, and 2.803 mg/g birch bark of betulinic acid was achieved under the optimal conditions [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…On the one hand, ethanol is relatively less toxic than acetone and methanol. On the other hand, the ethanol extraction method is more cost-effective in comparison with subcritical/supercritical extraction, because the latter approaches are always undertaken under high pressure [11,12]. However, there is limited information on the toxicity and antioxidant activities of betulin extracts from the bark of Betula platyphylla Suk.…”

Section: Introductionmentioning

confidence: 99%

Parameter Optimization and Potential Bioactivity Evaluation of a Betulin Extract from White Birch Bark

Chen

Han

Pang

2020

Plants

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Owing to its pharmacological potential, betulin has attracted substantial attention in the past two decades. The present work attempts to extract betulin from Betula platyphylla Suk. bark by the ultrasonic-assisted ethanol method and to evaluate its potential bioactivities. The critical process variables affecting the yield were optimized by a four-factor, three-level, central composite response surface methodology (RSM). A betulin yield of 92.67% was achieved under the optimum conditions: 65% ethanol concentration, 1:25 ratio of white birch bark to solvent, an extraction temperature of 30 °C, and an extraction time of 30 min. The ratio of solid to solvent is the most significant parameter in terms of yield. The optimal conditions were validated through experiments, and the observed value (92.67 ± 2.3%) was interrelated with the predicted value (92.86 ± 1.5%). The betulin extract was analyzed quantitatively by HPLC and quantitatively by LC/MS, before its potential biological activities were evaluated. Bioactivity surveys confirmed that the betulin extract showed not only no embryo deformity through zebrafish administration experiments, but also no cytotoxicity through MTT assays. Furthermore, the betulin extract had strong antioxidant activities in vitro by scavenging ferric reducing power (FRAP), 1,1-diphenyl-2-picryl hydrazyl(DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and chelating metal ions. This study demonstrates that ultrasonic-assisted ethanol extraction may be a green, efficient method for the extraction of betulin from white birch bark, and that betulin extracts are potentially useful in cosmetics, food supplements, or pharmaceutical applications.

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Subcritical water extraction of betulinic acid from birch bark

Cited by 29 publications

References 32 publications

Subcritical water extraction of bioactive compounds from dry loquat (Eriobotrya japonica) leaves and characterization of triterpenes in the extracts

Subcritical water extraction of bioactive compounds from dry loquat (Eriobotrya japonica) leaves and characterization of triterpenes in the extracts

Optimization of Subcritical Water Extraction of Resveratrol from Grape Seeds by Response Surface Methodology

Parameter Optimization and Potential Bioactivity Evaluation of a Betulin Extract from White Birch Bark

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