Subcritical water-hydrolyzed fish collagen ameliorates survival of endotoxemic mice by inhibiting HMGB1 release in a HO-1-dependent manner

Ahn, Min Young; Hwang, Jae Joon; Ham, Sun Ah; Hur, Joon; Jo, Yeon‐Ji; Lee, Sang Yoon; Choi, Mi‐Jung; Han, Sung Gu; Seo, Han Geuk

doi:10.1016/j.biopha.2017.07.041

Cited by 11 publications

(3 citation statements)

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“…NO accumulated in culture medium was determined by measuring the levels of nitrite (an oxidized form of NO) as described previously (Ahn, Hwang, Ham, et al., 2017). Briefly, 100‐μl aliquot of conditioned culture medium was mixed with 100 μl of Griess reagent (equal part of 0.1% N‐(1‐naphthyl)‐ethylenediamine dihydrochloride and 1% sulfanilamide) and incubated for 10 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, formononetin, a herbal isoflavonoid, inhibited LPS‐triggered release of HMGB1 by upregulating SIRT1 (sirtuin 1; silent mating type information regulation 2 homologue 1) in a peroxisome proliferator‐activated receptor (PPAR)δ‐dependent manner (Hwang et al., 2018). In addition, fish collagen hydrolyzed by subcritical water also improved the survival of endotoxemic mice by transcriptional induction of heme oxygenase‐1 (HO‐1) in a nuclear factor erythroid 2‐related factor 2 (Nrf 2) signaling, thereby inhibiting HMGB1 secretion into the circulation (Ahn, Hwang, Ham, et al., 2017). In particular, JAK/STAT1 (Janus kinase/signal transducer and activator of transcription 1) signaling plays a pivotal role in the HMGB1 translocation from nucleus to cytoplasm through promotion of HMGB1 hyperacetylation in LPS‐treated cells (Lu et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

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Ginseng leaf extract ameliorates the survival of endotoxemic mice by inhibiting the release of high mobility group box 1

et al. 2021

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High mobility group box 1 (HMGB1) is a well‐defined mediator involved in the pathophysiologic response to endotoxemia and sepsis. However, the mechanisms and therapeutic agents that could prevent its release are not fully elucidated. Here, the present study demonstrates that the ginseng leaf extract (GLE) regulates lipopolysaccharide (LPS)‐triggered release of HMGB1 in macrophages and endotoxemic animal model. Treatment of RAW264.7 macrophages with GLE significantly inhibited the release of HMGB1 stimulated by LPS. GLE also suppressed the generation of nitric oxide (NO) and expression of inducible NO synthase (iNOS) in a dose‐dependent manner. These effects of GLE were accompanied by inhibition of HMGB1 release stimulated by LPS, indicating a potential mechanism by which GLE regulates HMGB1 release through NO signaling. Furthermore, induction of suppressor of cytokine signaling 1 by GLE‐mediated GLE‐dependent suppression of HMGB1 release and NO/iNOS induction by inhibiting Janus kinase 2/signal transducer and activator of transcription 1 signal in RAW 264.7 cells exposed to LPS. Finally, administration of the GLE ameliorated the survival rate of LPS‐injected endotoxemic mice in a NO‐dependent manner. Thus, GLE may block the LPS‐stimulated release of HMGB1 by regulating cellular signal networks, thereby providing a therapeutic strategy for endotoxemia as a functional food. Practical applications High mobility group box 1 (HMGB1) is released into the extracellular milieu when immune cells are exposed to pathogen‐related molecules such as lipopolysaccharide (LPS), in which it acts as a critical mediator of lethality in sepsis and endotoxemia. The extract of ginseng leaf, which is a part that can be easily thrown away, ameliorated the survival rate of endotoxemic mice by inhibiting HMGB1 secretion in a NO‐dependent manner. Thus, this study suggests that ginseng leaf can be used as a functional food by resolving the immune responses in the pathology of endotoxemia.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ginseng leaf extract ameliorates the survival of endotoxemic mice by inhibiting the release of high mobility group box 1

et al. 2021

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“…M2 macrophages produce anti-inflammatory mediators such as heme oxygenase-1 (HO-1) and NADPH dehydrogenase quinone 1 (NQO1). HO-1, which maintains cellular redox homeostasis, is expressed at a low levels in unstimulated normal cells [8,9]. However, HO-1 expression is increased by lipopolysaccharides (LPS), oxidative stress, pro-inflammatory cytokines, and transforming growth factor-β1 (TGF-β1) and protects cells from oxidative stress and inflammation [10,11].…”

Section: Introductionmentioning

confidence: 99%

Impressic Acid Attenuates the Lipopolysaccharide-Induced Inflammatory Response by Activating the AMPK/GSK3β/Nrf2 Axis in RAW264.7 Macrophages

Lee

Kim

Jin

et al. 2021

IJMS

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Inflammatory diseases are caused by excessive inflammation from pro-inflammatory mediators and cytokines produced by macrophages. The Nrf2 signaling pathway protects against inflammatory diseases by inhibiting excessive inflammation via the regulation of antioxidant enzymes, including HO-1 and NQO1. We investigated the anti-inflammatory effect of impressic acid (IPA) isolated from Acanthopanax koreanum on the lipopolysaccharide (LPS)-induced inflammation and the underlying molecular mechanisms in RAW264.7 cells. IPA attenuated the LPS-induced production of pro-inflammatory cytokines and reactive oxygen species, and the activation of the NF-κB signaling pathway. IPA also increased the protein levels of Nrf2, HO-1, and NQO1 by phosphorylating CaMKKβ, AMPK, and GSK3β. Furthermore, ML385, an Nrf2 inhibitor, reversed the inhibitory effect of IPA on LPS-induced production of pro-inflammatory cytokines in RAW264.7 cells. Therefore, IPA exerts an anti-inflammatory effect via the AMPK/GSK3β/Nrf2 signaling pathway in macrophages. Taken together, the findings suggest that IPA has preventive potential for inflammation-related diseases.

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Collagen

Olatunji¹

2020

Springer Series on Polymer and Composite Materials

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Subcritical water-hydrolyzed fish collagen ameliorates survival of endotoxemic mice by inhibiting HMGB1 release in a HO-1-dependent manner

Cited by 11 publications

References 37 publications

Ginseng leaf extract ameliorates the survival of endotoxemic mice by inhibiting the release of high mobility group box 1

Ginseng leaf extract ameliorates the survival of endotoxemic mice by inhibiting the release of high mobility group box 1

Impressic Acid Attenuates the Lipopolysaccharide-Induced Inflammatory Response by Activating the AMPK/GSK3β/Nrf2 Axis in RAW264.7 Macrophages

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