Subcultured Odontogenic Epithelial Cells in Combination with Dental Mesenchymal Cells Produce Enamel–Dentin-Like Complex Structures

Honda, Masaki; Shinohara, Yuko; Hata, Ken‐ichiro

doi:10.3727/000000007783465208

Cited by 41 publications

(33 citation statements)

References 53 publications

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“…DFSCs are able to form cementum and bone tissues in vitro and in vivo (Kemoun et al, 2007;Yokoi et al, 2006). Human dental epithelial stem cells (hDESCs) can be isolated from the third molar that develops late after birth (Honda et al, 2007;Honda et al, 2005). Another source of hDESCs is the epithelial root sheath that disintegrates into strands of epithelial cells, also known as epithelial rests of Malassez (ERM).…”

Section: Cells In Vitro and In Vivomentioning

confidence: 99%

Stem cell-based approaches in dentistry

Mitsiadis

Orsini

Jiménez-Rojo

2015

eCM

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Repair of dental pulp and periodontal lesions remains a major clinical challenge. Classical dental treatments require the use of specialised tissue-adapted materials with still questionable efficacy and durability. Stem cell-based therapeutic approaches could offer an attractive alternative in dentistry since they can promise physiologically improved structural and functional outcomes. These therapies necessitate a sufficient number of specific stem cell populations for implantation. Dental mesenchymal stem cells can be easily isolated and are amenable to in vitro expansion while retaining their stemness. In vivo studies realised in small and large animals have evidenced the potential of dental mesenchymal stem cells to promote pulp and periodontal regeneration, but have also underlined new important challenges. The homogeneity of stem cell populations and their quality control, the delivery method, the quality of the regenerated dental tissues and their integration to the host tissue are some of the key challenges. The use of bioactive scaffolds that can elicit effective tissue repair response, through activation and mobilisation of endogenous stem cell populations, constitutes another emerging therapeutic strategy. Finally, the use of stem cells and induced pluripotent cells for the regeneration of entire teeth represents a novel promising alternative to dental implant treatment after tooth loss. In this mini-review, we present the currently applied techniques in restorative dentistry and the various attempts that are made to bridge gaps in knowledge regarding treatment strategies by translating basic stem cell research into the dental practice.

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Section: Cells In Vitro and In Vivomentioning

confidence: 99%

Stem cell-based approaches in dentistry

Mitsiadis

Orsini

Jiménez-Rojo

2015

eCM

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“…It has also been shown that the presence of the BM can affect the differentiation of stem cells into odontoblasts. 53,54 In addition, the dermal papilla and dermal sheath cells induce the differentiation of keratinocytes into hair follicles. 55 Furthermore, the variation of the underlying fibroblast cells alter the phenotype of the respiratory epithelium between the proximal alveolar or distal tracheal phenotypes.…”

Section: Effect Of Heterotypic Interactions On Epithelial Cellsmentioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

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Recent advances in the fields of microfabrication, biomaterials, and tissue engineering have provided new opportunities for developing biomimetic and functional tissues with potential applications in disease modeling, drug discovery, and replacing damaged tissues. An intact epithelium plays an indispensable role in the functionality of several organs such as the trachea, esophagus, and cornea. Furthermore, the integrity of the epithelial barrier and its degree of differentiation would define the level of success in tissue engineering of other organs such as the bladder and the skin. In this review, we focus on the challenges and requirements associated with engineering of epithelial layers in different tissues. Functional epithelial layers can be achieved by methods such as cell sheets, cell homing, and in situ epithelialization. However, for organs composed of several tissues, other important factors such as (1) in vivo epithelial cell migration, (2) multicell-type differentiation within the epithelium, and (3) epithelial cell interactions with the underlying mesenchymal cells should also be considered. Recent successful clinical trials in tissue engineering of the trachea have highlighted the importance of a functional epithelium for long-term success and survival of tissue replacements. Hence, using the trachea as a model tissue in clinical use, we describe the optimal structure of an artificial epithelium as well as challenges of obtaining a fully functional epithelium in macroscale. One of the possible remedies to address such challenges is the use of bottom-up fabrication methods to obtain a functional epithelium. Modular approaches for the generation of functional epithelial layers are reviewed and other emerging applications of microscale epithelial tissue models for studying epithelial/mesenchymal interactions in healthy and diseased (e.g., cancer) tissues are described. These models can elucidate the epithelial/mesenchymal tissue interactions at the microscale and provide the necessary tools for the next generation of multicellular engineered tissues and organ-on-a-chip systems.

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“…In these studies, epithelia were removed and cells dissociated enzymatically. Precursors were then amplified and associated with MSC (originated from the same tooth) in Stem cells for tooth engineering vitro in contact with biomaterials such as collagen sponges or synthetic polymers (Honda et al, 2005(Honda et al, , 2007bYoung et al, 2002). These approaches are promising for tooth formation and/or regeneration.…”

Section: Periodontal Ligament Stem Cells (Pdlsc)mentioning

confidence: 99%

Stem cells for tooth engineering

Bluteau

Luder

Bari³

et al. 2008

eCM

169

152

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Tooth development results from sequential and reciprocal interactions between the oral epithelium and the underlying neural crest-derived mesenchyme. The generation of dental structures and/or entire teeth in the laboratory depends upon the manipulation of stem cells and requires a synergy of all cellular and molecular events that finally lead to the formation of tooth-specific hard tissues, dentin and enamel. Although mesenchymal stem cells from different origins have been extensively studied in their capacity to form dentin in vitro, information is not yet available concerning the use of epithelial stem cells. The odontogenic potential resides in the oral epithelium and thus epithelial stem cells are necessary for both the initiation of tooth formation and enamel matrix production. This review focuses on the different sources of stem cells that have been used for making teeth in vitro and their relative efficiency. Embryonic, post-natal or even adult stem cells were assessed and proved to possess an enormous regenerative potential, but their application in dental practice is still problematic and limited due to various parameters that are not yet under control such as the high risk of rejection, cell behaviour, long tooth eruption period, appropriate crown morphology and suitable colour. Nevertheless, the development of biological approaches for dental reconstruction using stem cells is promising and remains one of the greatest challenges in the dental field for the years to come.

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Subcultured Odontogenic Epithelial Cells in Combination with Dental Mesenchymal Cells Produce Enamel–Dentin-Like Complex Structures

Cited by 41 publications

References 53 publications

Stem cell-based approaches in dentistry

Stem cell-based approaches in dentistry

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Stem cells for tooth engineering

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