Subcutaneous fat transplantation alleviates diet-induced glucose intolerance and inflammation in mice

Hocking, Samantha; Stewart, Rebecca L.; Brandon, Amanda E.; Suryana, Eurwin; Stuart, Ella; Baldwin, Emily M.; Kolumam, Ganesh; Modrušan, Zora; Junutula, Jagath R.; Gunton, Jenny E.; Medynskyj, Michael; Blaber, Sinead P.; Karsten, Elisabeth; Herbert, Ben R.; James, David E.; Cooney, Gregory J.; Swarbrick, Michael M.

doi:10.1007/s00125-015-3583-y

Cited by 67 publications

(93 citation statements)

References 52 publications

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“…This difference is important because even though pgWAT, unlike omental fat, does not have direct access to the portal vein, the accumulation of pgWAT also results in metabolic disturbances in mice. Moreover, studies in mice have shown that transplantation of SAT from lean mice into the visceral cavity has beneficial effects on body weight, glucose metabolism and insulin sensitivity in lean (Tran et al, 2008) and obese (Foster et al, 2013;Hocking et al, 2015) mice. However, the transplantation of pgWAT to subcutaneous sites has resulted in either no (Barrera et al, 2012;Tran et al, 2008) or some metabolic advantages (Satoor et al, 2011;Tran and Kahn, 2010).…”

Section: Metabolic Risk Is Associated With Individual Adipose Tissue mentioning

confidence: 99%

Heterogeneity of adipose tissue in development and metabolic function

Schoettl

Fischer

Ussar

2018

Journal of Experimental Biology

175

139

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Adipose tissue is a central metabolic organ. Unlike other organs, adipose tissue is compartmentalized into individual depots and distributed throughout the body. These different adipose depots show major functional differences and risk associations for developing metabolic syndrome. Recent advances in lineage tracing demonstrate that individual adipose depots are composed of adipocytes that are derived from distinct precursor populations, giving rise to different populations of energy-storing white adipocytes. Moreover, distinct lineages of energy-dissipating brown and beige adipocytes exist in discrete depots or within white adipose tissue depots. In this Review, we discuss developmental and functional heterogeneity, as well as sexual dimorphism, between and within individual adipose tissue depots. We highlight current data relating to the differences between subcutaneous and visceral white adipose tissue in the development of metabolic dysfunction, with special emphasis on adipose tissue expansion and remodeling of the extracellular matrix. Moreover, we provide a detailed overview of adipose tissue development as well as the consensus and controversies relating to adult adipocyte precursor populations.

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Section: Metabolic Risk Is Associated With Individual Adipose Tissue mentioning

confidence: 99%

Heterogeneity of adipose tissue in development and metabolic function

Schoettl

Fischer

Ussar

2018

Journal of Experimental Biology

175

139

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“…Other HFD studies of Osborne‐Mendel rats , Wistar rats , C57BL/6 , cafeteria‐fed Lister hooded rats and C3H Manchester mice reported increased BF and FI following the diet and surgery compared with chow‐fed animals and increased BW only in Stephens et al . Similarly, an HFD led to increased BW and FI in C57BL/6 mice following WAT being removed or transplanted compared with controls. In contrast, male Wistar rats that underwent BAT and WAT lipectomy or transplantation and were fed either a HFD or high‐carbohydrate diet had decreased BW and BF .…”

Section: Resultsmentioning

confidence: 91%

“…Overall, C57BL/6J mice with WAT or BAT removed had inconclusive results (e.g., some subgroups had lipectomized animals with either a lower BW or no significant changes in BW) . Similarly, C57BL/6J ob/ob mice had no changes in BW or had mixed results.…”

Section: Resultsmentioning

confidence: 99%

A Systematic Scoping Review of Surgically Manipulated Adipose Tissue and the Regulation of Energetics and Body Fat in Animals

Murillo

Kaiser

Smith

et al. 2019

Obesity

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Objective Surgical manipulations of adipose tissue by removal, or partial lipectomy, have demonstrated body fat compensation and recovered body weight, suggesting that the body is able to resist changes to body composition. However, the mechanisms underlying these observations are not well understood. The purpose of this scoping review is to provide an update on what is currently known about the regulation of energetics and body fat after surgical manipulations of adipose tissue in small mammals. Methods PubMed and Scopus were searched to identify 64 eligible studies. Outcome measures included body fat, body weight, food intake, and circulating biomarkers. Results Surgeries performed included lipectomy (72%) or transplantation (12%) in mice (35%), rats (35%), and other small mammals. Findings suggested that lipectomy did not have consistent long‐term effects on reducing body weight and fat because regain occurred within 12 to 14 weeks post surgery. Hence, biological feedback mechanisms act to resist long‐term changes of body weight or fat. Furthermore, whether this weight and fat regain occurred because of “passive” and “active” regulation under the “set point” or “settling point” theories cannot fully be discerned because of limitations in study designs and data collected. Conclusions The regulation of energetics and body fat are complex and dynamic processes that require further studies of the interplay of genetic, physiological, and behavioral factors.

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“…Dietary, surgical and genetic manipulations in male rodents have shown that inflammation, visceral fat and hepatic steatosis are strong determinants shifting obesity towards metabolic disease . In particular, hepatic steatosis may lead to insulin resistance independent of weight gain .…”

Section: Introductionmentioning

confidence: 99%

Sexual dimorphism in hepatic lipids is associated with the evolution of metabolic status in mice

et al. 2017

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Ectopic lipid accumulation in the liver is implicated in metabolic disease in an age-and sex-dependent manner. The role of hepatic lipids has been well established within the scope of metabolic insults in mice, but has been insufficiently characterized under standard housing conditions, where age-related metabolic alterations are known to occur. We studied a total of 10 male and 10 female mice longitudinally. At 3, 7 and 11 months of age, non-invasive 1 H-magnetic resonance spectroscopy ( 1 H-MRS) was used to monitor hepatic lipid content (HLC) and fatty acid composition in vivo, and glucose homeostasis was assessed with glucose and insulin challenges.At the end of the study, hepatic lipids were comprehensively characterized by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometric analyses of liver tissue samples.In males, HLC increased from 1.4 ± 0.1% at 3 months to 2.9 ± 0.3% at 7 months (p < 0.01) and 2.7 ± 0.3% at 11 months (p < 0.05), in correlation with fasting insulin levels (p < 0.01, r = 0.51) and parameters from the insulin tolerance test (ITT; p < 0.001, r = -0.69 versus area under the curve; p < 0.01, r = -0.57 versus blood glucose drop at 1 h post-ITT; p < 0.01, r = 0.55 versus blood glucose at 3 h post-ITT). The metabolic performance of females remained the same throughout the study, and HLC was higher than that of males at 3 months (2.7 ± 0.2%, p < 0.01), but comparable at 7 months (2.2 ± 0.2%) and 11 months (2.2 ± 0.1%). Strong sexual dimorphism in bioactive lipid species, including diacylglycerols (higher in males, p < 0.0001), phosphatidylinositols (higher in females, p < 0.001) and omega-3 polyunsaturated fatty acids (higher in females, p < 0.01), was found to be in good correlation with metabolic scores at 11 months. Therefore, in mice housed under standard conditions, sex-specific composition of bioactive lipids is associated with metabolic protection in females, whose metabolic performance was independent of hepatic cytosolic lipid content.

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Subcutaneous fat transplantation alleviates diet-induced glucose intolerance and inflammation in mice

Abstract: Intra-abdominal transplantation of subcutaneous fat reverses HFD-induced glucose intolerance, hepatic triacylglycerol accumulation and systemic inflammation in mice.

Cited by 67 publications

References 52 publications

Heterogeneity of adipose tissue in development and metabolic function

Heterogeneity of adipose tissue in development and metabolic function

A Systematic Scoping Review of Surgically Manipulated Adipose Tissue and the Regulation of Energetics and Body Fat in Animals

Sexual dimorphism in hepatic lipids is associated with the evolution of metabolic status in mice

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