Subcutaneous‐induced membranes have no osteoinductive effect on macroporous HA‐TCP in vivo

Abstract

Induced Membranes Technique was first described to enhance bone reconstruction of large osseous defects. Previous in vitro studies established their osteoinductive potential, due to the presence of opteoblasts precursors and to high amounts of growth factors contained within. The purpose of this study was to test in vivo the osteoinductive properties of induced membranes on a macroporous HA-TCP in a nonosseous subcutaneous site. Subcutaneous-induced membranes were obtained in 21 rabbits; 1 month later, the membranes were filled with a biphasic calcium phosphate material composed of 75% hydroxyapatite (HA) and 25% beta-tricalcium phosphate associated or not with autograft. Histological and immunohistochemical studies were performed on membrane biopsies. Undecalcified and decalcified sections were qualitatively and quantitatively analyzed. (45)Ca uptake was observed and quantified on the sections using microimager analysis. Dense vascularity was found in the induced membranes. New bone formation was detected in the HA-TCP + autograft samples and increased significantly from 3 to 6 months (p < 0.05). No bone was detected in the biomaterial graft alone in the induced membranes at any time. This study showed that induced membranes placed in a nonosseous site have no osteoinductive properties on a macroporous biphasic calcium phosphate biomaterial.

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

“…Upon comparison to PDC, the induced membrane-derived cells had a similar colony-forming and differentiation capacity; however they did express higher levels SDF1α, a protein that is known to be involved in the recruitment of progenitors to the site of injury. It is currently unclear what the origin of these progenitors is, however, the assumption that they may come from the periosteum is supported as induced membranes which were formed subcutaneously have no bone-forming capacity in conjunction with a calcium phosphate biomaterial [100].…”

Ingénierie tissulaire osseuse en chirurgie buccale et maxillo-faciale : applications cliniques

“…Le principal effet de la membrane est celui de mainteneur d'espace alors que les effets sur la prévention de la résorption du greffon et la sécrétion de facteurs ostéo-inducteurs par la membrane sont encore discutés [22,65]. L'état tissulaire local antérieur a également une influence sur le succès de cette technique car les suites opératoires ont été plus favorables avec les ostéo-myélites (100 % de succès) [9] qu'avec les ostéoradionécroses (50 % de succès) [19].…”

“…Deux articles [9,22] ont présenté des cas de reconstruction mandibulaire par la technique des membranes induites, initialement décrite pour la reconstruction des os longs [23,24]. Cette méthode a été utilisée à la mandibule pour traiter des cas d'ostéomyélites et d'ostéoradioné-croses [9,19].…”

Mandibular reconstruction using induced membranes with autologous cancellous bone graft and HA-βTCP: animal model study and preliminary results in patients

“…The technique is efficient in debilitated patients, but would be more effective in non-irradiated patients in good general health. The authors recently published part of this study showing that subcutaneous induced membranes have no osteoinductive effect on macroporous HA-TCP in vivo 29 . A further study using artificial bones placed subcutaneously with osseous bone growth factors is underway.…”