Subcutaneous Infection Caused by Cladosporium sphaerospermum: A Case Report

Kong, Qingtao; Duan, Yuanyuan; Yuan, F.; Chen, J; Liu, F; Dang, Yongchao; Shi, Haiping

doi:10.1007/s11046-020-00503-3

Cited by 3 publications

(3 citation statements)

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“…The high accuracy rate (100%) using the FA-sandwich method was observed in our tested 9 rare filamentous fungi. The presence of these rare fungi poses a risk to immunocompromised individuals [ 27 ]. Therefore, their accurate and rapid identification could improve the diagnostic capabilities for mycoses in our laboratory, and broadly used in clinical laboratories with limited resources [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Formic acid sandwich method is well-suited for filamentous fungi identification and improves turn around time using Zybio EXS2600 mass spectrometry

Wu,

Ao,

Zheng

et al. 2024

BMC Microbiol

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Objectives Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is extensively employed for the identification of filamentous fungi on MALDI Biotyper (Bruker Daltonics) and Vitek MS (biomerieux), but the performance of fungi identification on new EXS2600 (Zybio) is still unknow. Our study aims to evaluate the new EXS2600 system's (Zybio) ability to rapidly identify filamentous fungi and determine its effect on turnaround time (TAT) in our laboratory. Methods We tested 117 filamentous fungi using two pretreatment methods: the formic acid sandwich (FA-sandwich) and a commercial mold extraction kit (MEK, Zybio). All isolates were confirmed via sequence analysis. Laboratory data were extracted from our laboratory information system over two 9-month periods: pre-EXS (April to December 2022) and post-EXS (April to December 2023), respectively. Results The total correct identification (at the species, genus, or complex/group level) rate of fungi was high, FA-sandwich (95.73%, 112/117), followed by MEK (94.02%, 110/117). Excluding 6 isolates not in the database, species-level identification accuracy was 92.79% (103/111) for FA-sandwich and 91.89% (102/111) for MEK; genus-level accuracy was 97.29% (108/111) and 96.39% (107/111), respectively. Both methods attained a 100% correct identification rate for Aspergillus, Lichtheimia, Rhizopus Mucor and Talaromyces species, and were able to differentiate between Fusarium verticillioides and Fusarium proliferatum within the Fusarium fujikuroi species complex. Notably, high confidence was observed in the species-level identification of uncommon fungi such as Trichothecium roseum and Geotrichum candidum. The TAT for all positive cultures decreased from pre EXS2600 to post (108.379 VS 102.438, P < 0.05), and the TAT for tissue decreased most (451.538 VS 222.304, P < 0.001). Conclusions The FA-sandwich method is more efficient and accurate for identifying filamentous fungi with EXS2600 than the MEK. Our study firstly evaluated the performance of fungi identification on EXS2600 and showed it is suitable for clinical microbiology laboratories use.

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Section: Discussionmentioning

confidence: 99%

Formic acid sandwich method is well-suited for filamentous fungi identification and improves turn around time using Zybio EXS2600 mass spectrometry

Wu,

Ao,

Zheng

et al. 2024

BMC Microbiol

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“…This can provide long-lasting antimicrobial activity against wound infections or other microbial contamination [ 9 , 18 , 21 , 22 , 23 ]. Previous research has indicated the presence of Cladosporium in wound infections, highlighting its potential pathogenicity and its role as an opportunistic pathogen [ 24 , 25 ]. Thus, in this study, ESM samples were extracted using a number of different techniques and further enhanced by incorporating either standard AgNPs that were available commercially (Sigma-Aldrich), or using novel AgNPs manufactured by means of a unique green patented process (Metalchemy, Patent No: GB2598715A).…”

Section: Introductionmentioning

confidence: 99%

A Sustainable, Green-Processed, Ag-Nanoparticle-Incorporated Eggshell-Derived Biomaterial for Wound-Healing Applications

Mensah,

Trotta,

Briggs

et al. 2023

JFB

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The eggshell membrane (ESM) is a natural biomaterial with unique physical and mechanical properties that make it a promising candidate for wound-healing applications. However, the ESM’s inherent properties can be enhanced through incorporation of silver nanoparticles (AgNPs), which have been shown to have antimicrobial properties. In this study, commercially produced AgNPs and green-processed AgNPs were incorporated into ESM and evaluated for their physical, biological, and antimicrobial properties for potential dermal application. The ESM was extracted using various techniques, and then treated with either commercially produced AgNPs (Sigma-Aldrich, Poole, UK) or green-synthesized AgNPs (Metalchemy, London, UK) to produce AgNPs-ESM samples. The physical characteristics of the samples were evaluated using scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, and the biological properties were assessed through in vitro studies using human dermal fibroblasts (HDFs) and BJ cells. The SEM analysis of the AgNPs-ESM samples showed localization of AgNPs on the ESM surface, and that the ESM maintained its structural integrity following AgNP incorporation. The FTIR confirmed loading of AgNPs to ESM samples. The biological studies showed that the 5 μg/mL AgNPs-ESM samples were highly biocompatible with both HDFs and BJ cells, and had good viability and proliferation rates. Additionally, the AgNPs-ESM samples demonstrated pro-angiogenic properties in the CAM assay, indicating their potential for promoting new blood vessel growth. Assessment of the antimicrobial activity of the enhanced AgNPs/ESMs was validated using the International Standard ISO 16869:2008 methodology and exploited Cladosporium, which is one of the most commonly identified fungi in wounds, as the test microorganism (≥5 × 106 cells/mL). The AgNPs-ESM samples displayed promising antimicrobial efficacy as evidenced by the measured zone of inhibition. Notably, the green-synthesized AgNPs demonstrated greater zones of inhibition (~17 times larger) compared to commercially available AgNPs (Sigma-Aldrich). Although both types of AgNP exhibited long-term stability, the Metalchemy-modified samples demonstrated a slightly stronger inhibitory effect. Overall, the AgNPs-ESM samples developed in this study exhibited desirable physical, biological, and antimicrobial properties for potential dermal wound-dressing applications. The use of green-processed AgNPs in the fabrication of the AgNPs-ESM samples highlights the potential for sustainable and environmentally friendly wound-healing therapies. Further research is required to assess the long-term biocompatibility and effectiveness of these biomaterials in vivo.

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“…Among the species most frequently associated with infections in humans are C. oxysporum (cutaneous phaeohyphomycosis with morphological identification), C. sphaerospermum (subcutaneous infection with ITS1-ITS4 molecular identification), C. herbarum (chronic rhinosinusitis with 18S rRNA molecular identification), C. macrocarpum (brain abscess with morphological identification), C. tenuissimum (chromoblastomycosis with ITS and D1/D2 and a D1/D2 domain of 26S rRNA molecular identification), and C. langeronii (chromoblastomycosis with ITS1-ITS4 molecular identification) [10][11][12][13][14]. Reports of C. cladosporioides infection in humans include cases of phaeohyphomycosis identified by morphological identification, keratomycosis with ITS1-18S rRNA molecular identification, pneumonia with morphological identification, rhinosinusitis with morphological identification, and, rarely, chromoblastomycosis with a D1/D2 domain of 26S rRNA molecular identification [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

From Child to Old Man: A Slowly Evolving Case of Chromoblastomycosis Caused by Cladosporium cladosporioides

Rodríguez-Cerdeira,

Hernández-Castro,

Arenas

et al. 2023

Antibiotics

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Chromoblastomycosis is a chronic granulomatous mycosis of the skin and subcutaneous tissue caused by traumatic inoculation with dematiaceous fungi. This disease primarily affects agricultural workers, who are mostly men. We present a case of chromoblastomycosis in a 63-year-old male farmer patient with dermatosis over 50 years of evolution, with warty, erythematous, and scaly plaques that predominate on the left hemithorax. Direct examination with potassium hydroxide (KOH) revealed numerous fumagoid cells. Amplification and sequencing of the internal transcribed spacer (ITS) and translation elongation factor 1-alpha (TEF-1a) gene revealed that chromoblastomycosis was caused by Cladosporium cladosporioides. The chromoblastomycosis was treated with itraconazole and fluconazole without any improvement, and amphotericin B was administered with partial improvement.

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Subcutaneous Infection Caused by Cladosporium sphaerospermum: A Case Report

Cited by 3 publications

References 0 publications

Formic acid sandwich method is well-suited for filamentous fungi identification and improves turn around time using Zybio EXS2600 mass spectrometry

Formic acid sandwich method is well-suited for filamentous fungi identification and improves turn around time using Zybio EXS2600 mass spectrometry

A Sustainable, Green-Processed, Ag-Nanoparticle-Incorporated Eggshell-Derived Biomaterial for Wound-Healing Applications

From Child to Old Man: A Slowly Evolving Case of Chromoblastomycosis Caused by Cladosporium cladosporioides

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