Suberoylanilide hydroxamic acid (SAHA) inhibits transforming growth factor-beta 2-induced increases in aqueous humor outflow resistance

Abstract

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Vorinostat (SAHA) and Breast Cancer: An Overview

“…In addition to chromatin remodeling, HDACs affect non-histone proteins at specific sites, including tumor suppressors [31][32][33] and oncoproteins [34,35] involved in tumor signaling pathways, ultimately affecting cell fate [36]. HDACs deacetylate many groups of non-histone proteins including: transcription factors [37][38][39], cell adhesion proteins [40,41], cellular proteins [42], DNA repair proteins [43], cell signaling proteins [44,45], and viral proteins [46] (Figure 1C). However, HDACs role in tumorigenesis is controversial: either promoting cancer cell survival or causing cell death among different types of cancers [47][48][49][50].…”

A ROCK inhibitor suppresses the transforming growth factor-beta-2-induced endothelial–mesenchymal transition in Schlemm’s canal endothelial cells

“…Cell proliferation was evaluated using the WST-8 assay (Cell Counting Kit-8, CCK-8; CK04, Dojindo, Kumamoto, Japan), as described previously 17 . SC cells were seeded in 96-well plates at 1 × 10 4 cells per well and incubated for 24 h. After serum starvation for 24 h, TGF-β2, Y-27632, BMP4 and SB203580 were added to the cells and incubated for 72 h. CCK-8, which is a detection reagent, was added and the absorbance at 450 nm was measured using a microplate reader (Multiskan FC, Thermo Fisher Scientific) after incubation for 2 h. Cell proliferation is presented as relative change compared to the control.…”

“…Fluorescent immunostaining of SC cells was performed as reported previously 17 , 47 . Phase-contrast images were acquired with an inverted microscope (IX71, Olympus, Tokyo, Japan) before fixation.…”

“…Western blotting was performed as described previously 17 , 30 . Cell lysates were collected from SC cells 24 and 72 h after treatment using LIPA buffer (89900, Thermo Fisher Scientific) with protease inhibitor (78410, Thermo Fisher Scientific) and phosphatase inhibitor (07574-61, Nacarai Tesque, Kyoto, Japan).…”

“…TGF-β2 increases aqueous humor outflow resistance, which is thought to be related to epithelial–mesenchymal transition (EMT) induction of TM cells by TGF-β2, such as increased expression of ECM (fibronectin, collagen) and alpha smooth muscle actin (α-SMA) 12 – 16 . Moreover, TGF-β2 induces the endothelial mesenchymal transition (EndMT) in SC cells 17 , 18 . In addition, SC cells from glaucoma patients have higher expression of α-SMA, fibronectin, and collagen, and higher proliferative potential than those from non-glaucoma eyes 19 , 20 .…”