Suberythemal ultraviolet B radiation alters the expression of cell cycle-related proteins in the epidermis of human subjects without leading to photoprotection

Norval, Mary; Słowik-Rylska, Małgorzata; Jochymski, Cezary; Kozłowski, Wojciech; Sysa-Jędrzejowska, Anna; Rogowski-Tylman, Michał; Lesiak, Aleksandra

doi:10.1111/j.1365-2133.2009.09380.x

Cited by 5 publications

(6 citation statements)

References 31 publications

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“…To test the effects of ultraviolet radiation, HaCaT cells were exposed to a physiologically relevant dose of SSL (4 J/cm 2 UVA and 206 mJ/cm 2 UVB). This corresponds to 1–2 minimum erythemal doses in fair skinned individuals, a dose readily achievable through normal environmental exposure, although the effects of SSL exposure on cultured cells are likely intensified when compared to whole skin as the model does not account for the light filtering ability of an intact stratum corneum [48]. Not surprisingly, folate depletion sensitizes keratinocytes toward apoptosis when they are exposed to SSL.…”

Section: Discussionmentioning

confidence: 99%

Photobiological implications of folate depletion and repletion in cultured human keratinocytes

Williams

Jacobson

2010

Journal of Photochemistry and Photobiology B: Biology

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Folate nutrition is critical in humans and a high dietary folate intake is associated with a diminished risk of many types of cancer. Both synthetic folic acid and the most biologically abundant extracellular reduced folate, 5-methyltetrahydrofolate, are degraded under conditions of ultraviolet radiation (UVR) exposure. Skin is a proliferative tissue with increased folate nutrient demands due to a dependence upon continuous epidermal cell proliferation and differentiation to maintain homeostasis. Regions of skin are also chronically exposed to UVR, which penetrates to the actively dividing basal layer of the epidermis, increasing the folate nutrient demands in order to replace folate species degraded by UVR exposure and to supply the folate cofactors required for repair of photodamaged DNA. Localized folate deficiencies of skin are a likely consequence of UVR exposure. We report here a cultured keratinocyte model of folate deficiency that has been applied to examine possible effects of folate nutritional deficiencies in skin. Utilizing this model, we were able to quantify the concentrations of key intracellular folate species during folate depletion and repletion. We investigated the hypotheses that the genomic instability observed under conditions of folate deficiency in other cell types extends to skin, adversely effecting cellular capacity to handle UVR insult and that optimizing folate levels in skin is beneficial in preventing or repairing the procarcinogenic effects of UVR exposure. Folate restriction leads to rapid depletion of intracellular reduced folates resulting in S-phase growth arrest, increased levels of inherent DNA damage, and increased uracil misincorporation into DNA, without a significant losses in overall cellular viability. Folate depleted keratinocytes were sensitized toward UVR induced apoptosis and displayed a diminished capacity to remove DNA breaks resulting from both photo and oxidative DNA damage. Thus, folate deficiency creates a permissive environment for genomic instability, an early event in the process of skin carcinogenesis. The effects of folate restriction, even in severely depleted, growtharrested keratinocytes, were reversible by repletion with folic acid. Overall, these results indicate that skin health can be positively influenced by optimal folate nutriture.

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Section: Discussionmentioning

confidence: 99%

Photobiological implications of folate depletion and repletion in cultured human keratinocytes

Williams

Jacobson

2010

Journal of Photochemistry and Photobiology B: Biology

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Section: Discussionmentioning

confidence: 99%

“…On the other hand, because of the side effects resulting from UVR exposure, we cannot recommend this therapy for the management of vitamin D status. It is widely accepted that UVB induces DNA damage with subsequent generation of photoproducts, mutation formation and increased synthesis of cyclooxygenase 2; all these phenomena, which may occur even under suberythemal doses of UVB, initiate photocancerogenesis (19)(20)(21). A dilemma arises whether sunbathing can be recommended for psoriasis patients in summer instead of the nUVB therapy as the solar intensity is sufficient to provide cumulative doses comparable (or higher) to those obtained during nUVB treatments (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Vitamin D serum level changes in psoriatic patients treated with narrowband ultraviolet B phototherapy are related to the season of the irradiation

Lesiak

Pawlaczyk

Sysa-Jędrzejowska

et al. 2011

Photoderm Photoimm Photomed

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“…By contrast, low-dose UV phototherapy has been demonstrated to be useful for the treatment of a variety of skin disorders, including psoriasis and atopic dermatitis (9,10,65). An erythemal dose (≥1 × MED) may impair DNA repair mechanisms and lead to cell elimination via apoptosis (66,67). A study of hairless mice irradiated with a single dose of ∼1 × MED UVB (75 mJ/cm 2 ) showed significant disruption of the barrier (38).…”

Section: Beneficial Effects Induced By Uvb In the Epidermal Barriermentioning

confidence: 99%

“…A study of human subjects irradiated with 0.7 × MED UVB for 10 consecutive days revealed an unaltered expression of p53, a protein which is able to activate DNA repair proteins when DNA has sustained damage. This indicated that the repair systems were activated (67). …”

Section: Beneficial Effects Induced By Uvb In the Epidermal Barriermentioning

confidence: 99%

Epidermal barrier: Adverse and beneficial changes induced by ultraviolet B irradiation depending on the exposure dose and time (Review)

Permatasari

Zhou

Luo

2013

Experimental and Therapeutic Medicine

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Exposure of the skin to ultraviolet (UV) radiation induces various harmful effects in the tissues, particularly disruption of the epidermal barrier. However, ultraviolet B (UVB) irradiation has been applied in the treatment of atopic dermatitis, a skin disease in which the epidermal barrier is defective. We reviewed the homeostasis of the epidermal barrier and several studies investigating the adverse and beneficial effects caused by different doses of UVB irradiation in the epidermal barrier. It may be concluded that, despite the harmful effects of UVB irradiation on the skin, UVB irradiation is able to exert beneficial effects in the epidermal barrier when administered in suberythemal doses and over a relatively short period of time, with no clinically evident inflammation or barrier disruption. This may be a useful therapeutic strategy for the use of UVB irradiation in the treatment of skin diseases with a disrupted epidermal barrier, such as atopic dermatitis, while reducing or avoiding the side-effects.

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Suberythemal ultraviolet B radiation alters the expression of cell cycle-related proteins in the epidermis of human subjects without leading to photoprotection

Cited by 5 publications

References 31 publications

Photobiological implications of folate depletion and repletion in cultured human keratinocytes

Photobiological implications of folate depletion and repletion in cultured human keratinocytes

Vitamin D serum level changes in psoriatic patients treated with narrowband ultraviolet B phototherapy are related to the season of the irradiation

Epidermal barrier: Adverse and beneficial changes induced by ultraviolet B irradiation depending on the exposure dose and time (Review)

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