Subgenome chromosome walking in wheat: A 450-kb physical contig in
 Triticum monococcum
 L. spans the
 Lr10
 resistance locus in hexaploid wheat (
 Triticum aestivum
 L.)

Stein, Nils; Feuillet, Catherine; Wicker, Thomas; Schlagenhauf, Edith; Keller, Beat

doi:10.1073/pnas.230361597

Cited by 151 publications

(126 citation statements)

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“…Based on the evolution of the Acc-1 gene, T. urartu (donor of the A genome of T. durum and T. aestivum ) and T. monococcum (donor of the A m genome) were estimated to have diverged only 0.5 to 1 million years ago (Huang et al, 2002a). The genome of T. monococcum has been used successfully as a model for the A genome of hexaploid wheat, confirming the close relationship between the A u and the A m genomes (Stein et al, 2000).…”

Section: Introductionmentioning

confidence: 78%

“…Isolation of genomic DNA, DNA gel blot hybridization, labeling experiments, and linkage analysis were performed as described by Stein et al (2000). Twenty micrograms of genomic DNA was used for hexaploid wheat lines, 13 g was used for tetraploid lines, and 7 g was used for diploid lines.…”

Section: Restriction Fragment Length Polymorphism Analysismentioning

confidence: 99%

“…Screening of the T. monococcum (Lijavetzky et al, 1999) and the T. durum BAC library (http://agronomy.ucdavis.edu/Dubcovsky/BAC-library/ BAC_Langdon.htm) was performed by hybridization as described by Stein et al (2000). BAC DNA preparation for fingerprint analysis and BAC end sequencing were performed as described previously (Stein et al, 2000).…”

Section: Bac Library Screening and Bac Analysismentioning

confidence: 99%

“…BAC DNA preparation for fingerprint analysis and BAC end sequencing were performed as described previously (Stein et al, 2000). BAC clones were assembled into contigs based on their HindIII, NotI, and SalI restriction patterns and hybridization with the BAC ends.…”

Section: Bac Library Screening and Bac Analysismentioning

confidence: 99%

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Rapid Genome Divergence at Orthologous Low Molecular Weight Glutenin Loci of the A and Am Genomes of Wheat[W]

et al. 2003

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To study genome evolution in wheat, we have sequenced and compared two large physical contigs of 285 and 142 kb covering orthologous low molecular weight (LMW) glutenin loci on chromosome 1AS of a diploid wheat species ( Triticum monococcum subsp monococcum ) and a tetraploid wheat species ( Triticum turgidum subsp durum ). Sequence conservation between the two species was restricted to small regions containing the orthologous LMW glutenin genes, whereas Ͼ 90% of the compared sequences were not conserved. Dramatic sequence rearrangements occurred in the regions rich in repetitive elements. Dating of long terminal repeat retrotransposon insertions revealed different insertion events occurring during the last 5.5 million years in both species. These insertions are partially responsible for the lack of homology between the intergenic regions. In addition, the gene space was conserved only partially, because different predicted genes were identified on both contigs. Duplications and deletions of large fragments that might be attributable to illegitimate recombination also have contributed to the differentiation of this region in both species. The striking differences in the intergenic landscape between the A and A m genomes that diverged 1 to 3 million years ago provide evidence for a dynamic and rapid genome evolution in wheat species.

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Section: Introductionmentioning

confidence: 78%

Section: Restriction Fragment Length Polymorphism Analysismentioning

confidence: 99%

Section: Bac Library Screening and Bac Analysismentioning

confidence: 99%

Section: Bac Library Screening and Bac Analysismentioning

confidence: 99%

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Rapid Genome Divergence at Orthologous Low Molecular Weight Glutenin Loci of the A and Am Genomes of Wheat[W]

et al. 2003

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“…Repeated rounds of bacterial artificial chromosome (BAC) library screening, or chromosome walking, are usually necessary to cover the region of interest with a contiguous sequence 8 . Chromosome walking is particularly tedious in crop species that have large and repeat-rich genomes, such as wheat.…”

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confidence: 99%

Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly

et al. 2017

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Cereal crops such as wheat and maize have large repeat-rich genomes that make cloning of individual genes challenging. Moreover, gene order and gene sequences often differ substantially between cultivars of the same crop species. A major bottleneck for gene cloning in cereals is the generation of high-quality sequence information from a cultivar of interest. In order to accelerate gene cloning from any cropping line, we report 'targeted chromosome-based cloning via long-range assembly' (TACCA). TACCA combines lossless genome-complexity reduction via chromosome flow sorting with Chicago longrange linkage to assemble complex genomes. We applied TACCA to produce a high-quality (N50 of 9.76 Mb) de novo chromosome assembly of the wheat line CH Campala Lr22a in only 4 months. Using this assembly we cloned the broad-spectrum Lr22a leaf-rust resistance gene, using molecular marker information and ethyl methanesulfonate (EMS) mutants, and found that Lr22a encodes an intracellular immune receptor homologous to the Arabidopsis thaliana RPM1 protein.DOI: https://doi.org/10.1038/nbt.3877Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-144182 Journal Article Accepted Version Originally published at: Thind, Anupriya Kaur; Wicker, Thomas; Šimková, Hana; Fossati, Dario; Moullet, Odile; Brabant, Cécile; Vrána, Jan; Doležel, Jaroslav; Krattinger, Simon G (2017). Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly. Nature Biotechnology, 35(8) Using this assembly we cloned the broad-spectrum Lr22a leaf-rust resistance gene using molecular marker information and EMS mutants and found that Lr22a encodes an intracellular immune receptor homologous to the Arabidopsis RPM1 protein.While the world population continues to grow, the arable land per capita is decreasing 6 . To ensure food security, agriculture will require high-yielding crops that can withstand diseases, pests and adverse climatic conditions. A better understanding of genes that control these important traits may enable breeding of crop cultivars capable of feeding the 9-10 billion people expected by 2050. triticina isolates [13][14][15][16] . The Lr22a-mediated resistance is not present in young seedlings (<20 days) but is only visible in wheat plants from ~25 days of age.First, in order to evaluate the effectiveness of Lr22a against Swiss P. triticina isolates we inoculated the Lr22a-containing backcross line RL6044 ('Thatcher Lr22a') and the spring wheat cultivar 'Thatcher' with ten P. triticina isolates that were collected in Switzerland. The first leaves of RL6044 developed leaf rust pustules of similar size as the susceptible control 'Thatcher', while we observed complete to moderate resistance on the third leaves of 30-dayold RL6044 plants in comparison to 'Thatcher' (Fig. 1, Supplementary Fig. 1).Lr22a was previously mapped to the short arm of wheat chromosome 2D using microsatellite analysis of the Lr22a-containing wheat line 98B34-T4B 13 . In order to...

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Genome Organization and Comparative Genomics

Devos

Doležel

Feuillet

2009

Wheat Science and Trade

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Subgenome chromosome walking in wheat: A 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat ( Triticum aestivum L.)

Cited by 151 publications

References 34 publications

Rapid Genome Divergence at Orthologous Low Molecular Weight Glutenin Loci of the A and Am Genomes of Wheat[W]

Rapid Genome Divergence at Orthologous Low Molecular Weight Glutenin Loci of the A and Am Genomes of Wheat[W]

Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly

Genome Organization and Comparative Genomics

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