Subgenotype diversity of hepatitis B virus American genotype F in Amerindians from Venezuela and the general population of Colombia

Abstract

The objective of this study was the evaluation of the genetic diversity found in HBV circulating among Venezuelan Amerindians and the general population in Colombia. Phylogenetic analysis of the S region in 194 isolates showed that genotype F is highly predominant in Colombia and Venezuela. This might be related to the genetic background of the population. F3 is the main subgenotype which circulates in both countries. Phylogenetic analysis of 61 complete genome sequences of HBV American genotypes confirms the presence of two genotypes F and H, and 4 F subgenotypes. In Venezuela, subgenotypes F1, F2, and F3 circulate in East and West Amerindians, while only F3 was found among South Amerindians. Japreira community derived from Yucpa Amerindians around 150 years ago. However, several Japreira HBV sequences were forming a clade that can be classified as subgenotype 2b, differing from Yucpa sequences that belong mainly to subgenotype F3. The apparent absence of correlation between the phylogenetic groupings of HBV isolates with the ethnical origin in aboriginal populations might be suggesting a recent origin of HBV American subgenotypes, or a genetic drift effect. J. Med. Virol. 80:20–26, 2008. © 2007 Wiley‐Liss, Inc.

Epidemiology and Molecular Characterization of Hepatitis B Virus Infection in Isolated Villages in the Western Brazilian Amazon

“…45,46 Genotype F has frequently been described in the Amazon, 17,26,[47][48][49] particularly among native populations. 4 Genotype D was found in an isolate from an individual who was not native to the community studied and is similar to genotypes found in the Mediterranean and East Africa.…”

Molecular Evolution of Hepatitis Viruses

“…Seven subgenotypes are described at the moment for genotype A, 9 for genotype B (Thedja et al, 2011), 12 for genotype C (Mulyanto et al, 2011), 7 for gebnotype D (Meldal et al, 2009) and 4 for genotype F (Devesa et al, 2008). No subgenotypes have been found at present inside genotypes E, G and H. This fact migh be due to the fact that these genotypes might be more recent than the other ones.…”

Detección de hepatitis B oculta en donantes de bancos sangre, Colombia 2008-2009

“…Asimismo, se sabe que en la hepatitis B oculta la carga viral es muy baja, razón por la cual la sensibilidad de las pruebas de detección es crítica (26 Otra posible causa para explicar la posibilidad de falsos negativos en las pruebas de amplificación de ácidos nucleicos, se debe a que para el virus de la hepatitis B se han descrito ocho genotipos (A-H); en Colombia y Venezuela, hay una alta prevalencia del virus de la hepatitis B de genotipo F, mientras que en Brasil es menos frecuente (30). Estos genotipos presentan una divergencia mínima de 8 % entre las secuencias del genoma completo; los genotipos F y H son los más divergentes y son autóctonos de América (30,31).…”

“…Estos genotipos presentan una divergencia mínima de 8 % entre las secuencias del genoma completo; los genotipos F y H son los más divergentes y son autóctonos de América (30,31). El HBsAg contiene un epítopo principal para la inducción de respuesta inmunitaria humoral, que se localiza en una región conocida como "determinante a", entre los aminoácidos 99 y 169, y están implicados en la unión de anticuerpos contra el HBsAg.…”