Subgingival lipid A profile and endotoxin activity in periodontal health and disease

Strachan, A. S.; Harrington, Zoë; Mcilwaine, Clare; Jerreat, Matthew; Belfield, Louise; Kilár, Anikó; Jackson, Simon K.; Foey, Andrew; Zarić, Svetislav

doi:10.1007/s00784-018-2771-9

Cited by 11 publications

(10 citation statements)

References 31 publications

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“…To demonstrate clinical feasibility, a skin swab was collected and subsequently centrifuged to collect cells and debris, which was then diluted and injected into the flow system, and the low-abundant protein was detected and quantified (Ertürk et al, 2018). As another example, in 2019 Strachan et al used mass spectrometry to analyse the lipid A isoforms of bacteria in the subgingival plaque of individuals before and after treatment for periodontitis (Strachan et al, 2019). Certain prominent peaks not present in samples from healthy individuals were visible in samples from diseased patients, corresponding to overly phosphorylated and acetylated lipid A isoforms, and these peaks disappeared after periodontal treatment (Strachan et al, 2019).…”

Section: Biosensors For Indirect Microbiome Analysismentioning

confidence: 99%

“…As another example, in 2019 Strachan et al used mass spectrometry to analyse the lipid A isoforms of bacteria in the subgingival plaque of individuals before and after treatment for periodontitis (Strachan et al, 2019). Certain prominent peaks not present in samples from healthy individuals were visible in samples from diseased patients, corresponding to overly phosphorylated and acetylated lipid A isoforms, and these peaks disappeared after periodontal treatment (Strachan et al, 2019). These examples highlight the ability of biosensors to extract meaningful information from human microbial communities, which can be applied to diagnose disease states and monitor treatment outcome.…”

Section: Biosensors For Indirect Microbiome Analysismentioning

confidence: 99%

“…To design a completely POC biosensor, the entire process, from sample preparation to target detection to readout, needs to be portable and rapid (preferably <30 min). This means that any required sample preparation steps, such as centrifugation (Ertürk et al., 2018), liquefaction and homogenization of raw samples (Zhao et al., 2019), and sample hydrolysis (Strachan et al., 2019), need to be converted to portable platforms, along with the detection method itself. Furthermore, interpretation of results to the physician needs to occur seamlessly via a user interface or other visible format, without requiring complex and expensive equipment (Zhou et al., 2015).…”

Section: Where Are We Now?mentioning

confidence: 99%

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The future of microbiome analysis: Biosensor methods for big data collection and clinical diagnostics

Akarapipad

Yoon

2020

Med Devices & Sens

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Worldwide interest in the human microbiome is rapidly expanding, as the microbiome is a potential key to understanding human health and improving diagnostic and treatment strategies. A wide array of microorganisms, including bacteria, archaea, unicellular/multicellular eukaryotes (i.e. fungi and helminths) and viruses, colonize on the human body. Their activity and interactions with each other impact their host (Rowan-Nash, Korry, Mylonakis, & Belenky, 2019). Although sometimes used interchangeably, the word 'microbiota' refers to the collective community of microorganisms residing in a particular environment (such as a region of the body), whereas the word 'microbiome' refers to the entirety of this community's genetic information (Allaband et al., 2019; Kuczynski et al., 2012). The different regions of the human body contain various combinations of microbes that could provide information not only about infectious diseases at specific sites, but also about the potential health conditions of a person (Mimee et al., 2018). It is remarkable to consider that, according to recently revised estimates, the human body houses approximately as many bacteria (the most abundant member of the microbiota) as human cells, with bacteria comprising 0.3% of total body mass (Sender, Fuchs, & Milo, 2016). According to the

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Section: Biosensors For Indirect Microbiome Analysismentioning

confidence: 99%

Section: Biosensors For Indirect Microbiome Analysismentioning

confidence: 99%

Section: Where Are We Now?mentioning

confidence: 99%

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The future of microbiome analysis: Biosensor methods for big data collection and clinical diagnostics

Akarapipad

Yoon

2020

Med Devices & Sens

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“…Dental plaque or biofilm is composed of microcolonies of bacterial cells, nonrandomly distributed in a shaped matrix or glycocalyx, which subsequently mineralizes to form calculus (Kumar, Swarga Jyoti, Sonowal, & Chawla, 2015). Periodontopathogenic bacteria secrete lipopolysaccharide (LPS), an endotoxin which is absorbed by the most superficial layer of root cementum (Aspriello, Piemontese, Levrini, & Sauro, 2011;Gibbons, 1989;Strachan et al, 2018). Aleo, De Renzis, and Faber (1975) indicated that human gingival fibroblasts did not adhere in vitro to a root surface contaminated with LPS, and assessed that this was possible only when the cementum was removed by root planing.…”

Section: Introductionmentioning

confidence: 99%

A comparative scanning electron microscopy study between the effect of an ultrasonic scaler, reciprocating handpiece, and combined approach on the root surface topography in subgingival debridement

Dassatti

Manicone

Lauricella

et al. 2020

Clinical & Exp Dental Res

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Objective This study aimed to analyze the effectiveness of root‐shape inserts mounted on a reciprocating handpiece during the procedure of root surface debridement (RSD) on extracted teeth. Three different approaches were compared: ultrasonic scaling, employment of root‐shape inserts mounted on a reciprocating handpiece, and a combination of both. Materials and Methods A total of 51 extracted teeth were divided into three groups. The first group was instrumented with an ultrasonic scaler, the second group with flexible root‐shape inserts mounted on a reciprocating handpiece (grain size 40, 15, and 4 μm), whereas the final group underwent a combination of both approaches. The time required for the instrumentation was taken. The specimens were subjected to optical and scanning electron microscopy (SEM), and the photographs were evaluated by three examiners who were blinded to the study. The parameters included were: SEM roughness index (SRI) for the roughness calculation, remaining calculus Index (RCI) to evaluate the residual calculus deposits, and loss of tooth substance index (LTSI) to evaluate the loss of tooth substance caused by instrumentation. Result The results revealed that the time taken for the instrumentation was on average longer when the root‐shape inserts were employed alone, meanwhile the combined approach did not show significant difference in comparison with the ultrasonic scaling. The lower average RCI was obtained with a combined approach. The use of root‐shape inserts seems to cause a moderate increase in LTSI, especially in a combined approach, whereas it resulted in a better average SRI. Conclusion The employment of root‐shape inserts seems to be effective in the RSD for its ability to obtain a smooth and calculus‐free instrumented surface, especially when used in combination with an ultrasonic scaler, and their use can so represent a valid approach to be tested in further in vivo studies.

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“…Nas bactérias Gram positivas, destaca-se o ácido lipoteicóido (lipoteichoic acid -LTA) e nas Gram negativas, uma endotoxina denominada lipopolissacarídeo (lipopolysaccharide -LPS) (Siqueira et al, 2002;Staquet et al, 2011). O LPS é um complexo glicolipídico composto por um polissacarídeo hidrofílico e um domínio hidrofóbico, conhecido como lípide A, responsável pelos efeitos biológicos desta endotoxina (Strachan et al, 2019). As bactérias Gram negativas são comumente encontradas em polpas necróticas, sendo os principais microrganismos envolvidos em infecções endodônticas (Fabricius et al, 1982;Morrison;Ryan, 1987).…”

Section: Scaps E Lipopolissacarídeo (Lps)unclassified

Papel de Endocanabinoides na modulação de células-tronco de Papila Apical in vitro

Meneses¹

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À minha colega e amiga Laís Nicolay Pizzato, que considero um grande e precioso presente que o Doutorado me deu! Fomos parceiras de pesquisa, de laboratório e de sala de aula, mas também de boas risadas, choros, desesperos, festas e churrascos! Sua amizade é algo tão precioso para mim que me sinto de certa forma responsável por você! Obrigada pela sua felicidade contagiante, pelas suas gargalhadas e por sua alegria de viver! Não existe ser humano capaz de não gostar de você! Sei que você vibra com minhas conquistas e torce incansavelmente pela minha felicidade! O mesmo peço a Deus que reserve a você! Conte comigo para sempre, loira! Às minhas amigas do Paraná, Anne Caroline Figueiredo Marassi, que sempre torceu por mim e acompanhou cada conquista e dificuldade desta trajetória, obrigada por me escutar, por pedir meus conselhos, por me dar ideias, por me enviar orações e por fazer grandes planos profissionais para nós! Sua amizade é um presente de Deus em minha vida! Luana Bevilaqua, obrigada pela sua preciosa amizade, pelas conversas, por estar sempre pronta para me buscar e levar para o aeroporto, por se fazer presente em minha vida mesmo estando tão longe! Você é uma amiga para todas as horas, todos os momentos, independentemente de data ou local! Marcia Busato, obrigada pela sua amizade e por me enviar energias positivas e mensagens de carinho! Conte sempre comigo quando precisar! Tatiana Assumpção Iachinski, obrigada por torcer pelas minhas conquistas e vitórias. Admiro demais sua força e inteligência! Daniela Tecchio Painelli, Jacqueline Maffessoni, Roberta Maffessoni, Scheila Kazmierski, minhas amigas super poderosas que tudo resolvem, tudo descobrem e tudo programam! Amo nossas reuniões, conversas e risadas! Tenho imensas saudades de vocês! Satio Inagaki, o ninja da Endodontia, meu mestre e, principalmente, meu grande amigo! Sei que posso contar contigo em todos os momentos e espero que saibas que estarei sempre pronta a lhe ajudar, no que precisar! Hermano Paiva, meu amigo e "irmão mais novo", como ele mesmo diz! Você é precioso, um diamante sendo lapidado! Você é exemplo de simplicidade, comprometimento, dedicação e respeito por tudo o que faz! Obrigada pelas vezes que confiou em mim e compartilhou comigo suas dúvidas, ensinamentos, dilemas e Palavras-chave: Células-tronco de papila apical. Endocanabinoides. Expressão gênica. Receptores canabinóides. TRPV-1. ABSTRACT Meneses CCB. The role of Endocannabinoids in the modulation of stem cells of the Apical Papilla in vitro [thesis].

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Subgingival lipid A profile and endotoxin activity in periodontal health and disease

Cited by 11 publications

References 31 publications

The future of microbiome analysis: Biosensor methods for big data collection and clinical diagnostics

The future of microbiome analysis: Biosensor methods for big data collection and clinical diagnostics

A comparative scanning electron microscopy study between the effect of an ultrasonic scaler, reciprocating handpiece, and combined approach on the root surface topography in subgingival debridement

Papel de Endocanabinoides na modulação de células-tronco de Papila Apical in vitro

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