Subgroup F adenovirus growth in foetal intestinal organ cultures

Abstract

An in vitro foetal intestinal organ culture system was employed to determine the permissiveness of human intestinal cells for subgroup F adenovirus infection. Ad40 and Ad41 growth, monitored through group-specific hexon antigen production, was poor in comparison to that of Ad2 in these cultures, further demonstrating their fastidious nature in most human cells. The low growth capability of these viruses in culture, in relation to their association with gastrointestinal disease is discussed.

Infection kinetics of human adenovirus serotype 41 in HEK 293 cells

“…These results reinforce the hypothesis that this virus may have an active mechanism of viral release and that this could be more efficient in certain cell lines. The block on viral release was also observed in experimental infection of foetal intestinal organ cultures, suggesting an adaptation of HAdV-41 to epithelia that turn over rapidly (Tiemessen et al 1993). It is also possible that the release of viral particles may be dependent upon the presence of proteolytic enzymes and low pH, which are present in the gut but not in culture.…”

“…Human adenovirus F species replicate quite well in the intestinal epithelia (Uhnoo et al 1984, Tiemessen et al 1993). However, these viruses have been considered fastidious in culture (Kidd & Madeley 1981, Brown et al 1984).…”

Adenovirus Infection of Human Enteroids Reveals Interferon Sensitivity and Preferential Infection of Goblet Cells

“…Additionally, most of these primary cells were derived from locations not normally infected by enteric viruses, further complicating their utility in under-standing HAdV-F biology. Interestingly, previous attempts to culture HAdV-F in fetal intestinal organ cultures did not result in infectious virus (43). Thus, human enteroids are the only viable system for studying HAdV-F interactions with mature primary intestinal epithelial cells.…”

Estudo da interação do adenovírus humano, sorotipo 41 (HAdV-41), com células permissivas.

“…Os adenovírus entéricos se replicam muito bem no intestino de crianças com diarréia, com liberação de 10 11 partículas virais por grama de fezes (Uhnoo et al, 1984). Contudo, esses vírus são fastidiosos em cultura celular, sugerindo uma forte adaptação da replicação viral no epitélio intestinal (Tiemessen et al,1993).…”

“…Os HAdV-F (HAdV-40 e 41) apresentam características moleculares, biológicas e estruturais diferentes dos demais adenovírus (Tiemessen et al, 1993;Tiemessen e Kidd, 1995).…”