Subinhibitory Concentrations of Antibiotics Alter the Response of Klebsiella pneumoniae to Components of Innate Host Defense

Opoku‐Temeng, Clement; Freedman, Brett A.; Porter, Adeline R.; Kobayashi, Scott D.; Chen, Liang; Kreiswirth, Barry N.; DeLeo, Frank R.

doi:10.1128/spectrum.01517-22

Cited by 2 publications

(3 citation statements)

References 40 publications

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“…This phenomenon has been shown to impact virulence expression and the interaction with the host immune system in different pathogens, including K. pneumoniae [37–39]. Moreover, the horizontal transfer of some GI families is promoted in the presence of antibiotics by the activation of the excision and transfer machinery [40–42].…”

Section: Resultsmentioning

confidence: 99%

“…In the environment and host tissues, bacteria are exposed to subinhibitory concentrations of antibiotics due to agricultural activities, wastewater treatment or incorrect therapy in the clinical setting, among other reasons [36]. This phenomenon has been shown to impact virulence expression and the interaction with the host immune system in different pathogens, including K. pneumoniae [37][38][39]. Moreover, the horizontal transfer of some GI families is promoted in the presence of antibiotics by the activation of the excision and transfer machinery [40][41][42].…”

Section: Subinhibitory Concentrations Of Clinically Relevant Antibiot...mentioning

confidence: 99%

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Subinhibitory antibiotic concentrations promote the excision of a genomic island carried by the globally spread carbapenem-resistant Klebsiella pneumoniae sequence type 258

Piña-Iturbe,

Hoppe-Elsholz,

Suazo

et al. 2023

Microbial Genomics

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The ICEKp258.2 genomic island (GI) has been proposed as an important factor for the emergence and success of the globally spread carbapenem-resistant Klebsiella pneumoniae sequence type (ST) 258. However, a characterization of this horizontally acquired element is lacking. Using bioinformatic and experimental approaches, we found that ICEKp258.2 is not confined to ST258 and ST512, but also carried by ST3795 strains and emergent invasive multidrug-resistant pathogens from ST1519. We also identified several ICEKp258.2-like GIs spread among different K. pneumoniae STs, other Klebsiella species and even other pathogen genera, uncovering horizontal gene transfer events between different STs and bacterial genera. Also, the comparative and phylogenetic analyses of the ICEKp258.2-like GIs revealed that the most closely related ICEKp258.2-like GIs were harboured by ST11 strains. Importantly, we found that subinhibitory concentrations of antibiotics used in treating K. pneumoniae infections can induce the excision of this GI and modulate its gene expression. Our findings provide the basis for the study of ICEKp258.2 and its role in the success of K. pneumoniae ST258. They also highlight the potential role of antibiotics in the spread of ICEKp258.2-like GIs among bacterial pathogens.

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Section: Resultsmentioning

confidence: 99%

Section: Subinhibitory Concentrations Of Clinically Relevant Antibiot...mentioning

confidence: 99%

Subinhibitory antibiotic concentrations promote the excision of a genomic island carried by the globally spread carbapenem-resistant Klebsiella pneumoniae sequence type 258

Piña-Iturbe,

Hoppe-Elsholz,

Suazo

et al. 2023

Microbial Genomics

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show abstract

“…The copyright holder for this preprint this version posted August 11, 2023. ; https://doi.org/10.1101/2023.08.10.552780 doi: bioRxiv preprint phenomenon has been shown to impact virulence expression and the interaction with the host immune system in different pathogens, including K. pneumoniae (26)(27)(28). Moreover, the horizontal transfer of some GI families is promoted in the presence of antibiotics by the activation of the excision and transfer machinery (29)(30)(31).…”

Section: Subinhibitory Concentrations Of Clinically Relevant Antibiot...mentioning

confidence: 99%

Subinhibitory antibiotic concentrations promote the excision of a genomic island carried by the globally spread carbapenem-resistantKlebsiella pneumoniaeST258

Piña-Iturbe,

Hoppe-Elsholz,

Suazo

et al. 2023

Preprint

View full text Add to dashboard Cite

The ICEKp258.2 genomic island (GI) has been proposed as an important factor for the emergence and success of the globally spread carbapenem-resistant Klebsiella pneumoniae sequence type (ST) 258. However, a characterization of this horizontally acquired element is lacking. Using bioinformatic and experimental approaches, we found that ICEKp258.2 is not confined to ST258 and ST512 but also carried by ST3795 strains and emergent invasive multidrug-resistant pathogens from ST1519. We also identified several ICEKp258.2-like GIs spread among different K. pneumoniae STs, other Klebsiella species, and even other pathogen genera, uncovering horizontal gene transfer events between different STs and bacterial genera. Also, in agreement with the origin of ST258 from ST11, the comparative and phylogenetic analyses of the ICEKp258.2-like GIs suggested that ICEKp258.2 was acquired from an ST11 strain. Importantly, we found that subinhibitory concentrations of antibiotics used in treating K. pneumoniae infections can induce the excision of this GI and modulate its gene expression. Our findings provide the basis for the study of ICEKp258.2 and its role in the success of K. pneumoniae ST258. They also highlight the potential role of antibiotics in the spread of ICEKp258.2-like GIs among bacterial pathogens.

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Subinhibitory Concentrations of Antibiotics Alter the Response of Klebsiella pneumoniae to Components of Innate Host Defense

Cited by 2 publications

References 40 publications

Subinhibitory antibiotic concentrations promote the excision of a genomic island carried by the globally spread carbapenem-resistant Klebsiella pneumoniae sequence type 258

Subinhibitory antibiotic concentrations promote the excision of a genomic island carried by the globally spread carbapenem-resistant Klebsiella pneumoniae sequence type 258

Subinhibitory antibiotic concentrations promote the excision of a genomic island carried by the globally spread carbapenem-resistantKlebsiella pneumoniaeST258

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