Sublethal and lethal toxicity of aluminum industry effluents to early developmental stages of the Crassostrea gigas oyster

His, Édouard; Beiras, Ricardo; Seaman, Matthias; Pagano, Giovanni; Trieff, Norman M.

doi:10.1007/bf00212291

Cited by 20 publications

(18 citation statements)

References 23 publications

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“…Changes in constituting properties of haemolymph can occur due to various pollutants and environmental factors (His et al 1996;Xue & Tristan 2000). Among the constituting properties of haemolymph, an increase in GOT and GPT activation is generally caused by inflow to the haemolymph of cells separated due to tissue damage by environmental contaminants (Casillas et al 1982).…”

Section: Discussionmentioning

confidence: 99%

Characterization and mRNA expression of Mn-SOD and physiological responses to stresses in the Pacific oysterCrassostrea gigas

Park

Choi

et al. 2009

Marine Biology Research

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Superoxide dismutases (SODs) are metalloenzymes that play an important role in mollusc immune defence systems by eliminating oxidative stress to reactive oxygen species. We investigated physiological changes in the Pacific oyster Crassostrea gigas caused by exposure to pollutants (cadmium, tributyltin) and acute water temperature change. We analysed mRNA expression of Mn-SOD in gills using a quantitative polymerase chain reaction (QPCR), and measured the glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and hydrogen peroxide (H 2 O 2 ) levels in haemolymph based on time-and dose-related effects of pollutants and acute water temperature treatments. We cloned a cgMn-SOD full-length cDNA that included an open reading frame of 675 nucleotides that was predicted to encode proteins of 225 amino acids. BLAST analysis of other species indicated that residues essential to the enzymatic functions of Mn-SOD proteins are highly conserved. Levels of Mn-SOD mRNA expression, GOT and GPT were gradually increased and then decreased during the experimental periods. On the other hand, H 2 O 2 levels increased continuously during the exposure periods. These results suggest that Mn-SOD plays an important role in the physiological changes related to metabolism and cell protection that occur in C. gigas when exposed to oxidative stress by pollutants and fluctuations in water temperature.

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Section: Discussionmentioning

confidence: 99%

Characterization and mRNA expression of Mn-SOD and physiological responses to stresses in the Pacific oysterCrassostrea gigas

Park

Choi

et al. 2009

Marine Biology Research

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“…Previous investigations have focused on bauxite manufacturing sludge either as plant effluent [2,3] or as marine sediment from a coastal disposal site (Pagano et al, unpublished data). Aluminum bioaccumulation has been reported in marine plants collected nearby a coastal bauxite sludge disposal site [4], and adverse effects on a benthic macroinvertebrate community were also reported in a study of a bauxite sludgepolluted lake [5].…”

Section: Introductionmentioning

confidence: 99%

“…The issue of bauxite manufacturing by-products may be viewed as a subject of environmental concern, as bauxite byproducts may affect either marine or freshwater benthic com-* To whom correspondence may be addressed (gbpagano@tin.it). munities in the vicinity of sludge disposal sites [2][3][4][5]; (G. Pagano et al, unpublished data).…”

Section: Introductionmentioning

confidence: 99%

Bauxite Manufacturing Residues From Gardanne (France) and Portovesme (Italy) Exert Different Patterns of Pollution and Toxicity to Sea Urchin Embryos

Pagano¹,

Biase²,

Iaccarino³

et al. 2002

Environ Toxicol Chem

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This study was designed to investigate the composition and toxicity of solid residues from bauxite manufacturing plants. Soil and dust samples were collected in the proximity of two bauxite plants (Gardanne, France, and Portovesme, Italy). Samples were analyzed for their content of some selected inorganic contaminants by means of inductively coupled plasma optical emission spectroscopy (ICP-OES) either following acid digestion procedures or by seawater release of soluble components. Toxicity was tested by sea urchin bioassays to evaluate a set of toxicity endpoints including acute embryotoxicity, developmental defects, changes in sperm fertilization success, transmissible damage from sperm to the offspring, and cytogenetic abnormalities. Inorganic analysis showed two distinct sets of inorganic contaminants in Gardanne versus Portovesme, including Al, Cr, Cu, Fe, Mn, Pb, Ti, and Zn; sample composition (seawater-soluble contaminants) and toxicity showed a noteworthy association. The most severe toxicity to embryogenesis and to sperm fertilization success was exerted by some Portovesme samples (0.03-0.5% w/v), with a significant association between toxicity and dose-related seawater release of Zn, Pb, and Mn. Seawater extraction of a toxic dust sample (G20) from the Gardanne factory showed increasing seawater release of Al, Fe, and Mn; the G20 sample, at the level of 0.5%, affected both developing sea urchin embryos and sperm (offspring quality). Soil samples around the Gardanne factory showed the highest frequency of toxic soil sites eastward from the factory. The present data point to solid deposition from bauxite plants as a potential subject of environmental health concern. The results suggest that extraction methods for evaluating the toxicity of complex mixtures should be based on the environmental availability of mixture components. The differences in sample toxicity among the tested sites, however, suggest possible site-to-site variability in geochemical and/or technological parameters.

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“…In our study, the likely source is the effluent outlet located at 420 m depth in the area of experimentation. It comprises mainly arsenic, titanium, aluminium, chromium, iron and manganese [30]. Near the outlet, located slightly upstream of station 2, the concentrations reach 1815 µg g −1 (dry weight) of effluent, and the chromium concentrations in the sediments close to stations 1 and 4 are 106 and 601 µg g −1 of dry sediment, respectively (IFREMER, unpublished data).…”

Section: Discussionmentioning

confidence: 97%

Deep-sea caging of the musselMytilus galloprovincialis: Potential application in ecotoxicological studies

Galgani

Chiffoleau

Gall

et al. 2005

Chemistry and Ecology

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We experimented with caging the Mediterranean mussel (Mytilus galloprovincialis) at various depths for 69 d to measure basic physiological parameters, histological response and bio-accumulation of contaminants in a deep-sea contaminated area. In preliminary experiments, we demonstrated, under artificial pressure conditions, the ability of mussels Mytilus galloprovincialis to tolerate rapid immersion (at a speed of up to 120 m min −1 ). In situ experiments were performed using submerged lines enabling mussels to be maintained at depths ranging of 40-1550 m with survival rates ranging from 80 to 38%, respectively. No significant differences in condition indexes were observed between treated and control specimens. However, histological observations demonstrated a clear reduction in thickness of the digestive epithelium with increasing depth exposure. By determining the contaminants in caged mussels, we found the following values for chromium accumulation: 27.4 µg g −1 dry weight at 580 m depth and 9.8 µg g −1 dry weight at 1550 m. Selected stations were located downstream of an industrial effluent at 420 m. The biological and environmental consequences of deep-sea contamination demonstrate the suitability of caged mussels for monitoring contaminant accumulation.

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Sublethal and lethal toxicity of aluminum industry effluents to early developmental stages of the Crassostrea gigas oyster

Cited by 20 publications

References 23 publications

Characterization and mRNA expression of Mn-SOD and physiological responses to stresses in the Pacific oysterCrassostrea gigas

Characterization and mRNA expression of Mn-SOD and physiological responses to stresses in the Pacific oysterCrassostrea gigas

Bauxite Manufacturing Residues From Gardanne (France) and Portovesme (Italy) Exert Different Patterns of Pollution and Toxicity to Sea Urchin Embryos

Deep-sea caging of the musselMytilus galloprovincialis: Potential application in ecotoxicological studies

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