Sublethal concentrations of triclosan elicited oxidative stress, DNA damage, and histological alterations in the liver and brain of adult zebrafish

Gyimah, Eric; Dong, Xiyang; Qiu, Wenhui; Zhang, Zhen; Xu, Hai

doi:10.1007/s11356-020-08232-2

Cited by 54 publications

(28 citation statements)

References 50 publications

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“…Decline in the expression of both these genes after 96h exposure is supported by earlier studies which also reported a declined expression of Phospholipid hydroperoxide glutathione peroxidase (PHGPx) in liver of tadpole Bufo gargarizans (Chai et al 2017), GPX4b in male juvenile zebra sh (Wang et al 2019a), GPX1a in gill and ovary of zebra sh (Wang et al 2019b). Signi cant inhibition of GR expression in the TCS exposed larvae in the present study is corroborated by the nding of Falisse et al (2017) and Gyimah et al (2020) who also reported declined GR activity in zebra sh early life stages and in the liver of adult zebra sh, respectively. LDH is an important glycolytic enzyme responsible for energy production during anaerobic conditions and plays a very important role to meet the energy demand during stress conditions (Tseng et al 2008).…”

Section: Discussionsupporting

confidence: 89%

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Sharma

Dar

Singh

et al. 2021

Preprint

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Triclosan (TCS) used commonly in pharmaceuticals and personal care products has become the most common pollutant in water. Three days old hatchlings of an indegenous fish, Labeo rohita were given 96h exposure to an environmentally relevant (0.06mg/L) and two moderately lethal concentrations (0.067 and 0.097 mg/L) of TCS and kept for 10 days of recovery for recording transcriptomic alterations in antioxidant/detoxification (SOD, GST, CAT, GPx, GR, CYP1a and CYP3a), metabolic (LDH, ALT and AST) and neurological (AchE) genes and DNA damage. The data were subjected to Principal Component Analysis (PCA) for obtaining biomarkers for the toxicity of TCS. Hatchlings were highly sensitive to TCS (96h LC 50 = 0.126mg/L and risk quotient = 40.95), 96h exposure caused significant induction of CYP3a, AChE and ALT but suppression of all other genes. However, expression of all the genes increased significantly (except for a significant decline in ALT) after recovery. Concentration dependent increase was also observed in DNA damage [Tail Length (TL), Tail Moment (TM), Olive Tail Moment (OTM) and Percent Tail DNA (TDNA)] after 96h. The damage declined significantly over 96h values at 0.06 and 0.067 mg/L after recovery, but was still several times more than control. TCS elicited genomic alterations resulted in 5-11% mortality of exposed hatchlings during the recovery period. It is evident that hatchlings of L. rohita are a potential model and PCA shows that OTM, TL, TM, TDNA, SOD and GR (association with PC1 during exposure and recovery) are the biomarkers for the toxicity of TCS.

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Section: Discussionsupporting

confidence: 89%

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Sharma

Dar

Singh

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 89%

“…Higher level of CYPs in the exposed larvae can also be related to the concentration dependent increase in DNA damage as the CYPs are known to catalyze the formation of genotoxic or mutagenic intermediates from a number of polycyclic aromatic hydrocarbons (Shimada et al 1996). The results of the comet assay in our study are supported by Gyimah et al (2020) who also recorded similar effects of TCS on the liver cells of zebra sh. Association of OTM, TL, TM, TDNA, CYP3a, SOD, GR, GPx, ALT and AST with PC1 after 96h exposure indicates that inspite of TCS in icted DNA damage, the antioxidative/detoxi cation/metabolic genes tried to overcome the stress.…”

Section: Discussionsupporting

confidence: 88%

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Sharma

Dar

Singh

et al. 2021

Environ Sci Pollut Res

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Triclosan (TCS) used commonly in pharmaceuticals and personal care products has become the most common pollutant in water. Three days old hatchlings of an indegenous sh, Labeo rohita were given 96h exposure to an environmentally relevant (0.06mg/L) and two moderately lethal concentrations (0.067 and 0.097 mg/L) of TCS and kept for 10 days of recovery for recording transcriptomic alterations in antioxidant/detoxi cation (SOD, GST, CAT, GPx, GR, CYP1a and CYP3a), metabolic (LDH, ALT and AST) and neurological (AchE) genes and DNA damage. The data were subjected to Principal Component Analysis (PCA) for obtaining biomarkers for the toxicity of TCS. Hatchlings were highly sensitive to TCS (96h LC 50 = 0.126mg/L and risk quotient = 40.95), 96h exposure caused signi cant induction of CYP3a, AChE and ALT but suppression of all other genes. However, expression of all the genes increased signi cantly (except for a signi cant decline in ALT) after recovery. Concentration dependent increase was also observed in DNA damage [Tail Length (TL), Tail Moment (TM), Olive Tail Moment (OTM) and Percent Tail DNA (TDNA)] after 96h. The damage declined signi cantly over 96h values at 0.06 and 0.067 mg/L after recovery, but was still several times more than control. TCS elicited genomic alterations resulted in 5-11% mortality of exposed hatchlings during the recovery period. It is evident that hatchlings of L. rohita are a potential model and PCA shows that OTM, TL, TM, TDNA, SOD and GR (association with PC1 during exposure and recovery) are the biomarkers for the toxicity of TCS.

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“…As shown in Figure 4 b, the expression of GPX in the BPC, BPF, and BPS treated groups marginally increased from after 48 h to 72 h of exposure and decreased during other exposure time periods. Pikulkaew et al detected low expression levels of ERβ1 after 8 h post-fertilization, which then increased from 24 h to 48 h post-fertilization in zebrafish [ 29 ] A significant decrease of CAT and GPX in the brain and liver of zebrafish was reported by Gyimah et al, which may cause damage to the organs of zebrafish [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Study on the Joint Toxicity of BPZ, BPS, BPC and BPF to Zebrafish

et al. 2021

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Bisphenol Z (BPZ), bisphenol S (BPS), bisphenol C (BPC), and bisphenol F (BPF) had been widely used as alternatives to bisphenol A (BPA), but the toxicity data of these bisphenol analogues were very limited. In this study, the joint toxicity of BPZ, BPS, BPC, and BPF to zebrafish (Danio rerio) was investigated. The median half lethal concentrations (LC50) of BPZ, BPS, BPC, and BPF to zebrafish for 96 h were 6.9 × 105 µM, 3.9 × 107 µM, 7.1 × 105 µM, and1.6 × 106 µM, respectively. The joint toxicity effect of BPF–BPC (7.7 × 105–3.4 × 105µM) and BPZ–BPC (3.4 × 105–3.5 × 105µM) with the same toxic ratio showed a synergistic effect, which may be attributed to enzyme inhibition or induction theory. While the toxicity effect of the other two bisphenol analogue combined groups and multi-joint pairs showed an antagonistic effect due to the competition site, other causes need to be further explored. Meanwhile, the expression levels of the estrogen receptor genes (ERα, ERβ1) and antioxidant enzyme genes (SOD, CAT, GPX) were analyzed using a quantitative real-time polymerase chain reaction in zebrafish exposure to LC50 of BPZ, BPS, BPC, and BPF collected at 24, 48, 72, and 96 h. Relative expression of CAT, GPX, and ERβ1 mRNA declined significantly compared to the blank control, which might be a major cause of oxidant injury of antioxidant systems and the disruption of the endocrine systems in zebrafish.

show abstract

Sublethal concentrations of triclosan elicited oxidative stress, DNA damage, and histological alterations in the liver and brain of adult zebrafish

Cited by 54 publications

References 50 publications

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Study on the Joint Toxicity of BPZ, BPS, BPC and BPF to Zebrafish

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