Sublethal effects of chlorantraniliprole on biological characteristics, detoxifying enzyme activity and gene expression profile in the Allium mongolicum Regel leaf beetle Galeruca daurica (Coleoptera: Chrysomelidae)

Abstract

Allium mongolicum Regel leaf beetle, Galeruca daurica (Coleoptera: Chrysomelidae) is rampantly harmful in Inner Mongolia grassland. However, the current management strategies for this pest still heavily rely on chemical control using traditional insecticides or those with novel action. In the study, we conducted an indoor bioassay to evaluate the sublethal effects of chlorantraniliprole on the biological characteristics. Additionally, we assessed the activity of detoxification enzymes, specifically the primary enzymes carboxylesterase (CarE) and cytochrome P450, and insect's secondary metabolic enzymes, namely glutathione S‐transferase (GST) and uridine diphosphate glycosyltransferase (UGT), as well as its gene expression profile. The developmental period of the 3rd instar larvae of G. daurica was significantly prolonged after treatment with chlorantraniliprole, which negatively affected the hatching, pupation and diapause rates as well as their body weight. The larvae showed different dynamics of the enzyme activities within 24 h of chlorantraniliprole treatment. Different sublethal concentrations of chlorantraniliprole showed an inducing effect on the activities, while no significant difference in activity was observed for P450. Expression profiling of detoxifying enzyme genes screened by transcriptome data has revealed that 6 CarE, 2 GST, 7 UGT and 31 P450 genes were up‐regulated. In conclusion, chlorantraniliprole's sublethal effect on 3rd instar G. daurica larvae resulted in a deceleration of their growth and developmental processes and significantly increased the activity levels of CarE, GST and UGT. The detoxifying proteins encoded by up‐regulation genes may involve in the detoxification metabolism of chlorantraniliprole. Our results provide a basis for understanding the molecular mechanisms of chlorantraniliprole action and detoxification in this key grassland insect pest.

Sublethal Effects of Chlorantraniliprole on the Mobility Patterns of Sitophilus spp.: Implications for Pest Management

“…The insecticidal effectiveness and suppression of progeny by chlorantraniliprole have been previously evidenced for S. oryzae , exhibiting effectiveness in immediate and delayed mortality [ 33 , 34 ], along with its efficacy and persistence against S. zeamais when combined with lambda-cyhalothrin [ 35 ]. The sublethal effects of this promising compound have been studied for several coleopteran pests [ 36 , 37 , 38 , 39 , 40 , 41 , 42 ]. Nevertheless, the sublethal effects of chlorantraniliprole are yet to be determined for Sitophilus spp., among other significant stored-product pests.…”

Insecticide resistance: Monitoring, mechanism and management

“…Ren et al. (2024) evaluated the sublethal effects of chlorantraniliprole on the biological characteristics of Galeruca daurica .…”

“…Ren et al. (2024) found that the upregulated expression of partial CarEs, GST and UGT may participate in the detoxification metabolism of chlorantraniliprole in G. daurica . Yan et al.…”