Sublethal Effects of Copper Sulfate, Malathion and Paraquat on Protein Pattern of Oreochromis Niloticus

Sharaf-Eldeen, Khalid M.; Abdel-Hamid, Nassr-Allah

doi:10.21608/ejabf.2002.1745

Cited by 25 publications

(16 citation statements)

References 3 publications

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“…In the present study, the protein electrophoresis shows a large difference between reference and contaminated samples. This agrees with Sharaf-El-Deen & Abdel-Hamid (2002), who conclude that this difference is attributed to the production of a new DNA sequence, which synthesizes new protein bands. The study under review also agrees with several studies which report that the disappearance of bands is proved under the action of pollution (Muthukumaravel et al 2007;Osman et al 2010).…”

Section: Discussionsupporting

confidence: 91%

Vascular endothelial growth factor as early biomarker for hypoxia in Oreochromis niloticus

Hamdy

Shaban

Mahmoud

et al. 2018

Water & Environment J

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Aquatic pollution is a serious problem causing fish hypoxia. This study is conducted to assess the effects of environmental pollutants entering Lake Qaroun using vascular endothelial growth factor (VEGF) biomarker on Oreochromis niloticus. Water and fish samples were collected from contaminated sites to be compared with a reference relatively unpolluted site. Water physicochemical parameters, the oxidative stress biomarkers and histological studies of liver and gills were determined. Highly significant differences in water quality parameters were observed. Fish sampled from contaminated sites showed highly significant changes in oxidative stress and hypoxia‐related biomarkers compared to the reference site. These changes were accompanied by the existence of histological damages in liver and gill tissues. Moreover, there were differences in the banding pattern of fish serum proteins from the same sites. These results provide evidence that stress biomarkers and VEGF are useful for the assessment of hypoxia in aquatic environments influenced by multiple pollution sources.

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Section: Discussionsupporting

confidence: 91%

Vascular endothelial growth factor as early biomarker for hypoxia in Oreochromis niloticus

Hamdy

Shaban

Mahmoud

et al. 2018

Water & Environment J

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“…These parameters are closely related to the response of the animal to the environment, an indication that the environment where fish lives could exert some influence on the blood characteristics (Fernandes & Mazon 2003). As well as haematological parameters, also the determination of protein content in blood plasma is a good indicator to detect intra-and inter-specific variation among species, identify populations, and for investigation of ecological stress, physiological homeostasis and aquatic pollution (Sharaf-El-Deen & Abdel-Hamid 2002). Serum proteins are very complex and are involved in a wide range of physiological functions in both healthy and disease states, which is why they play a role of great importance for zoologists, enzymologists, immunologists and toxicologists.…”

Section: Introductionmentioning

confidence: 99%

Haematological and Biochemical Responses of Juvenile Malabar Blood Snapper (Lutjanus malabaricus Bloch & Schneider, 1801) Exposed to Different Rearing Temperatures and Diets

Mazumder¹

2019

JSM

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This study is conducted to examine the impacts of different rearing temperature and diet variation on haematological and serum biochemical parameters on the juvenile Lutjanus malabaricus, a commercially important fish species in were divided into eight treatments (4 temperatures × 2 diets) each with three replicates of five juveniles each. Fish were subjected to four different rearing temperatures (22, 26, 30 and 34°C) and fed with a commercial pellet and a natural shrimp diet. After 30 days of experimental period, blood samples were collected and transferred into two different tubes from each sample: One containing EDTA as an anticoagulant agent and the other without EDTA for serum collection. All the haematological and biochemical parameters in all the treatments were within the normal range. Changing temperature and diet led to a significant change (p<0.05) in the total length (TL), body weight (BW), red blood cell (RBC), haemoglobin (HB), haematocrit (HC), thrombocyte (TC) and white blood cell (WBC) but the change in mean corpuscular volume (MCV), mean cell haemoglobin (MCH) while mean cell haemoglobin concentration (MCHC) were not significant (p>0.05). The fish reared at 26°C and 30°C had significantly higher values than those reared at 22°C and 34°C (p<0.05). Total protein (TP), albumin (ALB), albumin/globin ration (A/G ratio), alkaline phosphatase (AP), aspartate aminotransferase (AST) and alanine transaminase (ALT) level were also significantly increased at 30°C and 26°C compared to that of 34°C and 22°C. Globulin (GLB) level showed the opposite trend. In all the cases the performances were higher in shrimp diet than pellet diet. It is suggested that these physiological parameters can be conveniently employed as health monitoring tools in fish culture practices. ABSTRAK Kajian ini dijalankan bagi meneliti impak perbezaan suhu dan variasi diet pada parameter hematologi dan serum biokimia pada juvenil Lutjanus malabaricus, satu spesies ikan komersial penting di Malaysia. Ikan (16-18 cm) dibahagikan kepada lapan rawatan (4 suhu × 2 diet) dan terdapat tiga replikat (5 ikan setiap replikat) bagi setiap rawatan. Ikan terdedah kepada empat suhu yang berbeza (22, 26, 30 dan 34°C) dan diberi makan pelet komersial dan diet udang semula jadi. Selepas 30 hari tempoh uji kaji, sampel darah diambil dan dipindahkan ke dalam dua tiub yang berbeza daripada setiap sampel: satu yang mengandungi EDTA sebagai agen antikoagulan dan yang lain tanpa EDTA untuk pengumpulan serum. Semua parameter hematologi dan biokimia dalam semua rawatan berada dalam julat normal. Perubahan suhu dan diet menyebabkan perubahan ketara (p <0.05) dalam jumlah panjang (TL), berat badan (BW), sel darah merah (RBC), hemoglobin (HB), hematokrit (HC), trombosit (TC) dan sel darah putih (WBC) tetapi perubahan jumlah min korpuskul (MCV), min sel hemoglobin (MCH), manakala nilai min kepekatan sel hemoglobin (MCHC) tidak signifikan (p > 0.05). Nilai yang lebih tinggi dilihat pada ikan yang diternak pada 26 dan 30°C daripada yang diternak pada 22 dan 34°C ...

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“…Several studies have been reported that Cu induce deleterious effects on physiological, haematological and histological changes in different fish species (Sharaf-Eldeen and Abdel-Hameid 2002;AbdelTawwab et al 2007;Carvalho and Fernandes 2008;Oliveira et al 2008). The haematological variables were frequently used as fast indicators of fish response to environmental stressors (Webener et al 1992;Shah 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of starving and feeding on some haematological and physiological responses of the Nile catfish, Clarias gariepinus exposed to copper at extreme seasons

Abdel-Hameid

2011

Fish Physiol Biochem

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The lethal concentration for 50% of fish for 96h (96h LC(50)) of copper (Cu(2+)) was estimated for the Nile catfish (Clarias gariepinus) in extreme seasons, winter and summer, 4.31 and 4.79 mg/l, respectively. The Nile catfish was exposed to 96h LC(50) of copper for 7 days in extreme winter and summer. The body indices, haematological parameters as well as some plasma and liver enzyme activities and metabolite level were significantly differed in fish exposed to copper over than those of the control fish. Most of the tested parameters were not significantly different between the control fish of winter and summer (winter, water temperature 18 ± 2°C and summer, 27 ± 2°C). The effect of two ration sizes on copper toxicity in two different seasons on C. gariepinus was justified. It was found that the haematological parameters and the tested plasma activities of enzymes were significantly valid due to season differences. The blood parameters as well as plasma activities of enzymes were significantly differed in fishes fed elevated ration (3%) and exposed to copper challenge. On the other hand, the exploit of low feeding ration (0.5%) along with copper exposure during the examined seasons induced non-significant differences of the tested parameters, from those of the corresponding control. Therefore, the low feeding ration provides some tolerance against the possible water-borne copper exposure.

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Sublethal Effects of Copper Sulfate, Malathion and Paraquat on Protein Pattern of Oreochromis Niloticus

Cited by 25 publications

References 3 publications

Vascular endothelial growth factor as early biomarker for hypoxia in Oreochromis niloticus

Vascular endothelial growth factor as early biomarker for hypoxia in Oreochromis niloticus

Haematological and Biochemical Responses of Juvenile Malabar Blood Snapper (Lutjanus malabaricus Bloch & Schneider, 1801) Exposed to Different Rearing Temperatures and Diets

Effect of starving and feeding on some haematological and physiological responses of the Nile catfish, Clarias gariepinus exposed to copper at extreme seasons

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