Sublethal effects of spinosad on some biochemical parameters of Xanthogaleruca luteola (Müller) (Coleoptera: Chrysomelidae)

Abstract

Mohammadzadeh Tamam B., Ghadamyari M., Sahragard A., Karimi-Malati A. (2014): Sublethal effects of spinosad on some biochemical parameters of Xanthogaleruca luteola (Müller) (Coleoptera: Chrysomelidae). Plant Protect. Sci., 50: 199-205.The effect of spinosad on some biochemical characteristics of Xanthogaleruca luteola was investigated. LC 30 and LC 50 of spinosad for the third instar larvae were determined as 1.6 and 2.9 ppm, respectively. Biochemical tests were carried out on the 3 rd instar larvae treated with LC 30 and LC 50 of spinosad. The LC 30 concentration decreased carbohydrate and glycogen content and increased lipid content significantly. There was a significant decrease in carbohydrate, glycogen, and protein contents following the exposure to LC 50 concentration. Available energy of the larvae exposed to LC 30 of spinosad increased significantly, whereas it decreased significantly at LC 50 . However, the energy consumed was increased at both concentrations significantly. Cellular energy allocation of larvae treated with LC 50 concentrations of spinosad decreased significantly. Glutathione-S-transferase and esterase activities were increased significantly at LC 50 . However, lipase activity was decreased at both concentrations.