Sublethal Toxicity Effects of P-CNB on POD and CAT Activity in Liver of Brocarded Carp

Abstract

Two kinds of antioxidant activities in liver of fish were investigated. Fish was exposed to different concentrations (1/6LC 50 , 1/4LC 50 , 1/3LC 50 , 1/2LC 50 ) and killed at 3, 7, 14, 21 and 28 day after exposure. The activities of Peroxidase (POD) and Catalase (CAT) in liver tissue were analyzed. The results indicated the activities of POD and CAT were induced from the third day in all treatment groups at post-exposures, they were higher than those of the control group (p＜0.01). The POD and CAT activities of the control group were unstable during 28-days. The relationship between CAT and POD activity is mutual compensation and it is stable under the pollution stress. The results demonstrated that the POD and CAT activities of liver inBrocarded Carp might be the promising biomarker of p-CNB exposure. Therefore it was suggested that the couple index of POD and CAT could be used as biomarker of nitroaromatics compounds, when their pollutions affected living beings in aquatic environment.

Study on Antioxidant Enzyme of Brocarded Carp as Biomarkers of 2,4-DNT Pollution in Water

“…Brocarded carp is a type of fish that has an important role in aquatic food chain. Because of this and other favorable trait such as being easily cultured in the laboratory, and high sensitivity to common toxicants [11][12][13] , it is employed in this study. Brocarded Carp was exposed to test solution containing 2,4-DNT with different concentrations.…”