Submerged culture conditions for the production of mycelial biomass and exopolysaccharides by the edible Basidiomycete Grifola frondosa

Lee, Bum Chun; Bae, Jun Tae; Pyo, Hyeong-Bae; Choe, Taeboo; Kim, Sang Woo; Hwang, Hye Jin; Yun, Jong Won

doi:10.1016/j.enzmictec.2003.12.015

Cited by 135 publications

(110 citation statements)

References 23 publications

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“…The high concentration of organic nitrogen mycelium allows or greater productivity, since this is necessary for the synthesis of proteins, nucleic acids and glucosamine as a major component of the cell wall. Mostly, the basidiomycetous fungi grow best in the presence of aminoacids (Leonowicz et al, 1990), differently inorganic nitrogen, when there was less exopolysaccharide and biomass. There are differences in the secretion and release of specific nitrogenase in the growth phase, the processing and use in source of nitrogen metabolism (Lee et al, 2004).…”

Section: Treatments Daysmentioning

confidence: 99%

“…Mostly, the basidiomycetous fungi grow best in the presence of aminoacids (Leonowicz et al, 1990), differently inorganic nitrogen, when there was less exopolysaccharide and biomass. There are differences in the secretion and release of specific nitrogenase in the growth phase, the processing and use in source of nitrogen metabolism (Lee et al, 2004). Nitrogen may also be a limiting factor for microbial growth, include bacteria (Farjalla, et al, 2006).…”

Section: Treatments Daysmentioning

confidence: 99%

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Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii

et al. 2015

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Lignocellulose is the most abundant environmental component and a renewable organic resource in soil. There are some filamentous fungi which developed the ability to break down and use cellulose, hemicellulose and lignin as an energy source. The objective of this research was to analyze the effect of three nitrogen resources (ammonium sulfate, saltpetre, soybean) in the holocellulolitic activity of Lentinula edodes EF 50 using as substrate sawdust E. benthamii. An experimental design mixture was applied with repetition in the central point consisting of seven treatments (T) of equal concentrations of nitrogen in ammonium sulfate, potassium nitrate and soybean. The enzymatic activity of avicelase, carboxymetilcellulase, β-glucosidase, xylanases and manganese peroxidase was determined. The humidity, pH, water activity (a w ) and qualitative analysis of mycelial growth in 8 times of cultivation were evaluated. The results showed negative effect on enzyme production in treatments with maximum concentration of ammonium sulfate and potassium nitrate. The treatments with cooked soybean flour expressed higher enzymatic activities in times of 3, 6 and 9 days of culture, except in the activity of manganese peroxidase. The highest production was observed in the treatment with ammonium sulfate, and soybean (83.86 UI.L -1 ) at 20 days of cultivation.

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Section: Treatments Daysmentioning

confidence: 99%

Section: Treatments Daysmentioning

confidence: 99%

Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii

et al. 2015

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“…On the other hand, the growth kinetics and the fungal morphology in broth fermentation are highly dependent on the culture conditions, such as carbon sources, C/N relation, initial pH and temperature, agitation intensity and aeration rate (20). A large amount of work has reported the effects of environmental parameters on the biomass concentration and on the yield of bioactive compounds by mushroom, but those researches are limited to few kinds of mushrooms (4,7,10,12,13,20,25).…”

Section: Introductionmentioning

confidence: 99%

“…Aiming at obtaining bioactive compounds from mushrooms, several researches have tried to cultivate medicinal mushrooms in solid artificial media for fruit body production rather than in submerged cultures for mycelial extract (13). However, submerged cultures have the potential for a higher mycelial production in a shorter period of time within a reduced space if compared to cultivation in solid artificial media.…”

Section: Introductionmentioning

confidence: 99%

Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Vieira¹,

Liebl²,

Tavares³

et al. 2008

Braz. J. Microbiol.

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Basidiomycete fungi of the Polyporus genus are a source of secondary metabolites which are of medicinal interest as antibacterial compounds. As these substances are produced in a small amount by the fungi, the study of the cultivation conditions in vitro that could possibly optimize their production seems of major importance. The effects of glucose and lactose, pH and agitation on biomass concentration and on the specific growth rate caused by the basidiomycete Polyporus tricholoma were investigated. The initial pH (4.5, 6.5 and 8.5) was autoregulated at pH 5.5, and the agitation increased the mycelial growth and the specific growth rate. The high concentration of carbon sources (4%) increased biomass production. The lactose concentration and the absence of agitation were determinant in the production of antibacterial metabolites. The characterization of the antibacterial substance by GC-MS indicated a major compound, isodrimenediol, produced by the fungus Polyporus tricholoma with activity against Staphylococcus aureus.

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“…Recently, some studies showed that the compositions of the growth medium can affect the specific rate of EPS synthesis. [12][13][14][15] The aim of present study was to find the optimum composition for submerged culture media of G. lucidum by changing the carbon and nitrogen source in tested media (Mushroom complete media), for the production of antioxidant and antimicrobial compound and analysis of the property of extracted compound by standard methods. …”

Section: Introductionmentioning

confidence: 99%

Optimization of fermentation media composition of ganoderma lucidum for improved production of antioxidant and antimicrobial compounds

Paliya¹,

Chaudhary²,

Verma³

et al. 2014

Phcog Commn.

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Submerged culture conditions for the production of mycelial biomass and exopolysaccharides by the edible Basidiomycete Grifola frondosa

Cited by 135 publications

References 23 publications

Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii

Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii

Submerged culture conditions for the production of mycelial biomass and antimicrobial metabolites by Polyporus tricholoma Mont.

Optimization of fermentation media composition of ganoderma lucidum for improved production of antioxidant and antimicrobial compounds

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