Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS

Cui, Meilin; Yang, Huanyi; He, Guoqing

doi:10.1631/jzus.b1500147

Cited by 17 publications

(14 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, Gorlenko et al (1959) established that pH values of 6.0-7.0 are ideal for increased mycelial mass in A. musiformis and A. conoides. Cui et al found that mycelial biomass and triterpenoid production in Ganoderma lucidum reached a maximum at pH 5.5, and declined rapidly outside the pH range of 4.5-6.5 (Cui et al 2015). This evidence indicates that some fungi prefer to grow in weakly acidic conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The most suitable concentrations of corn powder and soybean meal were 15 g/L and 6 g/L, respectively, in the present study. Further increase of the concentrations of these two substances was found to be unfavorable for mycelial growth and polysaccharide production, probably due to excessively high osmotic pressure caused by nutrients at high concentrations (Cui et al ).…”

Section: Discussionmentioning

confidence: 99%

“…Cui et al . found that mycelial biomass and triterpenoid production in Ganoderma lucidum reached a maximum at pH 5.5, and declined rapidly outside the pH range of 4.5–6.5 (Cui et al ). This evidence indicates that some fungi prefer to grow in weakly acidic conditions.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Armillariella tabescens mycelia on the growth performance and intestinal immune response and microflora of early‐weaned pigs

WenBin

Cheng

Tian

et al. 2017

Animal Science Journal

View full text Add to dashboard Cite

This study was performed to evaluate effects of Armillariella tabescens (A. tabescens) on the growth performance and intestinal immune response and microflora in early-weaned pigs when used as feed additive. A. tabescens mycelia were added to basal diets at concentrations of 0%, 0.1%, 0.3% or 0.9% (w/w). A total of 144 commercial cross-bred piglets were randomly allocated to one of these four diets and fed for 30 days. The growth performance of early-weaned piglets displayed improvement with diets containing 0.1% and 0.3% dried mycelia powder from A. tabescens. Supplementing with 0.1% or 0.3% A. tabescens mycelia induced a 2.6- and three-fold increase in secretory immunoglobulin A (sIgA) content in the jejunal mucosa, respectively, but had only a marginal effect on sIgA in the ileal mucosa. Expression of interleukin-2, interferon-γ, and tumor necrosis factor-α in the jejunal mucosa were elevated with A. tabescens mycelia administration. Increased amounts of Lactobacillus spp. and Bifidobacterium spp. in the jejunum, and decreased amounts of Escherichia coli in the jejunum and ileum were observed with the administration of A. tabescens-containing diets. This study demonstrated that A. tabescens had beneficial effects on the growth performance and intestinal microflora of early-weaned pigs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Armillariella tabescens mycelia on the growth performance and intestinal immune response and microflora of early‐weaned pigs

WenBin

Cheng

Tian

et al. 2017

Animal Science Journal

View full text Add to dashboard Cite

show abstract

“…The fermentation culture contained (g/L): wort 41, peptone 18.9, KH 2 PO 4 3, MgSO 4 1.5, and VB 1 0.05 (pH 5.4) (Cui et al, 2015).…”

Section: Liquid Cultivation Of Ganoderma Lucidummentioning

confidence: 99%

Apoptosis induction of colorectal cancer cells HTL-9 in vitro by the transformed products of soybean isoflavones by Ganoderma lucidum

Cui

Yang

2017

J. Zhejiang Univ. Sci. B

Self Cite

View full text Add to dashboard Cite

Soybean isoflavones have been one of the potential preventive candidates for antitumor research in recent years. In this paper, we first studied the transformation of soybean isoflavones with the homogenized slurry of Ganoderma lucidum. The resultant transformed products (TSI) contained (703.21±4.35) mg/g of genistein, with transformed rates of 96.63% and 87.82% of daidzein and genistein, respectively, and TSI also could enrich the bioactive metabolites of G. lucidum. The antitumor effects of TSI on human colorectal cancer cell line HTL-9, human breast cancer cell line MCF-7, and human immortalized gastric epithelial cell line GES-1 were also studied. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay showed that TSI could dramatically reduce the viability rates of HTL-9 cells and MCF-7 cells without detectable cytotoxicity on GES-1 normal cells when the TSI concentration was lower than 100 μg/ml. With 100 μg/ml of TSI, HTL-9 cells were arrested in the G1 phase, and late-apoptosis was primarily induced, accompanied with partial early-apoptosis. TSI could induce primarily earlyapoptosis by arresting cells in the G1 phase of MCF-7 cells. For HTL-9 cells, Western-blot and reverse-transcriptase polymerase chain reaction (RT-PCR) analysis showed that TSI (100 μg/ml) can up-regulate the expression of Bax, Caspase-3, Caspase-8, and cytochrome c (Cyto-c), indicating that TSI could induce cell apoptosis mainly through the mitochondrial pathway. In addition, the expression of p53 was up-regulated, while the expression of Survivin and nuclear factor κB (NF-κB) was down-regulated. All these results showed that TSI could induce apoptosis of HTL-9 cells by the regulation of multiple apoptosis-related genes.

show abstract

“…However, when more than one response must be optimized, it is necessary to use the so-called desirability functions [4]. RSM was often utilized to optimize different bioprocesses such as anaerobic digestion [5] and productions of bioethanol [6], lactic acid [7], triterpenoids [8], and biodiesel [9], among others. This methodology has also been successfully applied to xylose-to-xylitol bioconversion by the yeasts Candida tropicalis [10], Pachysolen tannophilus [11], Candida guilliermondii [12], and Debaryomyces hansenii [13].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Response Surface Methodology and Artificial Neural Network for Modeling Xylose‐to‐Xylitol Bioconversion

et al. 2016

View full text Add to dashboard Cite

Previous experimental data of xylose-to-xylitol bioconversion by Debaryomyces hansenii carried out according to a 33 full factorial design were used to model this process by two different artificial neural network (ANN) training methods. Models obtained for four responses were compared with those of response surface methodology (RSM). ANN models were shown to be superior to RSM in the predictive capacity, whereas the latter showed better performance in the generalization capability step. RSM with optimization using a genetic algorithm was revealed as a whole to be the best modeling option, which suggests that the comparative performances of RSM and ANN may be a highly problem-specific issue

show abstract

Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS

Cited by 17 publications

References 36 publications

Effects of Armillariella tabescens mycelia on the growth performance and intestinal immune response and microflora of early‐weaned pigs

Effects of Armillariella tabescens mycelia on the growth performance and intestinal immune response and microflora of early‐weaned pigs

Apoptosis induction of colorectal cancer cells HTL-9 in vitro by the transformed products of soybean isoflavones by Ganoderma lucidum

Comparison of Response Surface Methodology and Artificial Neural Network for Modeling Xylose‐to‐Xylitol Bioconversion

Contact Info

Product

Resources

About