Submergence-responsive MicroRNAs are Potentially Involved in the Regulation of Morphological and Metabolic Adaptations in Maize Root Cells

Zhang, Zuxin; Wei, Liya; Zou, Xilin; Tao, Yuezhi; Liu, Zhijie; Zheng, Yanyan

doi:10.1093/aob/mcn129

Cited by 152 publications

(133 citation statements)

References 59 publications

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“…Several miRNA levels were differentially regulated in maize seedlings exposed to submergence, where miR167, miR166, miR171, and miR396 were induced during the early phase of submergence. On the other hand, miR159, miR474, and miR528 were downregulated during the early phase but induced after long-term exposure (Zhang et al, 2008). Interestingly, the numbers of the miR169 family were upregulated in rice and Arabidopsis after salt stress (Zhao et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Identification of UV-B-induced microRNAs in wheat

Wang¹,

Sun²,

Song³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. MicroRNAs (miRNAs) play critical roles in posttranscriptional gene regulation and act as important endogenous regulators to various stressors. Ultraviolet-B (UV-B) radiation is a major factor influencing crop growth and development. In this study, we isolated a novel wheat miRNA, named Tae-miR6000, and confirmed its expression diversity after UV-B treatments. Additionally, using the Northern blotting technique, we found that six miRNAs were highly responsive to UV-B stress in wheat. Of these six miRNAs, miR159, miR167a, and miR171 were significantly upregulated, and the remaining three miRNAs were downregulated, at different time points after UV-B treatment. This result indicates that miRNAs may be involved in the regulation of targets after induction by UV-B stress. Furthermore, promoter analysis of the UV-B-responsive miRNA genes revealed some light-relevant cis-elements, such as the I-box and G-box. Taken together, the results of this study suggest that wheat miRNAs play important roles in the response to UV-B stress.

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Section: Discussionmentioning

confidence: 99%

Identification of UV-B-induced microRNAs in wheat

Wang¹,

Sun²,

Song³

et al. 2013

Genet. Mol. Res.

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“…Phytohormone treatments induced the expression of several miRNAs in rice (Liu et al, 2009). Predicted targets for several miRNAs encode WRKY factors (Zhang et al, 2008b; S.P. Pandey and I.T.…”

Section: Regulation Via Other Tfs and Proteinsmentioning

confidence: 99%

The Role of WRKY Transcription Factors in Plant Immunity

2009

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“…Recently, an evolutionarily conserved monocotspecific miRNA, miR528, was identified to respond to multiple stresses, including salt, drought, low-temperature, submergence, nitrate starvation, and arsenite and arsenate stresses in maize (Zea mays), sugarcane (Saccharum officinarum), phalaenopsis orchid (Phalaenopsis aphrodite), and rice (Oryza sativa; Zhang et al, 2008;Xu et al, 2011;Ferreira et al, 2012;Nischal et al, 2012;Sharma et al, 2015). In addition, deep sequencing analysis shows that miR528 responds to diazotrophic bacteria in maize and might be involved in cytoplasmic male sterility in rice anther (Thiebaut et al, 2014;Yan et al, 2015).…”

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confidence: 99%

Constitutive Expression of Rice MicroRNA528 Alters Plant Development and Enhances Tolerance to Salinity Stress and Nitrogen Starvation in Creeping Bentgrass

et al. 2015

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MicroRNA528 (miR528) is a conserved monocot-specific small RNA that has the potential of mediating multiple stress responses. So far, however, experimental functional studies of miR528 are lacking. Here, we report that overexpression of a rice (Oryza sativa) miR528 (Osa-miR528) in transgenic creeping bentgrass (Agrostis stolonifera) alters plant development and improves plant salt stress and nitrogen (N) deficiency tolerance. Morphologically, miR528-overexpressing transgenic plants display shortened internodes, increased tiller number, and upright growth. Improved salt stress resistance is associated with increased water retention, cell membrane integrity, chlorophyll content, capacity for maintaining potassium homeostasis, CATALASE activity, and reduced ASCORBIC ACID OXIDASE (AAO) activity; while enhanced tolerance to N deficiency is associated with increased biomass, total N accumulation and chlorophyll synthesis, nitrite reductase activity, and reduced AAO activity. In addition, AsAAO and COPPER ION BINDING PROTEIN1 are identified as two putative targets of miR528 in creeping bentgrass. Both of them respond to salinity and N starvation and are significantly down-regulated in miR528-overexpressing transgenics. Our data establish a key role that miR528 plays in modulating plant growth and development and in the plant response to salinity and N deficiency and indicate the potential of manipulating miR528 in improving plant abiotic stress resistance.Abiotic stresses, especially drought, salt, and nitrogen (N) deficiency, are limiting factors for plant growth, development, and agricultural productivity. To cope with drought and salt stresses, plants have evolved similar strategies of osmotic adjustment (Munns, 2002;Zhu, 2002). Plants also evolved salinity-specific adjustments against ionic disequilibrium, which encompass excluding salt entry into plants and compartmentalizing ions into vacuoles or old leaves (Munns, 1993;Yeo, 1998). Another worldwide limiting factor for crop yields is N deficiency, which triggers reduced leaf growth rate and photosynthetic rate (Chapin et al., 1988). Due to the sessile nature of plants, abiotic stresses are unavoidable. Therefore, it is critical to develop reliable procedures to genetically modify plants for improved performance under environmental stresses, thereby enhancing agricultural productivity to meet the ever-growing demands in food production.Genetic engineering plays an increasingly important role in agronomic trait modifications in crop species. Currently, many genes encoding functional proteins, transcription factors, and proteins involved in signaling pathways have been identified as abiotic stressresponsive genes (Shinozaki and Yamaguchi-Shinozaki, 2007;Masclaux-Daubresse et al., 2010;Turan et al., 2012). Constitutive expression of some of these genes in transgenic plants has been demonstrated to lead to enhanced salt or drought tolerance (Golldack et al., 2011;Kim et al., 2013;Lu et al., 2013;Li et al., 2014). However, details of the regulatory network in the plant...

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Submergence-responsive MicroRNAs are Potentially Involved in the Regulation of Morphological and Metabolic Adaptations in Maize Root Cells

Abstract: Submergence-responsive miRNAs are involved in the regulation of metabolic, physiological and morphological adaptations of maize roots at the post-transcriptional level.

Cited by 152 publications

References 59 publications

Identification of UV-B-induced microRNAs in wheat

Identification of UV-B-induced microRNAs in wheat

The Role of WRKY Transcription Factors in Plant Immunity

Constitutive Expression of Rice MicroRNA528 Alters Plant Development and Enhances Tolerance to Salinity Stress and Nitrogen Starvation in Creeping Bentgrass

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