Submicrometer Imaging by Laser Ablation-Inductively Coupled Plasma Mass Spectrometry via Signal and Image Deconvolution Approaches

Malderen, Stijn J. M. Van; Elteren, Johannes T. van; Vanhaecke, Frank

doi:10.1021/acs.analchem.5b00700

Cited by 52 publications

(56 citation statements)

References 51 publications

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“…They can overpass the limited accessibility of large facilities, maintaining a comparable spatial resolution [9,10]. Over the last few years, many efforts have been made to perform nanometer spatial resolution imaging in the EUV and SXR spectral ranges employing both large scale and compact sources.…”

Section: Introduction and Previous Achievementsmentioning

confidence: 99%

Bioimaging Using Full Field and Contact EUV and SXR Microscopes with Nanometer Spatial Resolution

et al. 2017

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We present our recent results, related to nanoscale imaging in the extreme ultraviolet (EUV) and soft X-ray (SXR) spectral ranges and demonstrate three novel imaging systems recently developed for the purpose of obtaining high spatial resolution images of nanoscale objects with the EUV and SXR radiations. All the systems are based on laser-plasma EUV and SXR sources, employing a double stream gas puff target. The EUV and SXR full field microscopes-operating at 13.8 nm and 2.88 nm wavelengths, respectively-are currently capable of imaging nanostructures with a sub-50 nm spatial resolution with relatively short (seconds) exposure times. The third system is a SXR contact microscope, operating in the "water-window" spectral range (2.3-4.4 nm wavelength), to produce an imprint of the internal structure of the investigated object in a thin surface layer of SXR light sensitive poly(methyl methacrylate) photoresist. The development of such compact imaging systems is essential to the new research related to biological science, material science, and nanotechnology applications in the near future. Applications of all the microscopes for studies of biological samples including carcinoma cells, diatoms, and neurons are presented. Details about the sources, the microscopes, as well as the imaging results for various objects will be shown and discussed.

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Section: Introduction and Previous Achievementsmentioning

confidence: 99%

Bioimaging Using Full Field and Contact EUV and SXR Microscopes with Nanometer Spatial Resolution

et al. 2017

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“…Laser-produced plasma sources, emitting short wavelength radiation in the SXR and EUV spectral ranges offer an important alternative to be sources dedicated for compact imaging systems, allowing to overpass the limited accessibility of large facilities and maintaining a comparable spatial resolution 5,6 . So far, many efforts have been made to perform nanometer spatial resolution imaging in the EUV and SXR spectral ranges over the last few years employing both large scale and compact sources.…”

Section: Introductionmentioning

confidence: 99%

Soft x-ray imaging with incoherent sources

et al. 2017

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In this work we present experimental, compact desk-top SXR microscope, the EUV microscope which is at this stage a technology demonstrator, and finally, the SXR contact microscope. The systems are based on laser-plasma EUV and SXR sources, employing a double stream gas puff target. The EUV and SXR full field microscopes, operating at 13.8 nm and 2.88 nm wavelengths, respectively, are capable of imaging nanostructures with a sub-50 nm spatial resolution with relatively short (seconds) exposure times. The SXR contact microscope operates in the "water-window" spectral range, to produce an imprint of the internal structure of the sample in a thin layer of SXR light sensitive photoresist. Applications of such desk-top EUV and SXR microscopes for studies of variety of different samples -test objects for resolution assessment and other objects such as carbon membranes, DNA plasmid samples, organic and inorganic thin layers, diatoms, algae and carcinoma cells, are also presented. Details about the sources, the microscopes as well as the imaging results for various objects will be presented and discussed. The development of such compact imaging systems may be important to the new research related to biological, material science and nanotechnology applications.

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“…Over-sampling of the material can be employed to further improve spatial resolution beyond the smallest available spot size on commercial ablation systems. 25 A consideration of increasingly smaller sampling areas is that if the transport efficiency and response of the instrumental detector remain constant, the inherently small mass resulting from higher spatial resolutions results in a poorer signal-to-noise (SN) ratio and a worse limit of detection (LOD). This can limit the applicability of LA-ICP-MS when analysing ultra-trace elements in biological materials or isotope ratios in single particles, unless an improvement in the absolute sensitivity of the system can be achieved.…”

Section: Introductionmentioning

confidence: 99%

High-Speed, Integrated Ablation Cell and Dual Concentric Injector Plasma Torch for Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

et al. 2015

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In recent years, laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) has gained increasing importance for biological analysis, where ultratrace imaging at micrometer resolution is required. However, while undoubtedly a valuable research tool, the washout times and sensitivity of current technology have restricted its routine and clinical application. Long periods between sampling points are required to maintain adequate spatial resolution. Additionally, temporal signal dispersion reduces the signal-to-noise ratio, which is a particular concern when analyzing discrete samples, such as individual particles or cells. This paper describes a novel, two-volume laser ablation cell and integrated ICP torch designed to minimize aerosol dispersion for fast, efficient sample transport. The holistic design utilizes a short, continuous diameter fused silica conduit, which extends from the point of ablation, through the ICP torch, and into the base of the plasma. This arrangement removes the requirement for a dispersive component for argon addition, and helps to keep the sample on axis with the ICP cone orifice. Hence, deposition of sample on the cones is theoretically reduced with a resulting improvement in the absolute sensitivity (counts per unit mole). The system described here achieved washouts of 1.5, 3.2, and 4.9 ms for NIST 612 glass, at full width half, 10%, and 1% maximum, respectively, with an 8–14-fold improvement in absolute sensitivity, compared to a single volume ablation cell. To illustrate the benefits of this performance, the system was applied to a contemporary bioanalytical challenge, specifically the analysis of individual biological cells, demonstrating similar improvements in performance.

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Submicrometer Imaging by Laser Ablation-Inductively Coupled Plasma Mass Spectrometry via Signal and Image Deconvolution Approaches

Cited by 52 publications

References 51 publications

Bioimaging Using Full Field and Contact EUV and SXR Microscopes with Nanometer Spatial Resolution

Bioimaging Using Full Field and Contact EUV and SXR Microscopes with Nanometer Spatial Resolution

Soft x-ray imaging with incoherent sources

High-Speed, Integrated Ablation Cell and Dual Concentric Injector Plasma Torch for Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

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