Submicrometer Plate Heights for Capillaries Packed with Silica Colloidal Crystals

Malkin, Douglas S.; Wei, Bingchuan; Fogiel, Arthur J.; Staats, Sau Lan; Wirth, Mary J.

doi:10.1021/ac100062t

Cited by 48 publications

(82 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While there has not been a study of the role of order in dc field separation in a colloidal crystal, a study of long DNA separation under a pulsed electric field in a 3D colloidal array found that an ordered matrix improved the separation resolution over a disordered matrix [43]. We anticipate that dc electric field separations in 3D colloidal arrays will also benefit from increased array order, similar to the results for protein chromatography in colloidal crystals [44].…”

Section: Discussionsupporting

confidence: 59%

Experimental study of the effect of disorder on DNA dynamics in post arrays during electrophoresis

Olson

Dorfman

2012

Phys. Rev. E

View full text Add to dashboard Cite

We used top-down fabrication techniques to create both an ordered hexagonal array and a disordered array of 1 µm diameter cylindrical posts in a silicon dioxide microchannel with the same number of posts per unit area. The electrophoretic mobility and dispersion coefficient of λ DNA in each of the arrays were obtained as a function of the electric field using ensembles of DNA molecules in a double channel device that minimizes experimental artifacts. To deepen our understanding of the transport, we also used fluorescence microscopy to examine the dynamics of single DNA molecules as they interact with the arrays at a fixed value of the electric field. Based on the results of these two types of experiments, we conclude that the electrophoretic mobility is not dependent on the array order but that band broadening in the device is greater in the disordered array.

show abstract

Section: Discussionsupporting

confidence: 59%

Experimental study of the effect of disorder on DNA dynamics in post arrays during electrophoresis

Olson

Dorfman

2012

Phys. Rev. E

View full text Add to dashboard Cite

show abstract

“…A typical SEM image is shown in Fig. 1B, confirming that the packing of silica particles is disordered, in contrast to our earlier work that shows (111) or (100) crystalline packing [19]. …”

Section: Resultssupporting

confidence: 78%

Protein UTLC-MALDI–MS using thin films of submicrometer silica particles

Zhang

Ratnayaka

Wirth

2011

Journal of Chromatography A

Self Cite

View full text Add to dashboard Cite

Slides for ultra thin-layer chromatography (UTLC) slides were made by coating nonporous silica particles, chemically modified with polyacrylamide, as 15 μm films on glass or silicon. Three proteins, myoglobin, cytochrome c and lysozyme, are nearly baseline resolved by the mechanism of hydrophilic interaction chromatography. A plate height as low as 3 μm, with 3,900 plates, are observed in 14 mm. Varying silica particle diameter among 900, 700 and 350 nm showed that decreasing particle diameter slightly improves resolution but slows the separation. Matrix-assisted laser desorption/ionization (MALDI)-MS of the proteins after separation is demonstrated by wicking sufficient sinapinic acid into the separation medium.

show abstract

“…A very highly ordered silica colloidal crystal of 0.4×0.4 mm 2 has been made and was shown to separate DNA fragments by biased reptation 23. Packed inside capillaries, silica colloidal crystals have been demonstrated to give plate heights as low as 100 nm in the electrochromatography of a fluorescent dye, when corrected for injected width 24. The corrected plate height was contributed entirely by diffusion, within experimental error.…”

Section: Introductionmentioning

confidence: 82%

Plate Heights below 50 nm for Protein Electrochromatography Using Silica Colloidal Crystals

2010

Self Cite

View full text Add to dashboard Cite

Silica colloidal crystals formed from 330 nm nonporous silica spheres inside of 75-μm i.d. fused silica capillaries were evaluated for the efficiency of capillary electrochromatography of proteins. Three proteins, ribonuclease A, cytochrome C and lysozyme, each covalently labeled with fluorophor, were well separated over a distance of 1 cm by isocratic electromigration, using 40:60 acetonitrile:water with 0.1% formic acid. A van Deemter plot showed that the plate height for lysozyme, which was the purest of the three proteins, was diffusion-limited for electric fields ranging from 400 to 1400 V/cm. The plate height for lysozyme was below 50 nm at almost all of the migration velocities, and it approached 10 nm at the highest velocity. Eddy diffusion was negligible. Lysozyme migrated over a 12 mm separation length with more than 10 6 plates in 1.5 minutes. These results indicate that silica colloidal crystals are well suited for electrically driven separations of large, highly charged analytes such as proteins. The 10 6 plates observed for a separation length of barely more than a cm means they are potentially valuable for miniaturized separations in microchip and μTAS devices.

show abstract

Submicrometer Plate Heights for Capillaries Packed with Silica Colloidal Crystals

Cited by 48 publications

References 18 publications

Experimental study of the effect of disorder on DNA dynamics in post arrays during electrophoresis

Experimental study of the effect of disorder on DNA dynamics in post arrays during electrophoresis

Protein UTLC-MALDI–MS using thin films of submicrometer silica particles

Plate Heights below 50 nm for Protein Electrochromatography Using Silica Colloidal Crystals

Contact Info

Product

Resources

About