Submicroscopic Structure of Elastic Fibres as Observed in the Polarization Microscope

Romhányi, G

doi:10.1038/182929a0

Cited by 24 publications

(5 citation statements)

References 5 publications

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“…studies on elastic tissue of various kinds have shown that elastic tissue is closely related to the collagen moiety of this tissue. Romhanyi (12) showed that the protein filaments embedded in the matrix of the elastic fibres are in close structural relation to the collagen-type Frotein, an observation which is supported by electron microscope studies of Karrer on the aorta (13), and on the tunica propria of bronchioles (14), where collagen fibrils appear at the periphery of and are partially embedded in the homogeneous elastic component. Unpublished observations, by one of us (H.S.…”

Section: Figurementioning

confidence: 83%

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Studies on Cartilage

Sheldon

Robinson

1960

The Journal of Cell Biology

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Electron microscope observations on rabbit ear cartilage following the administration of papain show that both the elastic component of the matrix and the amorphous material disappear leaving a matrix which consists of delicate fibrils which are presumed to be collagen. This unmasking of fibrils coincides with the appearance of an abnormal component in the electrophoretic pattern of the rabbit's serum. The chondrocytes show vacuoles in their cytoplasm which appear at the same time that the cells appear crenated in the light microscope. A ruffly appearance of the cell surface membrane coincides with this vacuolization, and vacuoles often appear open and in continuity with the extracellular space. The resurgence of the rabbit ear is accompanied by a reconstitution of both the amorphous material and the elastic component of the matrix. During this period numerous dilated cisternae of the endoplasmic reticulum which contain a moderately dense material are present in the chondrocyte cytoplasm. We have been unable to demonstrate a direct relationship between the elastic component of the matrix and a particular component of the chondrocyte cytoplasm, but it is clear that changes occur in the cartilage cell cytoplasm during both the depletion and reconstitution of the matrix. Previous studies on the effect of papain on elastic tissue are noted and the possible relationships between changes in the cells and matrix of this elastic cartilage are discussed.The phenomenon of collapse of the ears of young rabbits after papain is administered intravenously was observed by Thomas (1) who reported the reversibility of this phenomenon. He described the appearance of auricular and other cartilage in the light microscope during the phase of collapse, and commented on a loss of basophilia in the cartilage matrix. Further studies on this phenomenon have been reported by Spicer and Bryant (2, 3), McCluskey and Thomas (4), Tsahas (5), and Bryant, Leder, and Stetten (6), who describe the appearance of an abnormal acid component in the electrophoretic pattern of serum after the administration of papain. The present authors thought that this phenomenon of collapse and recovery might prove suitable for an electron microscope

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Section: Figurementioning

confidence: 83%

“…of 1 per cent solution of crude papain 1 was administered intravenously to 12 young rabbits each of which weighed less than 1000 grams. Specimens of ear cartilage were removed 30 minutes, 2,4,8,12,18,24, and 48 hours later. To avoid the effects of trauma no more than 2 interval biopsies were performed on any one ear.…”

Section: Materials and Metitodsmentioning

confidence: 99%

Studies on Cartilage

Sheldon

Robinson

1960

The Journal of Cell Biology

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“…We have introduced three different indices for quantifying metachromasia based on the color of digital microscopic images. Since previous quantitative measures of metachromasia are based on comparison of optical densities at two different wavelengths (Hattori et al, 1996) or at two different polarization angles (Heuft & Bohm, 1978;Romhanyi, 1963), they are not easily applied under conditions available in ordinary cell biological laboratories. Since the novel indices are based on quantifying color, a brief introduction to the RGB color model is required.…”

Section: Chondrogenic Cell Suspension Samples (Seementioning

confidence: 99%

Isolation and Micromass Culturing of Primary Chicken Chondroprogenitor Cells for Cartilage Regeneration

et al. 2023

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Much of the skeletal system develops by endochondral ossification, a process that takes place in early fetal life. This makes the early stages of chondrogenesis, i.e., when chondroprogenitor mesenchymal cells differentiate to chondroblasts, challenging to study in vivo. In vitro methods for the study of chondrogenic differentiation have been available for some time. There is currently high interest in developing fine‐tuned methodology that would allow chondrogenic cells to rebuild articular cartilage and restore joint functionality. The micromass culture system that relies on embryonic limb bud‐derived chondroprogenitor cells is a popular method for the study of the signaling pathways that control the formation and maturation of cartilage. In this protocol, we describe a technique fine‐tuned in our laboratory for culturing limb bud‐derived mesenchymal cells from early‐stage chick embryos in high density (Basic Protocol 1). We also provide a fine‐tuned method for high‐efficiency transient transfection of cells before plating using electroporation (Basic Protocol 2). In addition, protocols for histochemical detection of cartilage extracellular matrix using dimethyl methylene blue, Alcian blue, and safranin O are also provided (Basic Protocol 3 and Alternate Protocols 1 and 2, respectively). Finally, a step‐by‐step guide on a cell viability/proliferation assay using MTT reagent is also described (Basic Protocol 4). © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Micromass culture of chick embryonic limb bud‐derived cells Basic Protocol 2: Transfection of cells with siRNA constructs using electroporation prior to micromass culturing Basic Protocol 3: Qualitative and quantitative assessment of cartilage matrix production using dimethyl methylene blue staining and image analysis Alternate Protocol 1: Qualitative assessment of cartilage matrix production using Alcian blue staining Alternate Protocol 2: Qualitative assessment of cartilage matrix production using safranin O staining Basic Protocol 4: Measurement of mitochondrial activity with the MTT assay

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“…Меж тем, круг подобных явлений достаточно широк, чтобы привести к разработке на элементарной технической базе ХХ века (к которой относятся первые «морфологические» реализации поляризационно-микроскопических принципов измерений) поляризационных и поляризационно-интерференционных насадок для CLEM. Примерами явлений, структур и реактивных феноменов такого рода, демонстрирующими разнообразие приложений этой техники, являются прецеденты поляризационно-микроскопического измерения мембран и гигантских везикулярных структур [17], включая анализ текучести мембран (последнее -на флуоресцентном / люминесцентном поляризационном микроскопе) и, вследствие этого, деформируемости мембранных цитологических структур [18]; исследования и измерений длины саркомера на мышечном препарате [19] и изменений свойств эластичных волокон с субмикроскопической прецизионностью [20,21], двулучепреломления клеточных струк-…”

Section: Introductionunclassified

Towards Polarizing Correlative Light-Electron Microscopy (PCLEM).

Градов¹

2018

Morphologia

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Шановні колеги! У рубриці "Методологія наукових досліджень" редакція продовжує публікацію матеріалів, що пов'язані з найважливішими аспектами наукової і навчальної діяльності: організаційно-методичним забезпеченням періодичних наукових видань, загальними принципами біостатистичного та математичного супроводження досліджень, а також оригінальними методичними підходами вітчизняних і зарубіжних морфологів.

show abstract

Submicroscopic Structure of Elastic Fibres as Observed in the Polarization Microscope

Cited by 24 publications

References 5 publications

Studies on Cartilage

Studies on Cartilage

Isolation and Micromass Culturing of Primary Chicken Chondroprogenitor Cells for Cartilage Regeneration

Towards Polarizing Correlative Light-Electron Microscopy (PCLEM).

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