Submitochondrial distribution and delayed proteolysis of subunit c of the H+-transporting ATP-synthase in ovine ceroid-lipofuscinosis

Hughes, Stephanie M.; Moroni-Rawson, Pisana; Jolly, R. D.; Jordan, T. William

doi:10.1002/1522-2683(200105)22:9<1785::aid-elps1785>3.0.co;2-l

Cited by 14 publications

(14 citation statements)

References 36 publications

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“…Protein spots for matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analysis were prepared as described previously (36). Excised gel spots (approximately 1 to 2 mm 3 ) were destained for 45 min in 100 l of 50% (vol/vol) acetonitrile (ACN)-50 mM NH 4 HCO 3 and then dehydrated with two washes in 100% ACN and dried by vacuum centrifugation.…”

supporting

confidence: 57%

Differential Liver Protein Expression during Schistosomiasis

2007

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The arrival of eggs in the liver during Schistosoma mansoni infection initiates a protective granulomatous response; however, as the infection progresses, this response results in chronic liver fibrosis. To better understand the impact of schistosomiasis on liver function, we used a proteomic approach to identify proteins whose expression was significantly altered in schistosome-infected mice 8 weeks postinfection. Identification of differentially expressed proteins by mass fingerprinting revealed that schistosome infection markedly reduced the abundance of proteins associated with several normal liver functions (i.e., citric acid cycle, fatty acid cycle, and urea cycle), while proteins associated with stress responses, acute phase reactants, and structural components were all significantly more abundant. The expression patterns of several immunity-related proteins (peroxiredoxin 1, arginase 1, and galectin 1) suggested that different protein forms are associated with schistosome infection. These findings indicate that acute schistosomiasis has a significant impact on specific liver functions and, moreover, that the alterations in specific protein isoforms and upregulation of unique proteins may be valuable as new markers of disease.

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supporting

confidence: 57%

Differential Liver Protein Expression during Schistosomiasis

2007

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“…Protein spots on Coomassie Brilliant Blue R-350-stained gels were excised and washed three times with ultrapure water and subjected to tryptic digestion using a modified procedure of Hughes et al (Hughes et al 2001). The gel pieces containing proteins were destained twice with 25 mM NH 4 HCO 3 in 50% (V/V) acetonitrile (ACN), dried with a centrifugal concentrator (Thermo Savant SpeedVac Concentrator, USA).…”

Section: Protein Identification By Msmentioning

confidence: 99%

Proteomic Analysis of Interactions Between the Generalist Herbivore Spodoptera exigua (Lepidoptera: Noctuidae) and Arabidopsis thaliana

et al. 2009

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In this study, comparative proteomics was used to investigate the interaction of Spodoptera exigua and Arabidopsis thaliana. By using 2-D electrophoresis of differentially expressed proteins, combined with high-throughput matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and MALDI-TOF/ TOF MS, the changes in the abundance of proteins induced by insect feeding were studied in A. thaliana. More than 1,100 protein spots were reproducibly detected on each gel. The intensities of 30 protein spots in particular changed significantly, showing differences in volume of at least twofold. Among these, 17 protein spots were upregulated, and 13 were downregulated following an 8-h insect feeding period. Nineteen insect-feeding-responsive proteins were identified, all of which were involved in metabolic regulation, binding functions or cofactor requirement of protein, cell rescue, and defense and virulence, as assessed by Munich Information Center for Protein Sequences function category. About 50% of these were involved in metabolism, including transketolase, S-adenosylmethionine synthase 3, 2,3-biphosphoglycerate-independent phosphoglycerate mutase, beta-ureidopropionase, GDP-Dmannose 3′,5′-epimerase, and fatty acid synthase. The identification of insect-feeding-responsive proteins on Arabidopsis provides not only new insights into insect stress but also a good start for further investigation of their functions. Understanding how the plant responses to insects in the proteomic level will provide tools for a better management of insect pest in the field.

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“…Image analysis and quantifi cation of individual amounts of protein were performed using ImageMaster 2-D Platinum v5.0 (Amersham Biosciences) software. Identifi cation of proteins was carried out as previously described (Hughes et al 2001), using MALDI mass fi ngerprinting of tryptic digests of excised protein spots. The spectra were matched to sequences in the National Centre for Biotechnology Information non-redundant database using two algorithms, ProFound (http://prowl.rockefeller.edu/profound.bin/WebProFound.exe) and XProteo (http://xproteo.com:2698/).…”

Section: Proteomicsmentioning

confidence: 99%

Segmental axonopathy of Merino sheep in New Zealand

Jolly

Johnstone

Williams³

et al. 2006

New Zealand Veterinary Journal

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The disorder is the same as that described in Merinos in Australia as segmental axonopathy, and believed to have an inherited aetiology. The lesions and protein changes indicate abnormalities of the cytoskeleton, its relationship with the myelin sheath, and myosin Va molecular motor. The consequence appears to be abnormal axonal transport and inability to maintain the integrity of axons and their myelin sheaths.

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Submitochondrial distribution and delayed proteolysis of subunit c of the H+-transporting ATP-synthase in ovine ceroid-lipofuscinosis

Cited by 14 publications

References 36 publications

Differential Liver Protein Expression during Schistosomiasis

Differential Liver Protein Expression during Schistosomiasis

Proteomic Analysis of Interactions Between the Generalist Herbivore Spodoptera exigua (Lepidoptera: Noctuidae) and Arabidopsis thaliana

Segmental axonopathy of Merino sheep in New Zealand

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