Subnanogram detection oft‐butyldimethylsilyl fatty acid esters by mass fragmentography

Abstract

The mass spectra of t-butyldimethylsilyl fatty acid esters all display a pronounced (M-C4H9)+ ion. The proportion of the total ionization carried by this fragment, particularly for saturated and mono-, di-, and tri-unsaturated acid derivatives, facilitates their qualitative analysis at the subanogram level by mass fragmentography.

Synthesis and characterization of [1‐13C]‐and d8‐arachidonic acid

“…were consistent with data reported by Phillipou et al (12). As shown in Figures 1 and 2, the t-BDMS ester of 13C-and ds-arachidonic acid also displayed the most intense [M-57] + peaks at high mass, m/e 362 and m/e 369, respectively.…”

Microdetermination of molecular species of oligo- and polyunsaturated diacylglycerols by gas chromatography-mass spectrometry of their tert-butyldimethylsilyl ethers

“…Figure 2 gives the mass spectrum recorded for 1-palmitoyl-2-eicosapentaenoyl-sn-glycerol. Many of the fragments are similar to those reported for the diacylglycerol TMS ethers (4, 8), but the most important feature is the high abundance peak corresponding to (M -57)+ that is also characteristic of the t-BDMS spectra of steroids and prostaglandins (13) and of free fatty acids (21). The high intensity of the (M -57)+ ion allows the ready determination of carbon number and degree of unsaturation even for species containing docosahexaenoic acid (22:6).…”

Sterically crowded trialkylsilyl derivatives for chromatography and mass spectrometry of biologically-important compounds

“…Recently, the tert-butyldimethylsilyl (TBDMSi) group has been employed for GC, SIM, and MS of prostaglandins (7,8), steroids (9)(10)(11)(12)(13)(14), carbohydrates (15)(16)(17), fatty acids (18), and nucleosides (19)(20)(21). This group forms one member of a series of "sterically crowded trialkylsilyl" (SCTASi) groups which we have been studying.…”