Subpollen particles are rich carriers of major short ragweed allergens and <scp>NADH</scp> dehydrogenases: quantitative proteomic and allergomic study

Smiljanić, Katarina; Apostolović, Danijela; Trifunović, S.; Ognjenović, Jana; Peruško, Marija; Mihajlovic, Luka; Burazer, Lidija; Hage, Marianne van; Veličković, Tanja Ćirković

doi:10.1111/cea.12874

Cited by 26 publications

(18 citation statements)

References 43 publications

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“…A substantial proportion of fungal spores is small enough to enter the lower airways and common allergens are found in the respirable fine particle fraction (<3 μm) ( 40 ). While pollen grains themselves are large (10–200 μm) ( 41 ), and only few can reach the lower airways, grass, weed and birch pollen allergens associated with particles under 5 μm (starch grains, subpollen particles) have been shown to be released from the pollen grain after light rainfall ( 42 – 45 ). They can furthermore occur in association with smaller airborne fractions such as suspended particulate material deriving from industrial combustion and vehicle exhaust emissions ( 46 , 47 ).…”

Section: The Role Of Particulates In Allergic Sensitization—what Are mentioning

confidence: 99%

Mechanisms of Particles in Sensitization, Effector Function and Therapy of Allergic Disease

et al. 2020

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Humans have always been in contact with natural airborne particles from many sources including biologic particulate matter (PM) which can exhibit allergenic properties. With industrialization, anthropogenic and combustion-derived particles have become a major fraction. Currently, an ever-growing number of diverse and innovative materials containing engineered nanoparticles (NPs) are being developed with great expectations in technology and medicine. Nanomaterials have entered everyday products including cosmetics, textiles, electronics, sports equipment, as well as food, and food packaging. As part of natural evolution humans have adapted to the exposure to particulate matter, aiming to protect the individual's integrity and health. At the respiratory barrier, complications can arise, when allergic sensitization and pulmonary diseases occur in response to particle exposure. Particulate matter in the form of plant pollen, dust mites feces, animal dander, but also aerosols arising from industrial processes in occupational settings including diverse mixtures thereof can exert such effects. This review article gives an overview of the allergic immune response and addresses specifically the mechanisms of particulates in the context of allergic sensitization, effector function and therapy. In regard of the first theme (i), an overview on exposure to particulates and the functionalities of the relevant immune cells involved in allergic sensitization as well as their interactions in innate and adaptive responses are described. As relevant for human disease, we aim to outline (ii) the potential effector mechanisms that lead to the aggravation of an ongoing immune deviation (such as asthma, chronic obstructive pulmonary disease, etc.) by inhaled particulates, including NPs. Even though adverse effects can be exerted by (nano)particles, leading to allergic sensitization, and the exacerbation of allergic symptoms, promising potential has been shown for their use in (iii) therapeutic approaches of allergic disease, for example as adjuvants. Hence, allergen-specific immunotherapy (AIT) is introduced and the role of adjuvants such as alum as well as the current understanding of their mechanisms of action is reviewed. Finally, future prospects of nanomedicines in allergy treatment are described, which involve modern platform technologies combining immunomodulatory effects at several (immuno-)functional levels.

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Section: The Role Of Particulates In Allergic Sensitization—what Are mentioning

confidence: 99%

Mechanisms of Particles in Sensitization, Effector Function and Therapy of Allergic Disease

et al. 2020

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“…Our results showed one IgE-binding protein spot which corresponded to UTP-Glucose-1phosphate uridylyltransferases, as similarly reported in fruit latex 29 and short ragweed. 30 Beta xylosidase/alpha-L-arabinofuranosidase was another enzyme we identified in red oak pollen. This protein has been reported as an allergen in Aspergillus niger.…”

Section: Discussionmentioning

confidence: 94%

Proteomic identification of allergenic proteins in red oak (Quercus rubra) pollen

Huerta‐Ocampo

Valenzuela-Corral

Robles–Burgueño

et al. 2020

World Allergy Organization Journal

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Background: Red oak pollen is an important cause of allergic respiratory disease and it is widely distributed in North America and central Europe. To date, however, red oak pollen allergens have not been identified. Here, we describe the allergenic protein profile from red oak pollen. Methods: Total proteins were extracted from red oak pollen using a modified phenolic extraction method, and, subsequently, proteins were separated by two-dimensional gel electrophoresis (2DE) for both total protein stain (Coomassie Blue) and immunoblotting. A pool of 8 sera from red oak sensitive patients was used to analyze blotted proteins. Protein spots were analyzed by Mass Spectrometry. Results: Electrophoretic pattern of total soluble proteins showed higher intensity bands in the regions of 26-40 and 47-52 kDa. Two dimensional immunoblots using pool sera from patients revealed four allergenic proteins spots with molecular masses in the range from 50 to 55 kDa. Mass spectrometry analysis identified 8 proteins including Enolase 1 and Enolase 1 chloroplastic, Xylose isomerase (X1 isoform), mitochondrial Aldehyde dehydrogenase, UTP-Glusose-1phosphate uridylyltransferase, Betaxylosidase/alpha-L-arabinofuranosidase and alpha-and beta subunits of ATP synthase. Conclusions: This study has identified for first time 8 IgE binding proteins from red oak pollen. These findings will pave the way towards the development of new diagnostic and therapeutic modalities for red oak allergy.

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“…167 These are generated when ragweed is hydrated or exposed to a thunder storm. 167 These are generated when ragweed is hydrated or exposed to a thunder storm.…”

Section: Pollensmentioning

confidence: 99%

“…Smiljanic and colleagues have looked at the allergome of the ragweed subpollen particles which can be inspired into the lower airway. 167 These are generated when ragweed is hydrated or exposed to a thunder storm. The subpollen particles contained large amounts of the minor Amb a 4 and major Amb a 1 allergens.…”

Section: Pollensmentioning

confidence: 99%

Developments in the field of allergy in 2017 through the eyes of Clinical and Experimental Allergy

Roberts

Almqvist

Boyle

et al. 2018

Clin Experimental Allergy

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Subpollen particles are rich carriers of major short ragweed allergens and NADH dehydrogenases: quantitative proteomic and allergomic study

Abstract: We demonstrated that the complete major (Amb a 1 and 11) and almost all minor (Amb a 3, 4, 5, 6, 8 and 9) short ragweed pollen allergen repertoire as well as NADH oxidases are present in SPP, highlighting an important role for SPP in allergic sensitization to short ragweed.

Cited by 26 publications

References 43 publications

Mechanisms of Particles in Sensitization, Effector Function and Therapy of Allergic Disease

Mechanisms of Particles in Sensitization, Effector Function and Therapy of Allergic Disease

Proteomic identification of allergenic proteins in red oak (Quercus rubra) pollen

Developments in the field of allergy in 2017 through the eyes of Clinical and Experimental Allergy

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