Subpopulations of fetal‐like gingival fibroblasts: characterisation and potential significance for wound healing and the progression of periodontal disease

Schor, SL; Ellis, Ian R.; Irwin, Christopher; Banyard, J.; Seneviratne, K; Dolman, Clarisse L.; Gilbert, AD; Dm, Chisholm

doi:10.1111/j.1601-0825.1996.tb00217.x

Cited by 90 publications

(66 citation statements)

References 98 publications

(38 reference statements)

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“…The rate of healing is faster than that in the skin or other connective tissues and seems to be affected negligibly by age and gender [24,25]. Human oral mucosa-derived fibroblasts behave in some respects similarly to fetal-derived fibroblasts [26]. Approximately 44% of rodent oral mucosa-derived cells cycle in vivo and in vitro and on explantation additional $39% start cycling in vitro [27].…”

Section: Introductionmentioning

confidence: 99%

The Lamina Propria of Adult Human Oral Mucosa Harbors a Novel Stem Cell Population

et al. 2010

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The highly regenerative capacity of the human adult oral mucosa suggests the existence of a robust stem cell (SC) population in its lamina propria (OMLP). The purpose of this study was to characterize the availability, growth, immunophenotype, and potency of this presumable SC population. Cells positive for the embryonic stem cell transcription factors Oct4 and Sox2 and for p75 formed distinct cord-like structure in the OMLP. Regardless of donor age, trillions of cells, termed human oral mucosa stem cells (hOMSC), 95% of which express mesenchymal stromal cell markers, were simply, and reproducibly produced from a biopsy of 3-4 3 2 3 1 mm 3 . A total of 40-60% of these cells was positive for Oct4, Sox2, and Nanog and 60-80% expressed constitutively neural and neural crest SC markers. hOMSC differentiated in culture into mesodermal (osteoblastic, chondroblastic, and adipocytic), definitive endoderm and ectodermal (neuronal) lineages. Unexpectedly, hOMSC treated with dexamethasone formed tumors consisting of two germ layer-derived tissues when transplanted in severe combined immune deficiency mice. The tumors consisted of tissues produced by neural crest cells during embryogenesis-cartilage, bone, fat, striated muscle, and neural tissue. These results show that the adult OMLP harbors a primitive SC population with a distinct primitive neural-crest like phenotype and identifies the in vivo localization of putative ancestors for this population. This is the first report on ectodermal-and mesodermal-derived mixed tumors formation by a SC population derived from a nonmalignant somatic adult human tissue. STEM CELLS 2010;28:984-995 Disclosure of potential conflicts of interest is found at the end of this article.

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Section: Introductionmentioning

confidence: 99%

The Lamina Propria of Adult Human Oral Mucosa Harbors a Novel Stem Cell Population

et al. 2010

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“…Although gingiva is submitted to continuous and various injuries (mechanical, chemical, thermal, and infectious), it undergoes embryo-like repair (ie, without scar and fibrosis). 12,13 We recently showed in vitro and ex vivo that gingival fibroblasts (GF) dramatically reduced the aortic activity of MMP-9 related to increased TIMP-1 secretion, resulting in elastin preservation from degradation. 14 To further evaluate the concept of transfer of repair efficiency from 1 organ (gingiva) to compensate for the deficiency of another (artery), we aimed to evaluate in vivo the feasibility and efficacy of GF in established rabbit carotid aneurisms with a particular attention to the persistence and viability of transplanted GF in the arterial wall.…”

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confidence: 99%

Endoluminal Gingival Fibroblast Transfer Reduces the Size of Rabbit Carotid Aneurisms via Elastin Repair

Durand

Fournier

Couty

et al. 2012

ATVB

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Objective-Matrix metalloproteinase-9 is considered to play a pivotal role in aneurismal formation. We showed that gingival fibroblasts (GF) in vitro reduced matrix metalloproteinase-9 activity via increased secretion of tissue inhibitor of metalloproteinase 1. We aimed to evaluate in vivo the efficacy of GF transplantation to reduce aneurism development in a rabbit model. Methods and Results-Seventy rabbit carotid aneurisms were induced by elastase infusion. Four weeks later, GF, dermal fibroblast, or culture medium (DMEM) were infused into established aneurisms. Viable GF were abundantly detected in the transplanted arteries 3 months after seeding. GF engraftment resulted in a significant reduction of carotid aneurisms (decrease of 23.3% [P<0.001] and 17.6% [P=0.01] of vessel diameter in GF-treated arteries, 1 and 3 months after cell therapy, respectively), whereas vessel diameter of control DMEM and dermal fibroblast-treated arteries increased. GF inhibited matrix metalloproteinase-9 activity by tissue inhibitor of metalloproteinase 1 overexpression and matrix metalloproteinase-9/tissue inhibitor of metalloproteinase 1 complex formation, induced elastin repair, and increased elastin density in the media compared with DMEM-treated arteries (

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“…The multitude of responses developed by GFs probably refers to their capacity of differentiation into multiple cell types. 11 Indeed, GFs are now considered as progenitor cells 15,16 thanks to their high healing potentialities, and therefore their plasticity could be evaluated in other organs. Our study aimed to evaluate the ability of GFs to adequately respond to elastolysis.…”

mentioning

confidence: 99%

Preservation of Rabbit Aorta Elastin From Degradation by Gingival Fibroblasts in an Ex Vivo Model

Gogly

Naveau

Fournier

et al. 2007

ATVB

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Objective-Embryo-like gingival healing properties are attributed to the gingival fibroblast (GF) and could be used as a model for other types of healing dysfunctions. Abdominal aortic aneurysm (AAA) formation is associated with elastin degradation and increase in matrix metalloproteinase (MMP)-9 activity. We aimed to validate the concept of using GF healing properties in arteries. Methods and Results-We evaluated MMP-9 and its tissue inhibitor (TIMP-1) in rabbit aortic rings cultured in collagen gels with or without GFs and observed throughout 21 days. We also performed cocultures of human smooth muscle cells (hSMCs) with either gingival, dermal, or adventitial fibroblasts, and alone (control). In control arteries, elastic fibers became spontaneously sparse. In presence of GFs, elastic fibers were preserved. There was a dramatically reduced protein level of MMP-9 in coculture of aorta and GFs, in contrast with control aorta. MMP-9 expression was unaffected by GFs. MMP-9 inhibition was related to increased TIMP-1 secretion, TIMP-1 forming a complex with MMP-9. Cell cocultures of hSMC with GFs showed similar results. Dermal and adventitial fibroblasts did not affect MMP-9. Conclusions-Elastic fiber degradation was specifically preserved by GFs via reduction of MMP-9 protein level by increasing TIMP-1 synthesis. Vascular transfer of gingival fibroblasts could be a promising approach to treat AAA.

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Subpopulations of fetal‐like gingival fibroblasts: characterisation and potential significance for wound healing and the progression of periodontal disease

Cited by 90 publications

References 98 publications

The Lamina Propria of Adult Human Oral Mucosa Harbors a Novel Stem Cell Population

The Lamina Propria of Adult Human Oral Mucosa Harbors a Novel Stem Cell Population

Endoluminal Gingival Fibroblast Transfer Reduces the Size of Rabbit Carotid Aneurisms via Elastin Repair

Preservation of Rabbit Aorta Elastin From Degradation by Gingival Fibroblasts in an Ex Vivo Model

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