Subrepeats in 5s rDNAs as a molecular marker in Acer platanoides L. populations

Панчук, І. І.; Kasianchuk, R. M.; Волков, Р. А.

doi:10.7124/feeo.v25.1143

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…When compared with other European broadleaved forest tree species, A. platanoides has been little studied using modern genetic markers (Panchuk et al 2019). At the same time, genetic diversity and population structure of Q. robur is well investigated in Western and Central Europe (Petit et al 2002) and, in the last years, in the Russian part of the distribution range, including the Southern Urals.…”

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Norway maple (Acer platanoides) and pedunculate oak (Quercus robur) demonstrate different patterns of genetic variation within and among populations on the eastern border of distribution ranges

Akhmetov¹,

Ianbaev²,

Boronnikova³

et al. 2021

J. For. Sci.

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Norway maple (Acer platanoides L.) is a key species of broadleaved forests whose population genetics is poorly studied using modern genetic tools. We used ISSR analysis to explore genetic diversity and differentiation among 10 Russian populations on the eastern margin of the species range of distribution, and to compare the revealed patterns with the results of our population genetic studies of pedunculate oak (Quercus robur L.). In the first set comparatively high heterozygosity and allelic diversity were found (expected heterozygosity HE = 0.160 ± 0.033, number of alleles na = 1.440 ± 0.080, effective number of alleles ne = 1.271 ± 0.062) in comparison with strongly fragmented and geographically isolated small maple stands of the second set (HE = 0.083 ± 0.011, na = 1.281 ± 0.031, ne = 1.136 ± 0.019). A relatively high genetic differentiation among populations was detected (the proportion of the inter-population component of total genetic variation, GST = 0.558 ± 0.038). In the Cis-Urals, local groups of populations that are confined to the northern, middle and southern parts of the Urals were identified. On the contrary, the current significant fragmentation of the pedunculate oak distribution area in the same study area did not lead to any noticeable genetic differentiation among the majority of populations, the values of the population genetic diversity were very similar in different parts of the Southern Urals.

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Norway maple (Acer platanoides) and pedunculate oak (Quercus robur) demonstrate different patterns of genetic variation within and among populations on the eastern border of distribution ranges

Akhmetov¹,

Ianbaev²,

Boronnikova³

et al. 2021

J. For. Sci.

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“…Завдяки цим властивостям 5S рДНК є зручним інструментом для вивчення закономірностей молекулярної еволюції та з'ясування генетичних дистанцій між близько спорідненими таксонами. Раніше ефективність використання цієї ділянки у таксономічних дослідженнях було успішно представлено для родів Solanum [11], Acer [12,13], Lathyrus [14], Vinga [15], триб Poeae [16][17][18], Helieae [19] та інших груп Покритонасінних рослин. 5S рДНК була також використана у таксономічних дослідженнях роду Quercus, проте, переважно для західноєвразійських представників секцій Quercus, Cerris та Illex [20,21].…”

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Organization of the 5s rDNA intergenic spacer of Quercus rubra L. and its relationship to the ukrainian Quercus species

Tynkevich¹,

Bushyla²,

Волков³

2020

Fakt. eksp. evol. org.

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Aim. Quercus rubra L. is a representative of the North American section Lobatae, which has naturalized in Ukraine and becomes an invasive species in some places. Accordingly, elucidation of its relationship with the Ukrainian species of section Quercus is relevant to assess the possibility of interspecific hybridization. The 5S rDNA intergenic spacer (IGS) represents a convenient molecular marker to address this question. Methods. DNA isolation, PCR amplification, cloning and sequencing. Results. The IGS of Q. rubra was cloned and sequenced. It was shown that after the divergence of the Lobatae and Quercus sections, the IGS sequences evolved mainly by accumulation of nucleotide substitutions. A high level of IGS similarity was found for species within the Labatae and Quercus sections, while the genetic distances between the representatives of these two sections appeared significantly higher. Conclusions. The high level of nucleotide substitutions indicates a considerable genetic distance between the Lobatae and Quercus sections and calls into question the possibility of spontaneous hybridization between Q. rubra and Ukrainian native species of the section Quercus. At the same time, the close genetic relationship between other North American species, Q. lobata and Ukrainian "white oaks" indicate that introduction of representatives of this group may create conditions for their spontaneous crossing with the native species, which could be undesirable for the conservation of Ukraine's natural forest-steppe communities. Sequencing of the 5S rDNA IGS can be used for molecular genotyping of the Quercus species and for identification of interspecific hybrids. Keywords: 5S rDNA, molecular evolution, Quercus, Lobatae.

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Organization of 5S rDNA of field maple (Acer campestre L.)

Ishchenko

Панчук

Волков

2019

Biolohichni systemy

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In recent decades, molecular methods have been widely used to study plant speciation and taxonomy. The 5S rDNA, which belongs to the class of repeated sequences present in the genomes of all eukaryotes, represents a convenient model for studying the patterns of molecular evolution in plants. Each of 5S rDNA repeated units consist of a coding region and an intergenic spacer (IGS). The coding sequence is highly conserved in evolution, whereas the IGS can differ between related species and even between populations of the same species. The molecular organization of the 5S rDNA in representatives of genus Acer is still poorly explored. Accordingly, the aim of the study was to investigate the organization and variability of the 5S rDNA of the European species Acer campestre. Analysis of the obtained sequences showed, that the 5S rDNA clones of A. campestre are identical. The level of IGS sequence similarity between A. сampestre and A. platanoides amounts to 96.1%, whereas the similarity between these species and A. pseudoplatanus is lower, namely – 81,9–82,4%. Therefore, A. campestre and A. platanoides can be considered as closely related species. Only one class of 5S rDNA repeats is present in the genome of A. сampestre. The potential external elements of the RNA polymerase III promoter localized in the IGS differ from those described early for representatives of other families of angiosperms.

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Subrepeats in 5s rDNAs as a molecular marker in Acer platanoides L. populations

Cited by 4 publications

References 23 publications

Norway maple (Acer platanoides) and pedunculate oak (Quercus robur) demonstrate different patterns of genetic variation within and among populations on the eastern border of distribution ranges

Norway maple (Acer platanoides) and pedunculate oak (Quercus robur) demonstrate different patterns of genetic variation within and among populations on the eastern border of distribution ranges

Organization of the 5s rDNA intergenic spacer of Quercus rubra L. and its relationship to the ukrainian Quercus species

Organization of 5S rDNA of field maple (Acer campestre L.)

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