Subsets of tonsillar lymphocytes and activated cells in each subset analysed by Two-color flow cytometry

Sugiyama, Masao; Yamane, Hideo; Konishi, Kazuo; Uekawa, Manabu; Tamura, Takayuki; Nakai, Yousuke

doi:10.3109/00016488709107338

Cited by 11 publications

(2 citation statements)

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“…T lymphocytes purified from human tonsils are mainly CD4 cells (Sugiyama et al, 1976(Sugiyama et al, , 1984(Sugiyama et al, , 1987Plum et al, 1987), CD8 T cells being proportionally less numerous in tonsils than in the peripheral blood (Sugiyama et al, 1987). The prevalence of CD4 cells in tonsils suggests that tonsillar T lymphocytes are functionally oriented to helper activity (Sugiyama et al, 1987). Several immunohistochemical studies have revealed the existence of tonsillar T cells expressing the CD57 antigen characteristic of cells with NK activity (Okada et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Human Germinal Center CD4⁺ CD57⁺ T Cells ActDifferently On B Cells Than Do Classical T‐Helper Cells

Bouzahzah

Bosseloir

Heinen

et al. 1994

Journal of Immunology Research

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We have isolated two subtypes of helper T cells from human tonsils: CD4+ CD57+ cells, mostly located in the germinal center (GC), and CD4+ CD57- cells, distributed through the interfollicular areas but also present in the GC. In a functional study, we have compared the capacities of these T-cell subtypes to stimulate B cells in cocultures. In order to block T-cell proliferation while maintaining their activation level, we pretreated isolated T cells with mitomycin C prior to culture in the presence of B cells and added polyclonal activators such as PHA and Con A, combined or not with IL-2. Contrary to CD4+ CD57- cells, CD4+ CD57+ cells did not markedly enhance B-cell proliferation. Even when sIgD-B cells typical of germinal center cells were tested, the CD4 CD57 cells had no significant effect. This is in accordance with the location of these cells: They mainly occupy the light zones of the GC where few B cells divide. Even when added to preactivated, actively proliferating cells, CD4+ CD57+ cells failed to modulate B-cell multiplication. On the supernatants of B-cell-T-cell cocultures, we examined by the ELISA technique the effect of T cells on Ig synthesis. Contrary to CD57- T cells, whose effect was strong, CD57+ T cells weakly stimulated Ig synthesis. More IgM than IgG was generally found. Because CD57 antigen is a typical marker of natural killer cells, we tested the cytolytic activity of tonsillar CD4+ CD57+ cells on K562 target cells. Unlike NK cells, neither CD4+CD57+ nor CD4+ CD57- cells exhibit any cytotoxicity. Thus, germinal center CD4+ CD57+ cells are not cytolytic and do not strongly stimulate either B-cell proliferation or Ig secretion. CD4+ CD57- cells, however, enhance B-cell proliferation and differentiation, thus acting like the classical helper cells of the T-dependent areas.

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Section: Introductionmentioning

confidence: 99%

Human Germinal Center CD4⁺ CD57⁺ T Cells ActDifferently On B Cells Than Do Classical T‐Helper Cells

Bouzahzah

Bosseloir

Heinen

et al. 1994

Journal of Immunology Research

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“…Tonsils were classified on the basis of clinical and pathohistological diagnoses in HT and RT according to Sugiyama et al [5]. After excision, the specimens for enzyme detection were immediately frozen.…”

Section: Tissue Specimensmentioning

confidence: 99%

Localisation of Ecto-5{LC2}’- Nucleotidase and Divalent Cation-Activated Ecto-ATPase in Chronic Tonsillitis

Avramović¹,

Vlahović²,

Savić³

et al. 1998

ORL

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The histochemical localisation of two ecto-enzymes, 5′-nucleotidase (5′-NT) and Mg²⁺-ATPase, was investigated in hyperplastic and recurrent tonsillitis. Detection of enzymes was performed on frozen sections using the classical lead nitrate method. Activity of 5′-NT was demonstrated particularly in the cells of lymphoid follicles and in the basal layer of the surface tonsillar epithelium. There was no difference in localisation of 5′-NT between hyperplastic and recurrent tonsillitis, whereas a stronger reaction in follicular mantle zones was observed in recurrent tonsillitis compared to hyperplastic tonsillitis. Mg²⁺-ATPase activity was mainly associated with the cells lining the tonsillar crypt, with the interfollicular areas and blood vessels. In recurrent tonsillitis only half of the studied follicular germinal centres expressed Mg²⁺-ATPase activity, compared to hyperplastic tonsillitis. The similar localisation of 5′-NT and ecto-ATPase in both types of chronic tonsillitis suggests that in inflamed tonsils expression of investigated enzymes probably does not depend on the type of chronic tonsillitis.

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Immunohistological Expression of Interleukin 16 in Human Tonsils

Krämer¹,

Mack²,

Rasp³

2001

Arch Otolaryngol Head Neck Surg

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Interleukin 16 was mainly expressed in a typical CD4-like pattern in human tonsils. Our data strongly suggest that CD4(+) lymphocytes constitute the major cellular source for IL-16. We hypothesize that the double-immunostained CD4-ir and IL-16-ir cells represent activated T cells. Because CD22(+) B cells at the outer rim of the mantle zone expressed IL-16 as well, we conclude that this area might constitute the locus of IL-16-mediated B-cell differentiation.

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Subsets of tonsillar lymphocytes and activated cells in each subset analysed by Two-color flow cytometry

Cited by 11 publications

References 13 publications

Human Germinal Center CD4⁺ CD57⁺ T Cells ActDifferently On B Cells Than Do Classical T‐Helper Cells

Human Germinal Center CD4⁺ CD57⁺ T Cells ActDifferently On B Cells Than Do Classical T‐Helper Cells

Localisation of Ecto-5{LC2}’- Nucleotidase and Divalent Cation-Activated Ecto-ATPase in Chronic Tonsillitis

Immunohistological Expression of Interleukin 16 in Human Tonsils

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Subsets of tonsillar lymphocytes and activated cells in each subset analysed by Two-color flow cytometry

Cited by 11 publications

References 13 publications

Human Germinal Center CD4+ CD57+ T Cells ActDifferently On B Cells Than Do Classical T‐Helper Cells

Human Germinal Center CD4+ CD57+ T Cells ActDifferently On B Cells Than Do Classical T‐Helper Cells

Localisation of Ecto-5{LC2}’- Nucleotidase and Divalent Cation-Activated Ecto-ATPase in Chronic Tonsillitis

Immunohistological Expression of Interleukin 16 in Human Tonsils

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Human Germinal Center CD4⁺ CD57⁺ T Cells ActDifferently On B Cells Than Do Classical T‐Helper Cells

Human Germinal Center CD4⁺ CD57⁺ T Cells ActDifferently On B Cells Than Do Classical T‐Helper Cells