Subspecies limits and hidden <i>Wolbachia</i> diversity in <i>Actinote pellenea</i> butterflies

Silva-Brandão, Karina Lucas; Cirino, Mariana; Magaldi, Luiza de Moraes; Gueratto, Patrícia Eyng; Mattos, Ricardo Gabriel; Freitas, André Victor Lucci

doi:10.1080/14772000.2021.1965669

Cited by 6 publications

(4 citation statements)

References 61 publications

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“…By detecting Spiroplasma in adult Morpho males sampled in the wild, our study suggests that the presence of Spiroplasma does not prevent the development of males in Morpho butterflies, in sharp discrepancy with the male-killing effects reported in the butterfly Danaus chrysippus (Jiggins et al 2000), but similar to the results obtained with Wolbachia in Neotropical Acraeini (Nymphalidae) (Silva-Brandão et al 2021). More specifically, the toxin gene Spaid found in Spiroplasma poulsonii and documented to trigger male killing in Drosophila (Harumoto and Lemaitre 2018) does not have the male-killing (MK) effect in Morpho .…”

Section: Discussioncontrasting

confidence: 55%

Wild communities ofMorphobutterflies revealSpiroplasmaendosymbiont with inflated genome size and peculiar evolution

Filée,

López-Villavicencio,

Debat

et al. 2024

Preprint

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The evolution of endosymbiont genomes is likely influenced by the ecological interactions with their hosts. Here, we studied the evolution ofSpiroplasmagenomes detected withinMorphobutterflies sampled in the wild.Spiroplasmawas detected in 4 out of the 11Morphospecies studied and displayed a 3 times larger genome size as compared toSpiroplasmagenomes documented in other hosts. This inflation in genome size is caused by massive and recent expansion of various mobile genetic elements and by the acquisition of new genes stemming from prophages. In particular, we documented the peculiar evolution of the toxin genes in plasmids that may enhance host resistance to parasites. Phylogenetic comparisons withSpiroplasmaextracted from other host point at a unique origin ofSpiroplasmainMorpho, and strong divergence fromSpiroplasmafound in other Lepidoptera. Resequencing data obtained for multiple populations of the two sister-speciesM. helenorandM. achillesliving in sympatry over the majority of their distribution revealed a opposite prevalence (97% inM. achillesand 3% inM. helenor), suggesting contrasted ecological interactions with these two host-species. Reconciliation analysis of the phylogenetic relationships ofMorphomitochondrial genomes andSpiroplasmagenomes was then consistent with a predominant vertical transfer of the endosymbiont. Altogether, our results suggest a key role of ecological interactions with the host in the evolution of endosymbiont genomes and point at a putative interaction ofSpiroplasmawith reproductive isolation between sympatric species of butterflies.

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Section: Discussioncontrasting

confidence: 55%

Wild communities ofMorphobutterflies revealSpiroplasmaendosymbiont with inflated genome size and peculiar evolution

Filée,

López-Villavicencio,

Debat

et al. 2024

Preprint

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“…Furthermore, there is no genetic structure across locations based on COI, and all species delimitation methods evaluated failed to tell the two species apart. Some hypotheses have already been raised to explain this lack of information at the COI level, such as a lower diversification rate also found for other Acraeini species, intrinsically different rates of molecular evolution, or selective sweeps by endosymbionts, as already demonstrated for other Acraeini (Jiggins, 2003; Silva‐Brandão et al., 2021), and in fact all mechanisms can contribute to explaining the present pattern for this mitochondrial marker.…”

Section: Discussionmentioning

confidence: 61%

“…Indeed, while A. mantiqueira and A. alalia can be distinguished by a series of distinct morphological characteristics of adults, immature stages, and geographic distribution (Freitas et al, 2018), the COI barcode lacks sufficient interspecific differences to distinguish them. Therefore, based on barcodes, the genetic distance between individuals of these species is virtually zero, that is, there is no "barcode gap" (sensu Hebert et al, 2004) (Jiggins, 2003;Silva-Brandão et al, 2021),…”

Section: Taxonomic Delimitation Of Species Using Different Molecular ...mentioning

confidence: 99%

Montane diversification as a mechanism of speciation in neotropical butterflies

de Moraes Magaldi,

Gueratto,

Ortega‐Abboud

et al. 2024

Ecology and Evolution

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The mountains in the Atlantic Forest domain are environments that harbor a high biodiversity, including species adapted to colder climates that were probably influenced by the climatic variations of the Pleistocene. To understand the phylogeographic pattern and assess the taxonomic boundaries between two sister montane species, a genomic study of the butterflies Actinote mantiqueira and A. alalia (Nymphalidae: Acraeini) was conducted. Analyses based on partial sequences of the mitochondrial gene COI (barcode region) failed to recover any phylogenetic or genetic structure discriminating the two species or sampling localities. However, single nucleotide polymorphisms gathered using Genotyping‐by‐Sequencing provided a strong isolation pattern in all analyses (genetic distance, phylogenetic hypothesis, clustering analyses, and FST statistics) which is consistent with morphology, separating all individuals of A. alalia from all populations of A. mantiqueira. The three sampled mountain ranges where A. mantiqueira populations occur—Serra do Mar, Serra da Mantiqueira, and Poços de Caldas Plateau—were identified as three isolated clusters. Paleoclimate simulations indicate that both species' distributions changed according to climatic oscillations in the Pleistocene period, with the two species potentially occurring in areas of lower altitude during glacial periods when compared to the interglacial periods (as the present). Besides, a potential path between their distribution through the Serra do Mar Mountain range was inferred. Therefore, the Pleistocene climatic fluctuation had a significant impact on the speciation process between A. alalia and A. mantiqueira, which was brought on by isolation at different mountain summits during interglacial periods, as shown by the modeled historical distribution and the observed genetic structure.

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“…Furthermore, we analysed the Wolbachia recombination and transmission patterns across Tagiadini species and revealed the potential effects of Wolbachia on variation and evolution of mtDNA. While Wolbachia has been investigated in many lepidopteran insects with a particular focus on infection status, sequence information, recombination or transmission patterns (Andersen et al., 2019 ; Arif et al., 2021 ; Bereczki et al., 2015 ; Dincă et al., 2019 ; Gaunet et al., 2019 ; Gislaine et al., 2018 ; Hinojosa et al., 2019 ; Jiang et al., 2014 , 2016 , 2018 ; Silva‐Brandão et al., 2021 ; Śliwińska et al., 2019 ; Yudina et al., 2016 ; Zhang et al., 2021 ), there are few studies that focus on the inter‐specific level across a large area. This has led us to complete a comprehensive survey of Wolbachia across the Tagiadini.…”

Section: Introductionmentioning

confidence: 99%

Wolbachia infection status and molecular diversity in the species of tribe Tagiadini Mabille, 1878 (Lepidoptera: Hesperiidae) collected in China

Wei,

Zhu,

Hoffmann

et al. 2024

Ecology and Evolution

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Wolbachia, one of the most ubiquitous heritable symbionts in lepidopteran insects, can cause mitochondrial introgression in related host species. We recently found mito‐nuclear discordance in the Lepidopteran tribe Tagiadini Mabille 1878 from which Wolbachia has not been reported. In this study, we found that 13 of the 46 species of Tagiadini species tested were positive for Wolbachia. Overall, 14% (15/110) of Tagiadini specimens were infected with Wolbachia and nine new STs were found from 15 isolates. A co‐phylogenetic comparison, divergence time estimation and Wolbachia recombination analysis revealed that mito‐nuclear discordance in Tagiadini species is not mediated by Wolbachia, but Wolbachia acquisition in Tagiadini appears to have occurred mainly through horizontal transmission rather than codivergence.

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Subspecies limits and hidden Wolbachia diversity in Actinote pellenea butterflies

Cited by 6 publications

References 61 publications

Wild communities ofMorphobutterflies revealSpiroplasmaendosymbiont with inflated genome size and peculiar evolution

Wild communities ofMorphobutterflies revealSpiroplasmaendosymbiont with inflated genome size and peculiar evolution

Montane diversification as a mechanism of speciation in neotropical butterflies

Wolbachia infection status and molecular diversity in the species of tribe Tagiadini Mabille, 1878 (Lepidoptera: Hesperiidae) collected in China

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