Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays

Abstract

A new technique for coating microtitre plates with molecularly imprinted polymers (MIP), specific for low-molecular weight analytes (epinephrine, atrazine) and proteins is presented. Oxidative polymerization was performed in the presence of template; monomers: 3-aminophenylboronic acid (APBA), 3-thiopheneboronic acid (TBA) and aniline were polymerized in water and the polymers were grafted onto the polystyrene surface of the microplates. It was found that this process results in the creation of synthetic materials with antibody-like binding properties. It was shown that the MIP-coated microplates are particularly useful for assay development. The high stability of the polymers and good reproducibility of the measurements make MIP coating an attractive alternative to conventional antibodies or receptors used in enzyme linked immunosorbent assay (ELISA).

Does size matter? Study of performance of pseudo-ELISAs based on molecularly imprinted polymer nanoparticles prepared for analytes of different sizes

“…One of the major drawbacks to work with MIPs in ELISA is the absence of a reproducible and straightforward method for coating microplate wells with the material. In the literature, there are several papers describing the applications of MIPs in ELISA, [8][9][10][11][12][13][14][15] but only a few demonstrate direct application of MIPs in the assays for quantitative detection of the target analytes. [8][9]16 In one example, the surface of the microplate wells was modified with a homopolymer of 3-aminophenylboronic acid, which was imprinted with ephedrine 15 .…”

Molecularly imprinted solid-phase extraction combined with high performance liquid chromatography for analysis of phenolic compounds from environmental water samples

“…These materials have been employed in fields where a certain degree of selectivity is required such as chromatography [11,[16][17][18], sensors [19,20], immunoassays [21,22] and catalysts [23,24]. The most significant advantages of MIPs, as compared to biological receptors, such as antibodies, enzymes, nucleic acids, or cells, include their mechanical and chemical stability, high selectivity, low cost of preparation, and wide range of operating conditions [25,26].…”

Synthesis and binding site characteristics of 2,4,6-trichlorophenol-imprinted polymers

“…MIPs offer some advantages, for example, they are stable to a wide range of pressures, organic solvents, and pHs. These properties make MIPs attractive for many analytical applications as stationary phases for chromatographic separations [3,4], for molecular and ionic separations by selective sorption [5,6], as recognition entities in sensors [7,8], in immunoassays [9,10], and as catalysts [11,12].…”