Substoichiometric shifting in the plant mitochondrial genome is influenced by a gene homologous to MutS

Abdelnoor, R. V.; Yule, Ryan; Elo, Annakaisa; Christensen, Alan C.; Meyer-Gauen, Gilbert; Mackenzie, Sally A.

doi:10.1073/pnas.1037651100

Cited by 233 publications

(258 citation statements)

References 58 publications

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“…Similarly, but unexpectedly, the signal detected in var2-6 (a nonsense mutation by T-DNA insertion) by Anti-VAR1 was as low as that in var1 alleles (Figure 7A). This finding could not be attributed to the variegation itself, because another variegated mutant, chm1-2, the responsible gene of which is unrelated to FtsH (Abdelnoor et al, 2003), did accumulate the VAR1 signal just like Col. Therefore, we suspected that the lack of VAR2 was accompanied by a loss of VAR1.…”

Section: The Accumulation Of Var1 and Var2 Is Regulated Coordinately mentioning

confidence: 88%

Coordinated Regulation and Complex Formation of YELLOW VARIEGATED1 and YELLOW VARIEGATED2, Chloroplastic FtsH Metalloproteases Involved in the Repair Cycle of Photosystem II in Arabidopsis Thylakoid Membranes

et al. 2003

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Arabidopsis YELLOW VARIEGATED1 ( VAR1 ) and VAR2 are separate loci that encode similar chloroplast FtsH proteases. To date, FtsH is the best-characterized protease in thylakoid membranes involved in the turnover of photosynthetic protein complexes. It comprises a protein family that is encoded by 12 different nuclear genes in Arabidopsis. We show here that nine FtsH proteins are located in the chloroplasts. Mutations in either VAR1 or VAR2 cause typical leaf variegation and sensitivity to photoinhibition. By contrast, none of these phenotypes was observed in T-DNA insertion mutants in other ftsH genes ( ftsh1 , ftsh6 , and ftsh8 ) closely related to VAR1 and VAR2 . This finding suggests that VAR1 and VAR2 play a predominant role in the photosystem II repair cycle in thylakoid membranes. By generating VAR1-and VAR2-specific antibodies, we found that loss of either VAR1 or VAR2 results in the decreased accumulation of the other. Thus, the genetic nonredundancy between VAR1 and VAR2 could be attributed to their coordinated regulation at the protein level. These observations led us to examine whether VAR1 and VAR2 form a complex. Sucrose density gradient and gel filtration analyses revealed a complex of ‫ف‬ 400 to 450 kD, probably representing a hexamer. Furthermore, VAR1 and VAR2 were shown to coprecipitate by immunoprecipitation using VAR1-and VAR2-specific antibodies. The majority of VAR1 appears to exist as heterocomplexes with VAR2, whereas VAR2 may be present as homocomplexes as well. Based on these results, we conclude that VAR1 and VAR2 are the major components of an FtsH complex involved in the repair of photodamaged proteins in thylakoid membranes.

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Section: The Accumulation Of Var1 and Var2 Is Regulated Coordinately mentioning

confidence: 88%

Coordinated Regulation and Complex Formation of YELLOW VARIEGATED1 and YELLOW VARIEGATED2, Chloroplastic FtsH Metalloproteases Involved in the Repair Cycle of Photosystem II in Arabidopsis Thylakoid Membranes

et al. 2003

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“…Cloning of CHM revealed that it encodes a gene homologous with Escherichia coli MutS, which is involved in mismatch repair and DNA recombination (Abdelnoor et al, 2003). The investigators have proposed that CHM may be required for recombination or replication and, therefore, that mutation could result in modulation of the copy number of particular molecules (Mackenzie, 2004).…”

Section: Fertility Restoration Through Mtdna Alterationmentioning

confidence: 99%

Interactions of Mitochondrial and Nuclear Genes That Affect Male Gametophyte Development

Hanson

Bentolila

2004

THE PLANT CELL ONLINE

754

623

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“…Under some conditions, however, the DNA-repair machinery is unable to cope with all DNA damage and non-conservative repairs occur (Abdelnoor et al, 2003;Cappadocia et al, 2010;Kwon et al, 2010;Maré chal et al, 2009;Shedge et al, 2007). Whirly proteins are negative regulators of a non-conservative repair pathway named microhomology-mediated break-induced replication (MMBIR; Maré chal et al, 2009;Cappadocia et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

A family portrait: structural comparison of the Whirly proteins fromArabidopsis thalianaandSolanum tuberosum

Cappadocia

Parent

Sygusch

et al. 2013

Acta Crystallogr F Struct Biol Cryst Commun

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PDB references: WHY1, 4koo; WHY2, 4kop; WHY3, 4koq DNA double-strand breaks are highly detrimental genomic lesions that routinely arise in genomes. To protect the integrity of their genetic information, all organisms have evolved specialized DNA-repair mechanisms. Whirly proteins modulate DNA repair in plant chloroplasts and mitochondria by binding single-stranded DNA in a non-sequence-specific manner. Although most of the results showing the involvement of the Whirly proteins in DNA repair have been obtained in Arabidopsis thaliana, only the crystal structures of the potato Whirly proteins WHY1 and WHY2 have been reported to date. The present report of the crystal structures of the three Whirly proteins from A. thaliana (WHY1, WHY2 and WHY3) reveals that these structurally similar proteins assemble into tetramers. Furthermore, structural alignment with a potato WHY2-DNA complex reveals that the residues in these proteins are properly oriented to bind single-stranded DNA in a non-sequence-specific manner.

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Substoichiometric shifting in the plant mitochondrial genome is influenced by a gene homologous to MutS

Cited by 233 publications

References 58 publications

Coordinated Regulation and Complex Formation of YELLOW VARIEGATED1 and YELLOW VARIEGATED2, Chloroplastic FtsH Metalloproteases Involved in the Repair Cycle of Photosystem II in Arabidopsis Thylakoid Membranes

Coordinated Regulation and Complex Formation of YELLOW VARIEGATED1 and YELLOW VARIEGATED2, Chloroplastic FtsH Metalloproteases Involved in the Repair Cycle of Photosystem II in Arabidopsis Thylakoid Membranes

Interactions of Mitochondrial and Nuclear Genes That Affect Male Gametophyte Development

A family portrait: structural comparison of the Whirly proteins fromArabidopsis thalianaandSolanum tuberosum

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