Substrate Affinity and Specificity of the<i>Sc</i>Sth1p Bromodomain Are Fine-Tuned for Versatile Histone Recognition

Blus, Bartlomiej J.; Hashimoto, Hideharu; Seo, Hyuk‐Soo; Królak, Aleksandra; Debler, Erik

doi:10.1101/559005

Cited by 1 publication

(2 citation statements)

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“…S3B; compare gcn5 heatmap with WT heatmap, S3C), indicating that Gcn5 is needed for recruiting or retaining high RSC occupancies over the ORFs of transcribed genes. Although acetylated H3K14 (acetylated by Gcn5) enhances the binding of the Sth1 BrD to nucleosomes in vitro and in vivo (40)(41)(42), sth1BrD showed minimal reductions in RSC occupancy compared to gcn5. The minor reductions in RSC occupancies in the sth1BrD mutant suggest that other RSC bromodomains might act redundantly to recruit RSC to transcribed ORFs.…”

Section: Hats Gcn5 and Esa1 Promote Rsc Recruitment To Transcribed Orfsmentioning

confidence: 98%

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The role of histone acetyltransferases Gcn5 and Esa1 in recruiting the RSC complex and maintaining nucleosome-depleted regions genome-wide in Saccharomyces cerevisiae

Biernat

Verma

Werick

et al. 2022

Preprint

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Chromatin remodelers are essential for the maintenance of chromatin structure and gene regulation. In this study, we examined the role of histone acetyltransferases (HATs) Gcn5 and Esa1 in regulating RSC and histone occupancies and their effects on transcription genome-wide. We identified contrasting roles of HATs in modulating RSC occupancies in promoters and ORFs. In HAT mutants, RSC accumulated in nucleosome depleted regions (NDRs) with fragile nucleosomes (FNs) more than those with stable -1 nucleosomes. Moreover, the accumulation was more significant in the Esa1 mutant than in the Gcn5 mutant. However, RSC NDR accumulation was not observed in cells lacking H3 or H4 tails. Furthermore, we observed marked increases in histone occupancies in NDRs in the HAT mutants genome-wide. Overall, these data suggest that FNs use hypoacetylated tails to recruit RSC to NDRs, and subsequent acetylation of the tails promote histone eviction. In contrast to the promoters, RSC occupancies were significantly reduced in transcribed ORFs in the HAT mutants. Additionally, the HAT mutants showed reduced TBP and Pol II binding at promoters. Thus, our data implicate HATs and RSC in maintaining NDRs, regulating chromatin structure, and promoting transcription.

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Section: Hats Gcn5 and Esa1 Promote Rsc Recruitment To Transcribed Orfsmentioning

confidence: 98%

“…RSC BrDs have been shown to bind acetylated lysines (38,39). For instance, the Sth1 bromodomain binds to acetylated H3K14 in vitro and in vivo (40)(41)(42). Since RSC contains many bromodomains, acetylation may direct RSC recruitment to promoters, including at FNs and in transcribed open reading frames (ORFs) (17,24,43).…”

Section: Introductionmentioning

confidence: 99%