1998
DOI: 10.1006/jmbi.1998.1890
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Substrate assistance in the mechanism of family 18 chitinases: theoretical studies of potential intermediates and inhibitors 1 1Edited by B. Honig

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Cited by 104 publications
(62 citation statements)
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“…3), the observed eŠect of the ChBDChiC deletion on the antifungal activity may not be ascribed solely to reduced chitinolytic activity, implying an additional antifungal role of ChBDChiC. 6 . We injected 10.0 mM (GlcNAc) 6 into 67.6 mM ChBD ChiC at 259 C. The reaction heats after subtraction of dilution heats wereˆtted to the theoretical curve (solid line) with the assumption of a single binding site.…”
Section: Interaction Of Chbd Chic and Chic With Various Chitinous Submentioning
confidence: 95%
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“…3), the observed eŠect of the ChBDChiC deletion on the antifungal activity may not be ascribed solely to reduced chitinolytic activity, implying an additional antifungal role of ChBDChiC. 6 . We injected 10.0 mM (GlcNAc) 6 into 67.6 mM ChBD ChiC at 259 C. The reaction heats after subtraction of dilution heats wereˆtted to the theoretical curve (solid line) with the assumption of a single binding site.…”
Section: Interaction Of Chbd Chic and Chic With Various Chitinous Submentioning
confidence: 95%
“…ITC experiments were done using an OMEGA calorimeter (MicroCal Inc., Amherst, Mass.). The reaction cell (1.4 ml volume) wasˆlled with 50 mM sodium phosphate buŠer (pH 6.0) containing 67.6 mM ChBDChiC, and 10 ml of 10.0 mM (GlcNAc) 6 in the same buŠer was injected 22 times into the reaction cell through a syringe every 6 min at 259 C. In a control experiment, the (GlcNAc)6 solution was also injected into the buŠer without ChBDChiC to obtain the heat on dilution. The dilution heats were subtracted from the observed reaction heats.…”
Section: Sds-polyacrylamide Gel Electrophoresis (Sds-page)mentioning
confidence: 99%
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“…To explain the mechanism by which hydrolysis occurs via a single glutamate, a ''substrate-assisted catalysis'' model has been proposed [2,9,10]. According to this model, the catalytic residue (associated glutamate) donates a proton to the oxygen atom of the glycosidic linkage.…”
Section: Introductionmentioning
confidence: 99%