Substrate topography determines the fate of chondrogenesis from human mesenchymal stem cells resulting in specific cartilage phenotype formation

Wu, Yingnan; Law, J. B. K.; He, Ai Yu; Low, Hong Yee; Hui, James; Lim, Chwee Teck; Yang, Zheng; Lee, Eng Hin

doi:10.1016/j.nano.2014.04.002

Cited by 109 publications

(99 citation statements)

References 44 publications

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“…Béduer et al [51] hMSC PDMS Micropatterned with striped groove morphology coated with collagen type I Neuronal Biehl et al [52] hESC PDMS Groove Neuronal Lu et al [53] hMSC PCL Nanopillar, nanohole and nanogrill Nanopillar and nanohole topography enhanced Wu et al [54] hNSC PUA Groove and Pillar MSC chondrogenesis and facilitated hyaline cartilage. Neuronal…”

Section: Neuronalmentioning

confidence: 99%

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Control of stem cell fate by engineering their micro and nanoenvironment

Griffin

Butler

Seifalian

et al. 2015

WJSC

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Stem cells are capable of long-term self-renewal and differentiation into specialised cell types, making them an ideal candidate for a cell source for regenerative medicine. The control of stem cell fate has become a major area of interest in the field of regenerative medicine and therapeutic intervention. Conventional methods of chemically inducing stem cells into specific lineages is being challenged by the advances in biomaterial technology, with evidence highlighting that material properties are capable of driving stem cell fate. Materials are being designed to mimic the clues stem cells receive in their in vivo stem cell niche including topographical and chemical instructions. Nanotopographical clues that mimic the extracellular matrix (ECM) in vivo have shown to regulate stem cell differentiation. The delivery of ECM components on biomaterials in the form of short peptides sequences has also proved successful in directing stem cell lineage. Growth factors responsible for controlling stem cell fate in vivo have also been delivered via biomaterials to provide clues to determine stem cell differentiation. An alternative approach to guide stem cells fate is to provide genetic clues including delivering DNA plasmids and small interfering RNAs via scaffolds. This review, aims to provide an overview of the topographical, chemical and molecular clues that biomaterials can provide to guide stem cell fate. The promising features and challenges of such approaches will be highlighted, to provide directions for future advancements in this exciting area of stem cell translation for regenerative medicine.

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Section: Neuronalmentioning

confidence: 99%

“…This study highlighted that MSC fate in 3D-culture can be controlled even in the absence of differentiation supplements [48] . Table 1 provides the summary of the key research studies highlighting the effect of nanotopographies on directing stem cell fate [49][50][51][52][53][54][55][56] .…”

mentioning

confidence: 99%

Control of stem cell fate by engineering their micro and nanoenvironment

Griffin

Butler

Seifalian

et al. 2015

WJSC

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“…They can be isolated from various tissues, such as bone marrow and adipose tissue, and comprise a subpopulation that is capable of differentiation towards multiple cell types of the mesodermal lineage including osteocytes, adipocytes, and chondrocytes [22]. It has been suggested that specific surface patterns can either maintain MSCs in an undifferentiated state [23] or drive them towards osteogenic [24], adipogenic [25], or chondrogenic lineages [26]. Cell shape, cytoskeletal tension and RhoA signaling are thought to direct this commitment [27].…”

Section: Introductionmentioning

confidence: 99%

Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages

et al. 2015

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“…Namely, the gene expression of osteocalcin, osteopontin, procollagen type I, alkaline phosphatase, and the transcription factors Runx2 and TGFβ-1 was consistently upregulated only in osteoblastic cells seeded on translationally ordered composite films made by dispersing HAp nanoparticles in a 80 kDa poly(ε-caprolactone) matrix, as opposed to translationally disordered and flat surfaces of the same composition 233 . Many prior studies have shown that topography can be a more important determinant of the viability of the biomaterial/tissue interface than the surface chemistry or stiffness 234,235,236 . This finding has, however, implied that the order at the level of the distribution of topographic features can be a more decisive factor than their surface density, size and geometry.…”

Section: Additional Common Ingredients Of Composite Biomaterialsmentioning

confidence: 99%

When 1 + 1 > 2: Nanostructured composites for hard tissue engineering applications

Uskoković

2015

Materials Science and Engineering: C

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Multicomponent, synergistic and multifunctional nanostructures have taken over the spotlight in the realm of biomedical nanotechnologies. The most prospective materials for bone regeneration today are almost exclusively composites comprising two or more components that compensate for the shortcomings of each one of them alone. This is quite natural in view of the fact that all hard tissues in the human body, except perhaps the tooth enamel, are composite nanostructures. This review article highlights some of the most prospective breakthroughs made in this research direction, with the hard tissues in main focus being those comprising bone, tooth cementum, dentin and enamel. The major obstacles to creating collagen/apatite composites modeled after the structure of bone are mentioned, including the immunogenicity of xenogeneic collagen and continuously failing attempts to replicate the biomineralization process in vitro. Composites comprising a polymeric component and calcium phosphate are discussed in light of their ability to emulate the soft/hard composite structure of bone. Hard tissue engineering composites created using hard material components other than calcium phosphates, including silica, metals and several types of nanotubes, are also discoursed on, alongside additional components deliverable using these materials, such as cells, growth factors, peptides, antibiotics, antiresorptive and anabolic agents, pharmacokinetic conjugates and various cell-specific targeting moieties. It is concluded that a variety of hard tissue structures in the body necessitates a similar variety of biomaterials for their regeneration. The ongoing development of nanocomposites for bone restoration will result in smart, theranostic materials, capable of acting therapeutically in direct feedback with the outcome of in situ disease monitoring at the cellular and subcellular scales. Progress in this research direction is expected to take us to the next generation of biomaterials, designed with the purpose of fulfilling Daedalus’ dream - not restoring the tissues, but rather augmenting them.

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Substrate topography determines the fate of chondrogenesis from human mesenchymal stem cells resulting in specific cartilage phenotype formation

Cited by 109 publications

References 44 publications

Control of stem cell fate by engineering their micro and nanoenvironment

Control of stem cell fate by engineering their micro and nanoenvironment

Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages

When 1 + 1 > 2: Nanostructured composites for hard tissue engineering applications

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